ID R6CK00_9BACE Unreviewed; 1190 AA.
AC R6CK00;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=beta-galactosidase {ECO:0000256|ARBA:ARBA00012756};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
DE AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230};
GN ORFNames=BN697_02329 {ECO:0000313|EMBL:CDA75970.1};
OS Bacteroides sp. CAG:530.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=1262741 {ECO:0000313|EMBL:CDA75970.1, ECO:0000313|Proteomes:UP000018392};
RN [1] {ECO:0000313|EMBL:CDA75970.1, ECO:0000313|Proteomes:UP000018392}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:530 {ECO:0000313|Proteomes:UP000018392};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDA75970.1}.
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DR EMBL; CBCL010000087; CDA75970.1; -; Genomic_DNA.
DR AlphaFoldDB; R6CK00; -.
DR Proteomes; UP000018392; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.80.10.50; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR023232; Glyco_hydro_2_AS.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF16353; LacZ_4; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 1.
DR PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000018392};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..1190
FT /note="beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004414554"
FT DOMAIN 930..1187
FT /note="Beta galactosidase small chain/"
FT /evidence="ECO:0000259|SMART:SM01038"
SQ SEQUENCE 1190 AA; 134661 MW; 694889A1D2125C2F CRC64;
MKKCFLYLLV MSLCMLAFGE AYAQNARFSP VNNPDKAIGY ESGKVIVVLK KTDNNDKLQQ
WTVSKLAGSV RLINPYTNKA VHVRADYLLG IAENNGSDES QLWTIRKKGK YVQLIPTNSP
DLILVSTSGK LQLQNSAKAA EKTTLFDMKE FALEASETSL NGEKREKVYW EDETRFEENK
EAGHATFIPY PSEQAMMADK AFYNTPWVDT KSSKVMSLNG KWAFRFVSEP SQRPLDFYKD
DYDVSSWDAI PVPSNWEMHG YDRPIYCNVE YPHSNTPPFI NARKGFNDGG KNYGINPVGS
YVRFFDMPQN WDKQRTFIHF GGIYSAALVY MNGEYVGYTQ GANNVSEFDV TRYLRPGKNR
LAVQVFRWSD GSYLECQDMF RMSGIFRDVY LFNTPKVSIF DHYITSQLDA SSDYRNGKMN
VHFTIDNRDG LKGSRNFVVK LFAPDGKLVA ESEKALQFDS KTLRLKLDVP FSVQSVMPWT
AETPNLYTVH VIQRNGANDE QAFSTKYGFR DIAIRGSLVY INGKRVFFKG VNRHDSHPLY
GRAVTTESML QDVLLMKRNN INTIRTSHYP NAAKMYAMFD YYGLYTMDEA DLEDHANQSI
SDMESWIPSF VDRITRLVTR DRNHPSVIFW SLGNEAGGGA NFQFCYDEAK RLDTRPVHYE
GTRDGKPYGG NRFSDLYSKM YPGMSWMNKY VNSFDKPMFI CEYAHAMGNA IGNLKEYWQS
IESSTTTIGG AIWDWVDQGI YEPKEIKKGI YRLHTGYDFP GPHQGNFCCN GILLPTREET
PKLKEVKAAH QFMRMELTGM DDAKGEASVK VDNTYDFLTG KEFYLRYEVL VDGKKVSVDS
LSLPDIPSEA SENMVLGLKN VDMQQLKADG KEVMLNMDIC LKHAATWAEA GHSVAQQQIE
LAARKPMSAY VMPKGKYKKI TIAQDDNTLV VGNDRITAIF CRKSGKLLSL DMNGQNVISD
KLGMEYSNHR WIENDRFTKT ENGLDAEGTL LVKKNGKNVE VTAQRGGSLC ATDMVYTFMP
DGTLDMEVQF SPKAKNLRRA GLLCGVNAEL GNVEYYAYGP WENHTDRKDG CMVGIFKTTV
DDMLVPYVKP QSTGNREGLR CLKLTDANGK GVSITTEGNV SFSALRYTDE DLMNANHIWE
LQARPFIALH LDAMLTGVGN ASCGHDVGPL PMYSVPESPL SYKLRIQSVR
//
