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Database: UniProt
Entry: Q9X759
LinkDB: Q9X759
Original site: Q9X759 
ID   ATSA_KLEPN              Reviewed;         577 AA.
AC   Q9X759;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   26-NOV-2014, entry version 61.
DE   RecName: Full=Arylsulfatase;
DE            Short=AS;
DE            EC=3.1.6.1;
DE   AltName: Full=Aryl-sulfate sulphohydrolase;
DE   Flags: Precursor;
GN   Name=atsA;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 10031 / DSM 681 / NBRC 3512 / NCIMB 9111 / NCTC 7427;
RX   PubMed=10336424; DOI=10.1074/jbc.274.22.15375;
RA   Szameit C., Miech C., Balleininger M., Schmidt B., von Figura K.,
RA   Dierks T.;
RT   "The iron sulfur protein AtsB is required for posttranslational
RT   formation of formylglycine in the Klebsiella sulfatase.";
RL   J. Biol. Chem. 274:15375-15381(1999).
RN   [2]
RP   PROTEIN SEQUENCE OF 63-76, OXOALANINE AT SER-72, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RC   STRAIN=ATCC 10031 / DSM 681 / NBRC 3512 / NCIMB 9111 / NCTC 7427;
RX   PubMed=9478923; DOI=10.1074/jbc.273.9.4835;
RA   Miech C., Dierks T., Selmer T., von Figura K., Schmidt B.;
RT   "Arylsulfatase from Klebsiella pneumoniae carries a formylglycine
RT   generated from a serine.";
RL   J. Biol. Chem. 273:4835-4837(1998).
CC   -!- FUNCTION: Plays an important role in the mineralization of
CC       sulfates.
CC   -!- CATALYTIC ACTIVITY: A phenol sulfate + H(2)O = a phenol + sulfate.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC       Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:10336424}.
CC   -!- PTM: The conversion to 3-oxoalanine (also known as C-
CC       formylglycine, FGly), of a serine or cysteine residue in
CC       prokaryotes and of a cysteine residue in eukaryotes, is critical
CC       for catalytic activity. {ECO:0000269|PubMed:9478923}.
CC   -!- SIMILARITY: Belongs to the sulfatase family. {ECO:0000305}.
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DR   EMBL; AJ131525; CAB40961.1; -; Genomic_DNA.
DR   PIR; T45548; T45548.
DR   ProteinModelPortal; Q9X759; -.
DR   BindingDB; Q9X759; -.
DR   ChEMBL; CHEMBL2189123; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-KW.
DR   GO; GO:0004065; F:arylsulfatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.720.10; -; 1.
DR   InterPro; IPR017849; Alkaline_Pase-like_a/b/a.
DR   InterPro; IPR017850; Alkaline_phosphatase_core.
DR   InterPro; IPR000917; Sulfatase.
DR   InterPro; IPR024607; Sulfatase_CS.
DR   Pfam; PF00884; Sulfatase; 1.
DR   SUPFAM; SSF53649; SSF53649; 1.
DR   PROSITE; PS00523; SULFATASE_1; 1.
DR   PROSITE; PS00149; SULFATASE_2; 1.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Hydrolase; Metal-binding;
KW   Periplasm; Signal.
FT   SIGNAL        1     20       {ECO:0000255}.
FT   CHAIN        21    577       Arylsulfatase.
FT                                /FTId=PRO_0000033448.
FT   ACT_SITE    134    134       {ECO:0000250}.
FT   METAL        34     34       Calcium. {ECO:0000250}.
FT   METAL        35     35       Calcium. {ECO:0000250}.
FT   METAL        72     72       Calcium; via 3-oxoalanine.
FT   METAL       329    329       Calcium. {ECO:0000250}.
FT   METAL       330    330       Calcium. {ECO:0000250}.
FT   MOD_RES      72     72       3-oxoalanine (Ser).
FT                                {ECO:0000269|PubMed:9478923}.
SQ   SEQUENCE   577 AA;  64156 MW;  7E897EDB2CABD18C CRC64;
     MNKKAMAAAV SMILAGGAHA AQQERPNVIV IIADDMGYSD ISPFGGEIPT PNLQAMAEQG
     MRMSQYYTSP MSAPARSMLL TGNSNQQAGM GGMWWYDSTI GKEGYELRLT DRVTTMAERF
     KDAGYNTLMA GKWHLGFVPG ATPKERGFNH AFAFMGGGTS HFNDAIPLGT VEAFHTYYTR
     DGERVSLPDD FYSSEAYARQ MNSWIKATPK EQPVFAWLAF TAPHDPLQAP DEWIKRFKGQ
     YEQGYAEVYR QRIARLKALG IIHDDTPLPH LELDKEWEAL TPEQQKYTAK VMQVYAAMIA
     NMDAQIGTLM ETLKQTGRDK NTLLVFLTDN GANPAQGFYY ESTPEFWKQF DNSYDNVGRK
     GSFVSYGPHW ANVSNAPYAN YHKTTSAQGG INTDFMISGP GITRHGKIDA STMAVYDVAP
     TLYEFAGIDP NKSLAKKPVL PMIGVSFKRY LTGEVQEPPR GNYGVELHHQ AAWVDGEWKL
     RRLVPRGLTA GDAPWQLFNL HDDPLETHDV AAEHPDRVKA MSEAYEAFAK RTMVTKAQGK
     MIDYVGIDSK TGRYLAVDPA TMKPVPAPQA IPVSEIH
//
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