ID R1E6P3_BOTPV Unreviewed; 3786 AA.
AC R1E6P3;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 68.
DE SubName: Full=Putative pks-nrps protein {ECO:0000313|EMBL:EOD43393.1};
GN ORFNames=UCRNP2_9904 {ECO:0000313|EMBL:EOD43393.1};
OS Botryosphaeria parva (strain UCR-NP2) (Grapevine canker fungus)
OS (Neofusicoccum parvum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetes incertae sedis; Botryosphaeriales; Botryosphaeriaceae;
OC Neofusicoccum.
OX NCBI_TaxID=1287680 {ECO:0000313|EMBL:EOD43393.1, ECO:0000313|Proteomes:UP000013521};
RN [1] {ECO:0000313|Proteomes:UP000013521}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCR-NP2 {ECO:0000313|Proteomes:UP000013521};
RX PubMed=23766404; DOI=10.1128/genomea.00339-13;
RA Blanco-Ulate B., Rolshausen P., Cantu D.;
RT "Draft genome sequence of Neofusicoccum parvum isolate UCR-NP2, a fungal
RT vascular pathogen associated with grapevine cankers.";
RL Genome Announc. 1:E0033913-E0033913(2013).
CC -!- SIMILARITY: In the C-terminal section; belongs to the NRP synthetase
CC family. {ECO:0000256|ARBA:ARBA00029443}.
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DR EMBL; KB916836; EOD43393.1; -; Genomic_DNA.
DR RefSeq; XP_007589136.1; XM_007589074.1.
DR STRING; 1287680.R1E6P3; -.
DR KEGG; npa:UCRNP2_9904; -.
DR eggNOG; KOG1178; Eukaryota.
DR eggNOG; KOG1202; Eukaryota.
DR HOGENOM; CLU_000022_37_4_1; -.
DR OMA; ETDVHHA; -.
DR OrthoDB; 5396558at2759; -.
DR Proteomes; UP000013521; Unassembled WGS sequence.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:0018130; P:heterocycle biosynthetic process; IEA:UniProt.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR GO; GO:0009403; P:toxin biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd19532; C_PKS-NRPS; 1.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 1.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR013120; Far_NAD-bd.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 1.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF20; HYBRID PKS-NRPS SYNTHETASE APDA; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF00668; Condensation; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF07993; NAD_binding_4; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 2.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SMART; SM00823; PKS_PP; 2.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 2.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS52004; KS3_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000013521};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 5..438
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2380..2457
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3437..3513
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 2352..2380
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2460..2486
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3786 AA; 411322 MW; B6647F26BB67EA25 CRC64;
MAPTKEPIAI IGSGCRFPGG SSSPSKLWDL LRNPRDVLRE FPEDRLRLPN FYNKNGEHHG
STDVKNKGYL LSEDHRVFDA PFFNINPLEA DGMDPAQRIL LETVYEALEA AGYSIEEMQG
TLTSVFVGLM TADWWDLQMR DTETIATYAA TGTARSIISN RISYFFDLKG ASMTIDTACS
SSLVALHQAV QSLRNGESTT AVVAGANVLL DPSMYIAESK LHMLSPESRS RMWDKSADGY
ARGEGCAAVF LKPLSKAIAD GDHIECVVRE TGVNSDGRTK GITMPSATAQ AALIRATYQS
AGLDPLVDRC QYFECHGTGT AAGDPIEAQA ISEAFFSGAQ PEESEEKMYV GSIKTVIGHL
EGCAGLAGLL KASLAIQNRT VPANLLFNEL NPAIEPYYRH LQILQSGIPW PTVSDGPLRA
SVNSFGFGGT NAHAILESYE PAAAAQPQTA ETETEHFRGP LTFSARSESS LLATIRAFTD
HVKANPAADL ENLAWVLQRR RGAFPVKHFF SGGTRERLVS FMESFLATAD SGSVGGLVTR
AQLRYPDEAP GILGVFTGQG AQWATMGTAL IARSRVFRES IARSEAALAA LPDPPAWSLR
AELLAGKETS RLAEAELSQP LCTALQIATV DLVGAAGVRL DAVVGHSSGE IAAAYAAGML
SAADAMAIAY YRGFHAKLAQ GTDGKKGGMM AVGLSYEAAL GFCAQPRWAG RIGLAASNSP
SSVTLSGDLD AVLEAKEFFD GEKTFARQLL VDTAYHSYHM LPCAAPYLES LRRCNITVHR
PRPGCVWSSS VRGDTELLEG DLAALAGPYW VDNMVQPVLF SQAVECSLWN GGPFEMVVEI
GPHPALKGPA TQVLKAVLGS APPYMGFMRR GDDEVEAFSG GLGFLWSNLG PAFVDFDGYR
AACADVDAAA VRPTMLKGLP SYAWDHDKVY WKEGRISRNF RLRRSTPHEL LGRRVPDDSD
YEMRWRNVLR LNEIPWIRGH EFQGQVLFPA AAYIAMALEV SKTLAGDRPV RLFELQDISI
RKALVMDEDS PGVETVFTVK LLNKEFRAND TGKDVLEAEF SGYTCADESA GVLEKTACGR
IVVHLGERTG DELPPVAPAR PHLAPVDLER FYSSIANLGL NYQGLFQGLT HAERTLGYSR
AAATWQNHDM AAEYMVHPAF LDVVFQAIFV ALTSPATNGA LWTPYLPIAL HRIIVDPTQV
HDYSLPQVRA EMDAFITQSS SSSVVGDVHL LDPTGTHTSI QVEGLFLKSI SEATPANDRL
LFSQTVWDAD IANGAADAVE GEEDEDEREL IHAIERLALF HFQDLAKTIP AEEVKTFTWY
HQRLFEAIEA NLESVRNGQN PVIEQAWLND TRETIDNLRE KFPGQIDLEL MHAISENLVS
VVRGETQILE VMLQNDMLNR FYMEGRGFRK LNDYIARVVQ QIAHKHPRAK FLEIGAGTGG
TTRNIFDTIG STYSSYTYTD ISSGFFEKAA EKFHDHVNKM NFKVLNIEKD VAGQGFQEGS
YDVIIAANVL HATRTLEETM QHTRSLLKPG GYFILMEVTG DLLRNPFVMG ALEGWWLGAE
EGRRLSPGIS TVEWDDLLQS TGFSGVNTVL HDFPDVTRHS CSVMVSQAVD DRFSLLREPL
SAMTMFLEEQ VLIIGGKTLP VSKLVRDVQK VWRQRVTVVN SIDALNPAKI APRTSVICLT
ELDKPLFAEP MTEARLNKLQ ELFITAKSVL WVTSGCSEDS PVSNMAVGIG RALITELPHL
TLQFLDVKRI SSLTPTFIVE SFLRLIIAGS PEVAEHNMLW TTEPEIALDD DAVLIPRVVP
DKTINDRFNA ARRLVTKSVS KAETRVEIAA SGAEESLSLL EAVEPVDPTK TVVDVKYSTA
LGKSHTLALG EVRGTSQLAL AVSVADSSTI NASDADIFVI PDGHKASAAT LLDVASNVLA
LELLKLVPAN RSALIHEPTD RLGAAIKHVA QVKGIEVFLI TSKESKKDGW LSIHPHATER
ALRNIVPRTV GAFIDLSSSP FTALASSLPQ GASMHTFDSF NGIVSSLDSH LLSAAFKETL
SSSLGETVAA VVEAQNLAGA PRTSNIYPNV VDWTEDKLTV AVKPLDTTGV FRSDKTYLMV
GMTGEMGRSL CRWMVTNGAR YIVLTSRSAE VNEAWLGEMA DLGATVKVYK MDVSDRSSVR
SVHATIKETL PPIAGVCNAA MVLIDKLFVD MNAETLNNVL GPKVEGTKIL DELFNEPNLD
FFVLFSSLAS IFGNAGQSNY HAANLFMTSL AAQRRARGLA ASVMHIGMVA DVGYVARTGR
HIEDHLRKLL FHPMSETDVQ HLFAEAVMSS RPETQRNWSI VSGIEPFLDT PDATSRPPFY
SNPRFAHYIH EENASKEQKQ SSSAGRDVKQ QLEEASSEED ATAAIQEAFA AKLEAMLQMA
PNTVNVNVPL LDLGTDSLLA VEIRTWFLKT IHVDIPVLKV LSGDTVAAIC TDAARKYLAA
RPAKEQKPAA EEPSADAGSG KENVEAANTQ GDLKIMRFRR ALAEVIARHE SLRTAYFTDA
ETGEPTQGIT AVQPDCLKHV QDADEQVVTR EFNLLKNTNW SLEQGITFGA TVVSETSGRH
TIIFGYHHIV MDGVSWFLFL RDLSQAYALK PLQPVTKQYA DFTKEQAQSI ASGALTDKMS
FWKNEFSQLP EPIPLLPIAR VKSRWGAGSY DSHTASKELD AKFNAQIKES SRALGVSPFH
FHLATIQVLF AKFLNIEDVC IGVSDANRTS DDLSDTVGFF LNLLPLRFTV DRNGSFSDLA
KHTAKKVFSA LANSEVPFDL ILDQLNVARS RAHSPLFQVG VNYRMNDLLT IPFGDCQLQI
AAVEDAKNPY DISFSITQSA NGTCMVDITC REDLYTAEAP RMLLDVYNHL LKSLSDDHFT
SVETCSLFDD AEGHKSVLIG RGPRVASEWQ ATLTERFEFV AEHSPDAVAI KDSHGSLTYH
QLADKADDIA ATLLKAGATS GSPVAVLCQP SSNIVASLLA ILRIGGIYVP LDLSLPQARH
AAMLANCKPS LLICDADTSA RAHETAPEAS IVNISDLAEI DTQDVKTLPK PSSVSPAFLL
YTSGSTGTPK GIYLPQRGIL NWCEQCSKIL SVTPQDSVML QTSFGFDMSI VEIFLALGNG
SRLVIVPQAA RGDPVAISEL MLEEKVTFTC ATPSEYLMLL ANGEAALRQC SAWVKAVSGG
ESVTERMKEA FRKLDRPDFV LTNWYGPTEI SAASTFEHVD LTAPAAGADS GYASIGKPVP
NYSIYILDEA GKPVPVGMPG EICVGGAGVA LGYLGLPEQT EAKFRPDTFA DADDVARGWT
TMYRTADKGR LQSDGSVIFM GRMDGDTQIK LRGLRIELGD IENALLQAAP GTLSGAVVSV
RGDPELLVAH IVFAPGKTLD GPALQLLASD LPLPQYMRPA MVIPLDRLPI TNNGKVDRKA
IEALALPDQP NETQSQATLT LPESRLVRLW KAVLPQGVSV EPSSDFFTCG GNSLLLVKLK
TAIKEEYGIS LPITRLYQAS TLGAMASLIA VQQEDQTRDE DINWDEEIAI PESIVLPTKP
ASSQHAVKDH DMEVLLTGAQ SFLGSTILTS LLTDPRVAKV HLVAVHPDGI ATLPSSDKIV
VYTGSLQHPT LNLSTSDIST LQSTTDLIIH AGASGHCLHN YSSIRAANLG ATHLLATLAL
PRRIPLHFLS SNRVTLLSGR TALPPISVST HRPPTDGSEG LTATKWACEA FLESVSSAAQ
LPVTVHRACA VVGDRAPPDD ALNSLLRYSL LLRAVPAFGG FDGFFDFRDV REVADEVRGE
GAGGGF
//
