ID R5GYM9_9FIRM Unreviewed; 629 AA.
AC R5GYM9;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=BN782_00663 {ECO:0000313|EMBL:CCY20894.1};
OS Eubacterium sp. CAG:786.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=1262893 {ECO:0000313|EMBL:CCY20894.1, ECO:0000313|Proteomes:UP000018127};
RN [1] {ECO:0000313|EMBL:CCY20894.1, ECO:0000313|Proteomes:UP000018127}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:786 {ECO:0000313|Proteomes:UP000018127};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCY20894.1}.
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DR EMBL; CAXS010000367; CCY20894.1; -; Genomic_DNA.
DR AlphaFoldDB; R5GYM9; -.
DR STRING; 1262893.BN782_00663; -.
DR Proteomes; UP000018127; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
FT DOMAIN 161..541
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 629 AA; 69628 MW; B8D1CAA71C3F985A CRC64;
MELSLVDKNY AYAGELGALY SQAQTTFRVW SPLAEKAAVK LYQNAGSRIP YCVCKMQRSG
NSWEATIPGD LHGVFYTYEF TSSGKSRETI DIYARSAGAN GVRGMVVDLS RTDPDGWEND
RPVKLESYTD AVIYELHVRD FSSDESGNFK LRGKFGAFCE KGVTNGFSDA IGLDHIASLG
ITHIHLLPVF DYQTVDETDP EAGFNWGYDP LNYNIPEGSY TTDPDNGTQR VKQFKELIHA
VHKKGIGVIM DVVYNHTYST ENSPFTKTFP GYYYRHNADG SLSDGSACGN EFASERVMAS
RFIVDSLCYL AEEYKLDGFR FDLMGLLDIQ TLNKAAEKLR RINPSVILYG EGWTGGASPL
DEGLRAMKKN AVELPQFAMF SDDFRDGVKG SVFEDEGCGY VNGNAEELSE LMKSVISAGV
FRSDVERPES ECWTDTPQQA VNYVEAHDNL TLFDKLRVSM PSASEEACIA ADKLAAALVF
LSQGIPFMQA GQELLRSKPA PGGGFVHDSY NSPDSVNGIK WNDVTIRRSV MEYYRGLIAI
RKRFPEFRLR TSEEIRTLVK YEDIGSGALA VHYGEKFLLL INPGEAPLEC PLPGVADIYA
DSKKADTQPL YQAEGVVSVK PCSIILAGF
//
