ID R5IV06_9CLOT Unreviewed; 617 AA.
AC R5IV06;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=Peptidase S8 {ECO:0008006|Google:ProtNLM};
GN ORFNames=BN757_01291 {ECO:0000313|EMBL:CCY40627.1};
OS Clostridium sp. CAG:7.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1262832 {ECO:0000313|EMBL:CCY40627.1, ECO:0000313|Proteomes:UP000018268};
RN [1] {ECO:0000313|EMBL:CCY40627.1, ECO:0000313|Proteomes:UP000018268}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:7 {ECO:0000313|Proteomes:UP000018268};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000256|PROSITE-
CC ProRule:PRU01240, ECO:0000256|RuleBase:RU003355}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCY40627.1}.
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DR EMBL; CAYE010000020; CCY40627.1; -; Genomic_DNA.
DR AlphaFoldDB; R5IV06; -.
DR STRING; 1262832.BN757_01291; -.
DR Proteomes; UP000018268; Unassembled WGS sequence.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd07478; Peptidases_S8_CspA-like; 1.
DR Gene3D; 2.60.120.1290; -; 1.
DR Gene3D; 3.30.70.2980; -; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR InterPro; IPR041365; CspB_prodomain.
DR InterPro; IPR034045; Pep_S8_CspA-like.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR PANTHER; PTHR43399; SUBTILISIN-RELATED; 1.
DR PANTHER; PTHR43399:SF4; TK-SUBTILISIN; 1.
DR Pfam; PF18425; CspB_prodomain; 1.
DR Pfam; PF00082; Peptidase_S8; 2.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01240};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000018268};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW ProRule:PRU01240}.
FT DOMAIN 9..96
FT /note="Csp protease B prodomain"
FT /evidence="ECO:0000259|Pfam:PF18425"
FT DOMAIN 155..338
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT DOMAIN 470..601
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT ACT_SITE 164
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 231
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 545
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 617 AA; 67109 MW; B88C141B4B19061D CRC64;
MAIAANTDQK RENLLNLSLS VSEEERMRSE SLETGFDREE RTWELIVKYF GDLSGLRERG
IQVRELLSGY GILKVPEYLV DFVTNLPEIE YIEKPKRLFF AVNQARAASC LLDVQQGIGG
ENANSDPLEN IFGMDGGTVT ERVSAETKLP TDLTGKGVLI AIIDSGIDYL HPDFQNPDGT
SRILYLWDQG RDIVYTKEEI NEALSAYREG SGGRNRAAAL QIVPSADTSG HGTAVAAIAA
GNGRGSSGLY RGVACESELM VVKLGIPLAD NFPRTTQLME AVDFVLRTAR QLGRPVAVNL
SFGNTYGSHD GTSLLETFLD DMTGYGRNVI VAGTGNEGAG AGHTGGLLEM GREQVIELSV
SAFENSFGVQ LWKSYADIFS ISLRSPSGAI MGTLQENLEK QRFRSENTEI LSYYGKPSPY
SMAQEVYFDF IPAGDYIDSG IWQFILTPER IITGQFDLWL PSKAALNNDT RFLKSTPETT
LTIPSTARKI ISAGAYDFRT RAYADFSGRG FTRQTNQVKP DLVAPGVNII TAGNRGGYEA
VTGTSFAAPF VTGAAALLMQ WGIINGNDPF LYGEKVKACL IRGARHLPGY DIWPNARVGY
GSLCVSESLP LRSGGEG
//
