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Database: UniProt
Entry: R0FI98_9BRAS
LinkDB: R0FI98_9BRAS
Original site: R0FI98_9BRAS 
ID   R0FI98_9BRAS            Unreviewed;       920 AA.
AC   R0FI98;
DT   26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT   26-JUN-2013, sequence version 1.
DT   07-JUN-2017, entry version 18.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN   ORFNames=CARUB_v10002637mg {ECO:0000313|EMBL:EOA22092.1};
OS   Capsella rubella.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Capsella.
OX   NCBI_TaxID=81985 {ECO:0000313|EMBL:EOA22092.1, ECO:0000313|Proteomes:UP000029121};
RN   [1] {ECO:0000313|Proteomes:UP000029121}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23749190; DOI=10.1038/ng.2669;
RA   Slotte T., Hazzouri K.M., Agren J.A., Koenig D., Maumus F., Guo Y.L.,
RA   Steige K., Platts A.E., Escobar J.S., Newman L.K., Wang W.,
RA   Mandakova T., Vello E., Smith L.M., Henz S.R., Steffen J., Takuno S.,
RA   Brandvain Y., Coop G., Andolfatto P., Hu T.T., Blanchette M.,
RA   Clark R.M., Quesneville H., Nordborg M., Gaut B.S., Lysak M.A.,
RA   Jenkins J., Grimwood J., Chapman J., Prochnik S., Shu S., Rokhsar D.,
RA   Schmutz J., Weigel D., Wright S.I.;
RT   "The Capsella rubella genome and the genomic consequences of rapid
RT   mating system evolution.";
RL   Nat. Genet. 45:831-835(2013).
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       conversion of glucosylceramide to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY: D-glucosyl-N-acylsphingosine + H(2)O = D-
CC       glucose + N-acylsphingosine. {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase
CC       family. {ECO:0000256|PIRNR:PIRNR028944}.
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DR   EMBL; KB870810; EOA22092.1; -; Genomic_DNA.
DR   RefSeq; XP_006289194.1; XM_006289132.1.
DR   GeneID; 17883701; -.
DR   KEGG; crb:CARUB_v10002637mg; -.
DR   KO; K17108; -.
DR   Proteomes; UP000029121; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   InterPro; IPR008928; 6-hairpin_glycosidase-like.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000029121};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000029121}.
FT   DOMAIN       85    402       Glyco_hydr_116N. {ECO:0000259|Pfam:
FT                                PF12215}.
FT   DOMAIN      492    852       DUF608. {ECO:0000259|Pfam:PF04685}.
SQ   SEQUENCE   920 AA;  104279 MW;  543CDBC7F52FC14F CRC64;
     MENSHMESEL QTQMVGNERL PQVTWQRKLN SKVKNPSEFK MSIKDYLHLF PIGYRLWRYT
     KEQAVKGRAS MYDMFKKRHI RGDHGVPLGG IGGGSIGRSY KGEFQQFKFF PNVCEEAPIL
     TNQFSVFVSR PGDVSYSTVL CPTKPESLNG KTEDLGIESW DWNMKGDKSM YHALYPRSWT
     VYNEPDPELR IVSRQVSPFI PQNYKESSLP VSVFDFTMSN LGKEETTVTL LFTWENSVGG
     ASGLTGEHFN STVMESNGVH AIALHHKTAN GHPPVTYSIA ARETEDVHVS ECPCFLVSGH
     SPKEITAKDM WDEIKKNKSF DGLNSEPGSR SRPGTSIGAA ISAKVKVPPG CDRTVTFSLS
     WDCPEVRFNE KTYHRRYTKF YGNLGDAAVK MARDALLSYV DWEFQIEAWQ SPILSDTTLP
     DWYRVTLFNE LYYFNSGGTI WTDGLPAKES IERSKILNTE QNKIVLDLFE KINAVCEQIH
     SPQTLKEEEN IGQFIYLEGV EYRMYNTYDV HFYSSFALLS LFPKLALSVQ RDFAATVLFQ
     DHTKKQIMSY GEWVPRKLLG SVPHDIGLND PWHELNEYNF FNTDRWKDLN AKFVLQVYRD
     VVATGDQSFA KAVWPSVYTA VAYLDQFDKD EDGMIENEGF PDQTYDAWSV TGVSAYCGGL
     WVAALQAASA LASIVGENTV AIYFNAKYEK AKRVYEKLWN GSYFNYDDSG SGSSSSILAD
     QLAGQWYARA CGLKPITKDE WIKKALETIY EFNVMKVRGG TRGAVNGMSP DGKVDTNSLV
     SKEVWAGTTY SVAACMIQEG QRDKGFKTAS GIYEAVWSDR GISCSFQTPE AWNMNDEYRS
     LCYMRPLAIW AIQWGLTRTG SVEADKLKLV EGKEEESDTL SRQHKGFKDV AHFVKILPTS
     NEHRSRLQDM YEAVLKTLRL
//
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