ID   R1FVR6_BOTPV            Unreviewed;       730 AA.
AC   R1FVR6;
DT   26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT   26-JUN-2013, sequence version 1.
DT   27-MAR-2024, entry version 41.
DE   RecName: Full=Trehalase {ECO:0000256|RuleBase:RU361180};
DE            EC=3.2.1.28 {ECO:0000256|RuleBase:RU361180};
DE   AltName: Full=Alpha-trehalose glucohydrolase {ECO:0000256|RuleBase:RU361180};
GN   ORFNames=UCRNP2_10101 {ECO:0000313|EMBL:EOD43195.1};
OS   Botryosphaeria parva (strain UCR-NP2) (Grapevine canker fungus)
OS   (Neofusicoccum parvum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetes incertae sedis; Botryosphaeriales; Botryosphaeriaceae;
OC   Neofusicoccum.
OX   NCBI_TaxID=1287680 {ECO:0000313|EMBL:EOD43195.1, ECO:0000313|Proteomes:UP000013521};
RN   [1] {ECO:0000313|Proteomes:UP000013521}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCR-NP2 {ECO:0000313|Proteomes:UP000013521};
RX   PubMed=23766404; DOI=10.1128/genomea.00339-13;
RA   Blanco-Ulate B., Rolshausen P., Cantu D.;
RT   "Draft genome sequence of Neofusicoccum parvum isolate UCR-NP2, a fungal
RT   vascular pathogen associated with grapevine cankers.";
RL   Genome Announc. 1:E0033913-E0033913(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-
CC         glucose; Xref=Rhea:RHEA:32675, ChEBI:CHEBI:15377, ChEBI:CHEBI:15903,
CC         ChEBI:CHEBI:16551, ChEBI:CHEBI:17925; EC=3.2.1.28;
CC         Evidence={ECO:0000256|ARBA:ARBA00001576,
CC         ECO:0000256|RuleBase:RU361180};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 37 family.
CC       {ECO:0000256|ARBA:ARBA00005615, ECO:0000256|RuleBase:RU361180}.
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DR   EMBL; KB916884; EOD43195.1; -; Genomic_DNA.
DR   RefSeq; XP_007589332.1; XM_007589270.1.
DR   AlphaFoldDB; R1FVR6; -.
DR   STRING; 1287680.R1FVR6; -.
DR   KEGG; npa:UCRNP2_10101; -.
DR   eggNOG; KOG0602; Eukaryota.
DR   HOGENOM; CLU_006451_1_1_1; -.
DR   OMA; WLFMMTK; -.
DR   OrthoDB; 1329212at2759; -.
DR   Proteomes; UP000013521; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004555; F:alpha,alpha-trehalase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005993; P:trehalose catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001661; Glyco_hydro_37.
DR   InterPro; IPR018232; Glyco_hydro_37_CS.
DR   InterPro; IPR011120; Trehalase_Ca-bd.
DR   PANTHER; PTHR23403:SF6; CYTOSOLIC NEUTRAL TREHALASE-RELATED; 1.
DR   PANTHER; PTHR23403; TREHALASE; 1.
DR   Pfam; PF01204; Trehalase; 1.
DR   Pfam; PF07492; Trehalase_Ca-bi; 1.
DR   PRINTS; PR00744; GLHYDRLASE37.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS00927; TREHALASE_1; 1.
DR   PROSITE; PS00928; TREHALASE_2; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361180};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361180};
KW   Reference proteome {ECO:0000313|Proteomes:UP000013521}.
FT   DOMAIN          85..114
FT                   /note="Neutral trehalase Ca2+ binding"
FT                   /evidence="ECO:0000259|Pfam:PF07492"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          56..75
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   730 AA;  83692 MW;  C41ED6DDF22AB14A CRC64;
     MSSQQSVPRH HRSSTNDIDP LSAAQIYYGD SHKKKTKTRT YSGVLDVGQH NAAVAAGMKQ
     PGRRSSHDEH ASQPRKFLIP VDETLKTLLE REDTDQNYQI TIEDSGPKVL SVGTAASNGL
     NRFDVRGTYM LANLLQELTL AKDYGRKTIV LDEARLNENP VNRLSRLIKD TFWNNLTRRT
     DSSNIARIAR DPKDWTENPH PRIYVPRGAP EQYEYYLKHA KEHPDDKLEV IWLAEDCTND
     DYVRDLNDAP GLLALDMEEY INEQGEKDFR GKPFVVPGGR FNELYGWDSY MESLGLLVND
     RVDLVKSMVE NFCFCIKHYG KILNGNRTYY LGRSQPPFLT DMCLRVYDRI KHEPGAQDFL
     KTSIMAAIKE YYSVWMAAPR YDPESGLTRY RPGGIGVPPE TEATHFVHIL EPYAKKYNMT
     FEEFVQAYNH RKVSEPELDM YFLHDRAVRE SGHDTSYRLE RVAADLATVD LNSLLYKYET
     DIARTIRAHF GDRLAIPAEF CVPGQEPGHV ETSAVWDRRA KKRKALMEKY MWSEEEGMFF
     DYHTVEKKQT GYESATTFWP MWAGVATPRQ AAALVTKALP KLEEFGGLVS GTEKSRGEVG
     LERPNRQWDY PFGWAPQQML AWVGLQRFGY EAEAQRLAYK WCYMVTKAFV DFNGVVVEKY
     DVTRPIDPHK VEAEYGNQGS DFKGVPREGF GWVNASYVYG LQMLNAHMRR ALGALTPYDT
     FVRATEALGF
//