ID R4K600_CLOPA Unreviewed; 526 AA.
AC R4K600;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=1,4-alpha-glucan branching enzyme {ECO:0008006|Google:ProtNLM};
GN ORFNames=Clopa_3174 {ECO:0000313|EMBL:AGK97988.1};
OS Clostridium pasteurianum BC1.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=86416 {ECO:0000313|EMBL:AGK97988.1, ECO:0000313|Proteomes:UP000013523};
RN [1] {ECO:0000313|EMBL:AGK97988.1, ECO:0000313|Proteomes:UP000013523}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BC1 {ECO:0000313|EMBL:AGK97988.1,
RC ECO:0000313|Proteomes:UP000013523};
RG US DOE Joint Genome Institute;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Mikhailova N., Teshima H., Detter J.C., Han C., Tapia R.,
RA Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Dunn J.,
RA Taghavi S., Francis A., van der Lelie D., Woyke T.;
RT "Complete sequence of chromosome of Clostridium pasteurianum BC1.";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 57 family.
CC {ECO:0000256|ARBA:ARBA00006821, ECO:0000256|RuleBase:RU361196}.
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DR EMBL; CP003261; AGK97988.1; -; Genomic_DNA.
DR RefSeq; WP_015616276.1; NC_021182.1.
DR AlphaFoldDB; R4K600; -.
DR STRING; 86416.Clopa_3174; -.
DR KEGG; cpas:Clopa_3174; -.
DR PATRIC; fig|86416.3.peg.3162; -.
DR eggNOG; COG1543; Bacteria.
DR HOGENOM; CLU_008192_1_0_9; -.
DR OrthoDB; 9803279at2; -.
DR Proteomes; UP000013523; Chromosome.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:InterPro.
DR GO; GO:0030979; P:alpha-glucan biosynthetic process; IEA:InterPro.
DR CDD; cd10792; GH57N_AmyC_like; 1.
DR Gene3D; 1.20.1430.10; Families 57/38 glycoside transferase, middle domain; 1.
DR Gene3D; 3.20.110.10; Glycoside hydrolase 38, N terminal domain; 1.
DR InterPro; IPR037090; 57_glycoside_trans_central.
DR InterPro; IPR015293; BE_C.
DR InterPro; IPR040042; Branching_enz_MT3115-like.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR004300; Glyco_hydro_57_N.
DR PANTHER; PTHR41695; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR PANTHER; PTHR41695:SF1; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR Pfam; PF09210; BE_C; 1.
DR Pfam; PF03065; Glyco_hydro_57; 1.
DR SUPFAM; SSF88688; Families 57/38 glycoside transferase middle domain; 1.
DR SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361196};
KW Reference proteome {ECO:0000313|Proteomes:UP000013523}.
FT DOMAIN 8..328
FT /note="Glycoside hydrolase family 57 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03065"
FT DOMAIN 425..524
FT /note="1,4-alpha-glucan branching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF09210"
FT ACT_SITE 189
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT ACT_SITE 350
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT BINDING 241
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 258
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 404
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 464
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
SQ SEQUENCE 526 AA; 61602 MW; 5943276DD7987337 CRC64;
MQKGYVSIVL HSHMPYIRHP EIKDPLEERW LFEAINECYI PLIQVYEGLI KDKISFKITM
SITPPLMSML QDEYLNERYT EYLKKSIELT EKELIRTKDN EELNKLAKFY NDRFNKLMKT
YEDYDCNLMN AFKKFDKLGC LEVITCSATH ALLPLIAINP EAVRAQLATG IQSYIDTMGH
SPNGIWLPEC AYTYSLDSVL KEFNIKYFIA ESKALLYADP KPLYGTSAPI STPNGVCAFG
RDMDSSYQVW SDFIGYPGDE NYREFYRDIG FELPMDYVKP YINAAGIRLD TGIKYHKITG
KTEKKQYYNR ERAMEKVREH AAHFAKCRQD QISGLSEHME VPPIITCPYD TELFGHWWFE
GPDFIDAFIR ESSKENYCYD FITPSEYLMN NSRVQCCSPN PSSWGENSDY SVWINSSNQW
IYRDLHRCEE IMIRLANTYK SPSELQTRAL NQAARELMLA ESSDWPFIIK NNTTVEYVIK
RINTHIDRFT RLYDSISKNI IDIKFLEKIE ALDNIFPDIN YKIYET
//
