ID R0G027_9BRAS Unreviewed; 764 AA.
AC R0G027;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=RNA uridylyltransferase {ECO:0000256|ARBA:ARBA00012472};
DE EC=2.7.7.52 {ECO:0000256|ARBA:ARBA00012472};
GN ORFNames=CARUB_v10024989mg {ECO:0000313|EMBL:EOA28757.1};
OS Capsella rubella.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Capsella.
OX NCBI_TaxID=81985 {ECO:0000313|EMBL:EOA28757.1, ECO:0000313|Proteomes:UP000029121};
RN [1] {ECO:0000313|Proteomes:UP000029121}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Monte Gargano {ECO:0000313|Proteomes:UP000029121};
RX PubMed=23749190; DOI=10.1038/ng.2669;
RA Slotte T., Hazzouri K.M., Agren J.A., Koenig D., Maumus F., Guo Y.L.,
RA Steige K., Platts A.E., Escobar J.S., Newman L.K., Wang W., Mandakova T.,
RA Vello E., Smith L.M., Henz S.R., Steffen J., Takuno S., Brandvain Y.,
RA Coop G., Andolfatto P., Hu T.T., Blanchette M., Clark R.M., Quesneville H.,
RA Nordborg M., Gaut B.S., Lysak M.A., Jenkins J., Grimwood J., Chapman J.,
RA Prochnik S., Shu S., Rokhsar D., Schmutz J., Weigel D., Wright S.I.;
RT "The Capsella rubella genome and the genomic consequences of rapid mating
RT system evolution.";
RL Nat. Genet. 45:831-835(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=RNA(n) + UTP = diphosphate + RNA(n)-3'-uridine ribonucleotide;
CC Xref=Rhea:RHEA:14785, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17348,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:46398, ChEBI:CHEBI:140395,
CC ChEBI:CHEBI:173116; EC=2.7.7.52;
CC Evidence={ECO:0000256|ARBA:ARBA00024498};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; KB870808; EOA28757.1; -; Genomic_DNA.
DR RefSeq; XP_006295859.1; XM_006295797.1.
DR AlphaFoldDB; R0G027; -.
DR STRING; 81985.R0G027; -.
DR GeneID; 17888111; -.
DR KEGG; crb:17888111; -.
DR eggNOG; KOG2277; Eukaryota.
DR OrthoDB; 170176at2759; -.
DR Proteomes; UP000029121; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:InterPro.
DR GO; GO:0006402; P:mRNA catabolic process; IEA:UniProt.
DR GO; GO:0061157; P:mRNA destabilization; IEA:UniProt.
DR CDD; cd05402; NT_PAP_TUTase; 1.
DR Gene3D; 1.10.1410.10; -; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR002058; PAP_assoc.
DR InterPro; IPR045100; TUTase_dom.
DR PANTHER; PTHR12271; POLY A POLYMERASE CID PAP -RELATED; 1.
DR PANTHER; PTHR12271:SF40; POLY(A) RNA POLYMERASE GLD2; 1.
DR Pfam; PF03828; PAP_assoc; 1.
DR Pfam; PF19088; TUTase; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF81631; PAP/OAS1 substrate-binding domain; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000029121};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 448..548
FT /note="TUTase nucleotidyltransferase"
FT /evidence="ECO:0000259|Pfam:PF19088"
FT DOMAIN 656..714
FT /note="PAP-associated"
FT /evidence="ECO:0000259|Pfam:PF03828"
FT REGION 1..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 197..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 333..369
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 381..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..38
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 201..216
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 336..364
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 764 AA; 85898 MW; 2238415C97301620 CRC64;
MADGGAASPA PPSENVGDFI LSLLQQNTRP SPSQQGGPPQ HLDPAIAAVG PTVNPFPPSI
WQSSNGRDHR PGTLNPSWPH AAFSPPPNLS PNLLGFPQFT PNPFPLNQFD GNQRLSPEDA
YRLGFPATGT HAIQSMVQQQ QPPPPPQSDY RKLVFGSFSG DATQSLNGLR NGNLKYDSIH
QEQLMRNPQS VVLNSNPEDP NLSHHRNHDL HEQRGGHNGR GGNWGPIGNN VRGFKSTPTP
PPPGFSSNQR GWDMNLGSKD DDRGIGSFQR NHDRAMWEHS NLNAEADRLR GLSLQNESKF
NLSQQIDHPG PPKGTSLHSV STADAANSFS MLNKEARGGS ERKDELGQLS KMKREGNEKS
GPGDDEIDDF GEDIVDSLLL EVDTDDKDAK DGKKNSKTSR EKESRVDNRG RWLLSQRLRE
RKMYMACRND IHRYDAPFMA VYKSLIPAEE ELEKQRQLMA QLENLVAKEW PHAKLYLYGS
CANSFGFPKS DIDVCLAIED DDINKSDMLL KLADILESDN LQNVQALTRA RVPIVKLMDP
VTGISCDICI NNVLAVVNTK LLRDYARIDV RLRQLAFIVK HWAKSRKVNE TYQGTLSSYA
YVLMCIHFLQ LRRPPILPCL QEMKPTYSVR VDNIRCSYFD DVGRLDNFGS SNRETIAELV
WGFFNYWAYA HDYANTVVSV RTGSILGKRE KDWTRRVGND RHLICIEDPF ETSHDLGRVV
DKFSIRVLRE EFERAAEIMH QDRNPCAKLF EPYLPEDNNG QGHK
//
