ID R1AB_CVTMI Reviewed; 306 AA.
AC Q9WQ76;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 08-NOV-2023, entry version 76.
DE RecName: Full=Replicase polyprotein 1ab;
DE Short=pp1ab;
DE AltName: Full=ORF1ab polyprotein;
DE Contains:
DE RecName: Full=RNA-directed RNA polymerase;
DE Short=Pol;
DE Short=RdRp;
DE EC=2.7.7.48;
DE AltName: Full=nsp12;
DE Flags: Fragment;
GN Name=rep; ORFNames=1a-1b;
OS Turkey coronavirus (strain Minnesota) (TCoV) (TCV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Gammacoronavirus; Igacovirus; Avian coronavirus.
OX NCBI_TaxID=231430;
OH NCBI_TaxID=9103; Meleagris gallopavo (Wild turkey).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=10392726; DOI=10.1016/s0168-1702(99)00017-9;
RA Stephensen C.B., Casebolt D.B., Gangopadhyay N.N.;
RT "Phylogenetic analysis of a highly conserved region of the polymerase gene
RT from 11 coronaviruses and development of a consensus polymerase chain
RT reaction assay.";
RL Virus Res. 60:181-189(1999).
CC -!- FUNCTION: The replicase polyprotein of coronaviruses is a
CC multifunctional protein: it contains the activities necessary for the
CC transcription of negative stranded RNA, leader RNA, subgenomic mRNAs
CC and progeny virion RNA as well as proteinases responsible for the
CC cleavage of the polyprotein into functional products.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Ribosomal frameshifting; Named isoforms=2;
CC Name=Replicase polyprotein 1ab; Synonyms=pp1ab;
CC IsoId=Q9WQ76-1; Sequence=Displayed;
CC Name=Replicase polyprotein 1a; Synonyms=pp1a, ORF1a polyprotein;
CC IsoId=Q9WQ76-2; Sequence=Not described;
CC -!- MISCELLANEOUS: [Isoform Replicase polyprotein 1ab]: Produced by -1
CC ribosomal frameshifting at the 1a-1b genes boundary.
CC -!- MISCELLANEOUS: [Isoform Replicase polyprotein 1a]: Produced by
CC conventional translation. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the coronaviruses polyprotein 1ab family.
CC {ECO:0000305}.
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DR EMBL; AF124991; AAD32995.1; -; Genomic_RNA.
DR SMR; Q9WQ76; -.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR046441; RdRp_CoV.
DR InterPro; IPR001205; RNA-dir_pol_C.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF00680; RdRP_1; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR PROSITE; PS51948; COV_NSP12_RDRP; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 3: Inferred from homology;
KW Nucleotide-binding; Nucleotidyltransferase; Ribosomal frameshifting;
KW RNA-binding; RNA-directed RNA polymerase; Transferase;
KW Viral RNA replication.
FT CHAIN <1..>306
FT /note="RNA-directed RNA polymerase"
FT /id="PRO_0000037403"
FT DOMAIN <1..>306
FT /note="Nsp12 RNA-dependent RNA polymerase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01293"
FT DOMAIN 106..268
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT REGION 1..75
FT /note="RdRp Fingers N-ter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01293"
FT REGION 76..114
FT /note="RdRp Palm N-ter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01293"
FT REGION 115..173
FT /note="RdRp Fingers C-ter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01293"
FT REGION 174..>306
FT /note="RdRp Palm C-ter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01293"
FT REGION ?306..>306
FT /note="RdRp Thumb"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01293"
FT NON_TER 1
FT NON_TER 306
SQ SEQUENCE 306 AA; 34874 MW; 52E573B1D84F13FE CRC64;
FNKFGKARLY YEMSLEEQDQ LFESTKKNVL PTITQMNLKY AISAKNRART VAGVSILSTM
TNRQFHQKIL KSIVNTRNAP VVIGTTKFYG GWDNMLRNLI QGVEDPILMG WDYPKCDRAM
PNLLRITASL VLARKHTNCC TWSERIYRLY NECAQVLSET VLATGGIYVK PGGTSSGDAT
TAYANSVFNI IQATSANVAR LLSVITRDIV YDDIKSLQYE LYQQVYRRVN FDPAFVEKFY
SYLCKNFSLM IFADDGVVCY NNTLAKQGLV ADISGFREIL YYQNNVYMAD SKCWVEPDLE
KGPHEF
//
