ID R5V6N7_9FIRM Unreviewed; 463 AA.
AC R5V6N7;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:CCZ83784.1};
GN ORFNames=BN566_01519 {ECO:0000313|EMBL:CCZ83784.1};
OS Ruminococcus sp. CAG:254.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=1262953 {ECO:0000313|EMBL:CCZ83784.1, ECO:0000313|Proteomes:UP000018181};
RN [1] {ECO:0000313|EMBL:CCZ83784.1, ECO:0000313|Proteomes:UP000018181}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:254 {ECO:0000313|Proteomes:UP000018181};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the radical SAM superfamily. Anaerobic
CC sulfatase-maturating enzyme family. {ECO:0000256|ARBA:ARBA00023601}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCZ83784.1}.
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DR EMBL; CBAR010000161; CCZ83784.1; -; Genomic_DNA.
DR AlphaFoldDB; R5V6N7; -.
DR STRING; 1262953.BN566_01519; -.
DR Proteomes; UP000018181; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd21124; SPASM_CteB-like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR000385; MoaA_NifB_PqqE_Fe-S-bd_CS.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR024025; SCIFF_rSAM_maturase.
DR InterPro; IPR047602; SPASM_CteB-like.
DR InterPro; IPR023867; Sulphatase_maturase_rSAM.
DR NCBIfam; TIGR04085; rSAM_more_4Fe4S; 1.
DR NCBIfam; TIGR03974; rSAM_six_Cys; 1.
DR PANTHER; PTHR43273; ANAEROBIC SULFATASE-MATURATING ENZYME HOMOLOG ASLB-RELATED; 1.
DR PANTHER; PTHR43273:SF8; RADICAL SAM PROTEIN-RELATED; 1.
DR Pfam; PF13353; Fer4_12; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF13186; SPASM; 1.
DR SFLD; SFLDG01072; dehydrogenase_like; 1.
DR SFLD; SFLDG01384; thioether_bond_formation_requi; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS01305; MOAA_NIFB_PQQE; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000018181};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 91..321
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 463 AA; 52916 MW; AB47588F8991DE8A CRC64;
MIHKYKLHGY NVVLDVNSGG VHLVDDLTYD MLDNVAPPFA EVCPPEVVEK LSAFYQEADI
LSCYDEVLEA YNEQILFSED DYEKYADCSV ASPIKAMCLH IAHDCNLRCE YCFASTGDFG
EGRKLMPLET GKQAIDFLLK HSANRKNLEL DFFGGEPLMN FEVVKQVVAY ARSKEQEYGK
HFRFTITTNG MLLNDDNIDF INKEMSNVVL SIDGRKAVND RMRKRVDGKG SYDIILPKYQ
RLVAQRGDKE YYVRGTYTKY NLDFSNDVFD LLEHGFDQIS VEPVMADEND PYAITEADLP
AIFKEYEVLM NKILDYEKTG KKFNFFHFML DLDQGPCAIK RLRGCGCGNE YVAITPDGDI
YPCHQFVGME EWKMGNLEEG TFDQAKKADF AKAHIYTKPD CRECWARFYC SGGCNANNYQ
YAGDIRKAHK LSCEIEKKRL ECAIVMKAIR MEQAEQAAAE SNS
//
