ID R5B6P8_9CLOT Unreviewed; 552 AA.
AC R5B6P8;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alani ne ligase {ECO:0000313|EMBL:CCX50948.1};
GN ORFNames=BN545_00029 {ECO:0000313|EMBL:CCX50948.1};
OS Clostridium sp. CAG:226.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1262781 {ECO:0000313|EMBL:CCX50948.1, ECO:0000313|Proteomes:UP000018047};
RN [1] {ECO:0000313|EMBL:CCX50948.1, ECO:0000313|Proteomes:UP000018047}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:226 {ECO:0000313|Proteomes:UP000018047};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCX50948.1}.
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DR EMBL; CAWN010000223; CCX50948.1; -; Genomic_DNA.
DR AlphaFoldDB; R5B6P8; -.
DR Proteomes; UP000018047; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR Gene3D; 3.90.190.20; Mur ligase, C-terminal domain; 1.
DR Gene3D; 3.40.1190.10; Mur-like, catalytic domain; 1.
DR InterPro; IPR036565; Mur-like_cat_sf.
DR InterPro; IPR004101; Mur_ligase_C.
DR InterPro; IPR036615; Mur_ligase_C_dom_sf.
DR InterPro; IPR013221; Mur_ligase_cen.
DR PANTHER; PTHR43024; UDP-N-ACETYLMURAMOYL-TRIPEPTIDE--D-ALANYL-D-ALANINE LIGASE; 1.
DR PANTHER; PTHR43024:SF1; UDP-N-ACETYLMURAMOYL-TRIPEPTIDE--D-ALANYL-D-ALANINE LIGASE; 1.
DR Pfam; PF02875; Mur_ligase_C; 1.
DR Pfam; PF08245; Mur_ligase_M; 1.
DR SUPFAM; SSF53623; MurD-like peptide ligases, catalytic domain; 1.
DR SUPFAM; SSF53244; MurD-like peptide ligases, peptide-binding domain; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Ligase {ECO:0000313|EMBL:CCX50948.1}; Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000018047};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..34
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 81..103
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 123..144
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 209..400
FT /note="Mur ligase central"
FT /evidence="ECO:0000259|Pfam:PF08245"
FT DOMAIN 420..498
FT /note="Mur ligase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02875"
SQ SEQUENCE 552 AA; 60384 MW; 0883E2D365DC3F54 CRC64;
MGNELVSSIL SYALSVFMGA AVVAASVPLV HILQLESYQG KMYLKWLMKH IGSDFVPSFV
AGVAALALRA GYVLIGGTNP LIGRICYYLA DIVYIAMLAV IYFSYEKKPH VKPIAYTGRV
KRLLAAEFIL ATLFSAMFFS PLLWDKYGLA WIVYMPPMII RYLPGLILPL FVFICYIITY
PIEEGVKQWY LNDAKRMLKE HEGLIRIGIT GSYGKTGVKY ALSEILSRKY NTLITPGSYN
TPMGVTRTVR EHLSDANEAF VAEMGARYVG DIKTLCKLVH PQYGIITAVG KQHLETFKSL
DNIINTKAEL ISGLEEGGCC FLNGDDENCR RIYKEYASVR KLYLYGTEGD DLYMKAESIK
VTSEGSTFDL VAEDGAKVSC TAQLLGRHNI VNLTAAAALA YKLGMGLDEI AAGIAGARPV
EHRLQLIPGA VTVIDDAFNS NPVGSKEALK VLGSFEGKKR VVVTPGMVEL GAEEAELNRE
FGRCIAENAD VAIIIGKAHA DPICEGIAER GFDPANLVRV ASLKEATEKL AQYGGAGSVV
LFENDLPDNY NE
//
