ID R5Y9Q3_9FIRM Unreviewed; 817 AA.
AC R5Y9Q3;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=beta-mannosidase {ECO:0000256|ARBA:ARBA00012754};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
GN ORFNames=BN695_01568 {ECO:0000313|EMBL:CDA13347.1};
OS Anaerotruncus sp. CAG:528.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Anaerotruncus.
OX NCBI_TaxID=1262700 {ECO:0000313|EMBL:CDA13347.1, ECO:0000313|Proteomes:UP000018024};
RN [1] {ECO:0000313|EMBL:CDA13347.1, ECO:0000313|Proteomes:UP000018024}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:528 {ECO:0000313|Proteomes:UP000018024};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDA13347.1}.
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DR EMBL; CBBE010000059; CDA13347.1; -; Genomic_DNA.
DR AlphaFoldDB; R5Y9Q3; -.
DR STRING; 1262700.BN695_01568; -.
DR Proteomes; UP000018024; Unassembled WGS sequence.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023001};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:CDA13347.1};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023001};
KW Reference proteome {ECO:0000313|Proteomes:UP000018024}.
FT DOMAIN 59..127
FT /note="Glycosyl hydrolases family 2 sugar binding"
FT /evidence="ECO:0000259|Pfam:PF02837"
FT DOMAIN 627..704
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 718..784
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 817 AA; 93060 MW; BFEF85E5332C7FE4 CRC64;
MERMSLAGKW ILHMDKQDRD YEADVPCSDY GTLLKNGAID DPFYKTNEHK CLFIAETGKS
FKRSFKISQE QLEYRKALLR CQMLDTLAQI YINGQMADIS SNAYVCSETN ITDYLHEGEN
TIEIHFLSPI EYVKSKQSEA PLPPNANGID GVPYIRKPAC HFGWDWGINM PVSGIFGNIE
ILFYNNEIRD FWVRQEHSEN GEVKLYIKAN KALDSSGQII TPSGESIAFG IEKGNACVTI
KNPELWQTRE LSGKEKQPLY TVCVAGIKKR IGLRTIVLDR SEDEYGSNFR FILNGVPIFA
KGANVIPPDA MADRIGKKTA EKIVSDAVHA NFNMLRIWGG GYYGSDELYD LCDEYGILVW
QDFMFACLMY PFYDEVFLKN VLDEVKYNVR RISSHPSLAL WCGNNEIEFM FQHLPSTLKI
VKWYKEFFYE ILPGELRRYD KDTPYIETSP IGSGFRKDIT ADKCGDTHMW HVWHGSKDLS
YYGKRYTRFC SEYGMESFPS EDCILQFATQ KDMQLKSDVM QSHQKCASGN DKMLFYLYEK
FNKPAKFADL IYLTGLTQAE CVANGAEHWR RNKGRCNGAL WWQMNDCWGA PSWSSVDYFG
KWKPLMYAGK RIFAPITISL RQADGGIELY VLNGTQKNEE YTAVITFKDF TGKELRSKSL
LIKSHAGESQ KAFYISAGGL KKASCFAQAE LLKGGKTVLS RTAVLLPERK LKLQHAKITL
YLSGNEMTLE SNTFARSVFV DIKGESAPLS DNCFDLVPGE KKTIRLEKDC KIDPKSISVK
CVNNITYAGG DAERIRFRMA FSAQPMNIAN KIYYSIS
//
