ID R6BWG6_9CLOT Unreviewed; 457 AA.
AC R6BWG6;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE SubName: Full=MiaB-like tRNA modifying enzyme {ECO:0000313|EMBL:CDA68483.1};
GN ORFNames=BN687_00491 {ECO:0000313|EMBL:CDA68483.1};
OS Clostridium sp. CAG:510.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1262816 {ECO:0000313|EMBL:CDA68483.1, ECO:0000313|Proteomes:UP000018120};
RN [1] {ECO:0000313|EMBL:CDA68483.1, ECO:0000313|Proteomes:UP000018120}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:510 {ECO:0000313|Proteomes:UP000018120};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDA68483.1}.
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DR EMBL; CBCI010000053; CDA68483.1; -; Genomic_DNA.
DR AlphaFoldDB; R6BWG6; -.
DR STRING; 1262816.BN687_00491; -.
DR Proteomes; UP000018120; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0035598; F:N6-threonylcarbomyladenosine methylthiotransferase activity; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.40.50.12160; Methylthiotransferase, N-terminal domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR005839; Methylthiotransferase.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR013848; Methylthiotransferase_N.
DR InterPro; IPR038135; Methylthiotransferase_N_sf.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR034557; ThrcA_tRNA_MEthiotransferase.
DR NCBIfam; TIGR00089; MiaB/RimO family radical SAM methylthiotransferase; 1.
DR PANTHER; PTHR43020; CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1; 1.
DR PANTHER; PTHR43020:SF2; MITOCHONDRIAL TRNA METHYLTHIOTRANSFERASE CDK5RAP1; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF00919; UPF0004; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01061; methylthiotransferase; 1.
DR SFLD; SFLDF00295; threonylcarbamoyladenosine_tRN; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51449; MTTASE_N; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000018120};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2..114
FT /note="MTTase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51449"
FT DOMAIN 140..392
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 457 AA; 52526 MW; 41B5A320EE853FDC CRC64;
MKRAALHNLG CKVNAYELDI MQQNLQEKGY TIVSFDEMAD IYIVNTCTVT NIADRKSRQM
LHQAKKRNPE AVVVAVGCYV QTADEEALRE QGIDLAIGNN RKKDLIPILE KFLAEHKQEE
DAIIDVNAPC DYEEMTLKHT AEHTRAYIKI QDGCNQFCSY CIIPYARGRV RSRRYEDIRR
EMEGLAQSGY KEVVLTGIHI SSYGIDFSKE AWKQGETEVK VKDDGRRDYA GESKLIDLLE
KLQDIEGIER IRIGSLEPRI MTEKNVKRLA SIEKLCPHFH LSLQSGCDET LKRMNRHYTT
GEYLEGTKRL REAFVHPAIT TDVIVGFPGE TEEEFAKTCG FLREADLYEM HIFKYSKRKG
TVAAGMPDQI PGPVQTKRSA ELLAMEKELS KRYREYYIGK EIPVLFEEEK EIGGIRYQMG
HSREYVKVAK KTEENLSNRI LSGKIVDFLQ EDILLMQ
//
