ID R3W269_9ENTE Unreviewed; 588 AA.
AC R3W269;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:EOL41767.1};
GN ORFNames=UC3_03332 {ECO:0000313|EMBL:EOL41767.1};
OS Enterococcus phoeniculicola ATCC BAA-412.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=1158610 {ECO:0000313|EMBL:EOL41767.1, ECO:0000313|Proteomes:UP000013785};
RN [1] {ECO:0000313|EMBL:EOL41767.1, ECO:0000313|Proteomes:UP000013785}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-412 {ECO:0000313|EMBL:EOL41767.1,
RC ECO:0000313|Proteomes:UP000013785};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A.M., Gilmore M.S., Lebreton F., Walker B., Young S.K., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A.,
RA Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Enterococcus phoeniculicola BAA-412.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOL41767.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJAT01000018; EOL41767.1; -; Genomic_DNA.
DR RefSeq; WP_010769963.1; NZ_KB946329.1.
DR AlphaFoldDB; R3W269; -.
DR STRING; 154621.RV11_GL002706; -.
DR PATRIC; fig|1158610.3.peg.3324; -.
DR eggNOG; COG1874; Bacteria.
DR HOGENOM; CLU_007853_7_2_9; -.
DR OrthoDB; 9813184at2; -.
DR Proteomes; UP000013785; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000013785}.
FT DOMAIN 8..327
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 374..486
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 506..564
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 156
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 237
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ SEQUENCE 588 AA; 67843 MW; 468AF0B47CE752D1 CRC64;
MNFEIKEEFY IDNQAVKIIS GAVHYFRIPP TQWKQTLYNL KAMGCNCVET YVPWNLHEPQ
KGVFDFSGLA DITKFLDSAQ ELGLYVIVRP SPYICAEWDF GGLPAWLLAT DGMRIRSQHP
DYLGAVSDYY EVLLPKLIPY QFTQGGNLLM VQVENEYGSY GEDKTYLKFL VKELRKYLDI
PLFTSDGGWK EVLDAGTLPE EGIFATANFG SDAKENFKWL KAYQDKHNIR APYMCMEFWD
GWFNSWQKPI IKREPNETAE EVRSVLKKGS INFYMFQGGT NYGFYNGCSD AGEKNEPQIT
SYDYDALLTE WGAPTEKYFA IQKVIKEEVP AAEISVPTYP KMTALGTFPV QESVGLFSVL
EEISEPFMND YTLPMEKVNQ NYGYTLYRSQ LQEKRFIPKL KVVDALDRIQ FFVNQEFIAT
QYQEDVGKEI SVELTKEENE LSLLVENMGR NNYGPKLVAD TQRKGIRGGV MEDIHYLSDW
AHYRLEFLPE QLAKIDFSKP SNEATPSFFK VEIELEETIN TFLDCSKFGK GVVFVNGQNM
GRYWKEGPVK TLYIADYFWK KGRNELIIFE TEGIAIQQID LIEEPIME
//
