ID   R5B4K4_9CLOT            Unreviewed;       909 AA.
AC   R5B4K4;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 27.
DE   SubName: Full=Penicillin-binding protein 2 {ECO:0000313|EMBL:CCX50223.1};
GN   ORFNames=BN545_01632 {ECO:0000313|EMBL:CCX50223.1};
OS   Clostridium sp. CAG:226.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1262781 {ECO:0000313|EMBL:CCX50223.1, ECO:0000313|Proteomes:UP000018047};
RN   [1] {ECO:0000313|EMBL:CCX50223.1, ECO:0000313|Proteomes:UP000018047}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:226 {ECO:0000313|Proteomes:UP000018047};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004167}; Single-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004167}.
CC   -!- SIMILARITY: Belongs to the transpeptidase family.
CC       {ECO:0000256|ARBA:ARBA00007171}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CCX50223.1}.
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DR   EMBL; CAWN010000119; CCX50223.1; -; Genomic_DNA.
DR   AlphaFoldDB; R5B4K4; -.
DR   Proteomes; UP000018047; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR   Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 2.
DR   Gene3D; 3.90.1310.10; Penicillin-binding protein 2a (Domain 2); 2.
DR   Gene3D; 1.10.10.1230; Penicillin-binding protein, N-terminal non-catalytic domain, head sub-domain; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR005311; PBP_dimer.
DR   InterPro; IPR036138; PBP_dimer_sf.
DR   InterPro; IPR001460; PCN-bd_Tpept.
DR   PANTHER; PTHR30627; PEPTIDOGLYCAN D,D-TRANSPEPTIDASE; 1.
DR   PANTHER; PTHR30627:SF2; PEPTIDOGLYCAN D,D-TRANSPEPTIDASE MRDA; 1.
DR   Pfam; PF03717; PBP_dimer; 1.
DR   Pfam; PF00905; Transpeptidase; 2.
DR   SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR   SUPFAM; SSF56519; Penicillin binding protein dimerisation domain; 1.
PE   3: Inferred from homology;
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018047}.
FT   DOMAIN          51..359
FT                   /note="Penicillin-binding protein dimerisation"
FT                   /evidence="ECO:0000259|Pfam:PF03717"
FT   DOMAIN          432..608
FT                   /note="Penicillin-binding protein transpeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF00905"
FT   DOMAIN          728..883
FT                   /note="Penicillin-binding protein transpeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF00905"
SQ   SEQUENCE   909 AA;  101403 MW;  0D0CE9FFD5E1A544 CRC64;
     MKKPISRIII VCLLMLCMMG ALIYRLGTLT IKEGEKWAQT ADSRSTQTIA IKGERGRIMD
     RNGVVLAYSE TCYNVEFLRN ADNRTSYDSA VYTESLIKAI EIIERSGGQT IDTSYIVMDE
     NGEIAYDWRI SGVESSEAIE RVRRIRYKNF CEGMYISIKD PAYKEYPKDP TKWDMDLWPT
     AEYAYNYLRK TWYIPEEYTF EQAKKIISIR QEVNLNNYRA YEPITIAYNV GQEVVSEIVE
     RSSELVGVQI AQSTTRVYPR GETAAHIVGY LSRNADTVSA AKLIAMGYTR AQLEPYYLKN
     DDGTYSMGDD GDYQIKMTDM GYAYSDYIGV SGLEYTMEAY LTGATNEHQG KREVEINKNL
     SITRELSYVE ATNGNDVMST IDIELQTVCE NALGSLITKM REQEQQMIEE DAAKPEKDQK
     YQGKDITLAS TGAIVVMDPR NGEVLASASY PSYDPNWFMQ GLTKEQSEYL FGEAATDTTP
     LRNKAVSARY APGSIFKPLT GVAGVSEGVV GIDEIVNDHG DSGYYYFYSV DENGKTVVQK
     QGAPRCWSYR NHTAHANINL TQALTFSCNY YFCEIANRMG IDTLNKWMGK FGLDTKTNIE
     LTGETAGVGG GQKVLFDNEL TDSNGELNIT GQKTSLPILI YNRIRERLRE YVTRRSMEID
     EQAIRKCALK LMMLQDGKGL DGKGPDIRKI LSDELGIPEG YSITQSWTSE IVTLLNEIQW
     KPTQTIRAGF GQGTTLVTPM AIARYVSAIA NEGTVYDANI VERIVDQNGN LIEDKNAAVY
     ETIGEDTAEW DALWAAVKQG MAGVVSAEDH GTAGGKFSQE FTEKYLDRIA GKTGSAQVGT
     TSIDIENTSW FVSYTPREGE AELVIVICVP SGYAGVWSVS AAEEIYTYYF NKQDSAAPET
     LVDIGGIAP
//