ID   R6L0S4_9FIRM            Unreviewed;       539 AA.
AC   R6L0S4;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   24-JAN-2024, entry version 26.
DE   SubName: Full=Glycoside Hydrolase Family 43 candidate b-xylosidase or a-L-arabinofuranosidase {ECO:0000313|EMBL:CDB67927.1};
GN   ORFNames=BN564_01581 {ECO:0000313|EMBL:CDB67927.1};
OS   Eubacterium sp. CAG:252.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC   Eubacterium.
OX   NCBI_TaxID=1262887 {ECO:0000313|EMBL:CDB67927.1, ECO:0000313|Proteomes:UP000017970};
RN   [1] {ECO:0000313|EMBL:CDB67927.1, ECO:0000313|Proteomes:UP000017970}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:252 {ECO:0000313|Proteomes:UP000017970};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDB67927.1}.
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DR   EMBL; CBEA010000073; CDB67927.1; -; Genomic_DNA.
DR   AlphaFoldDB; R6L0S4; -.
DR   Proteomes; UP000017970; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd18617; GH43_XynB-like; 1.
DR   Gene3D; 2.60.120.200; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR041542; GH43_C2.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR   PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR   Pfam; PF17851; GH43_C2; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR   SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187}.
FT   DOMAIN          330..519
FT                   /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT                   /evidence="ECO:0000259|Pfam:PF17851"
FT   ACT_SITE        14
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        174
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            120
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   539 AA;  60524 MW;  04D80ABBB43DD221 CRC64;
     MLFKNPIIPG YNPDPSICRV GDDFYLVNST FEFFPGVPIY HSRNLVNWEL KGYCLDRRSQ
     LELEGCRNSG GIYAPTIRFH NGMFYMITTN VTDKGNFVVH SDSVDGDWSE PAWIDQGGID
     PSLFWDDDDK CYYCSTGILD GVRGIVAFEI NPMTGEILSD KKLISEGCGG QCAEGPHIYK
     KDGMYYLMIA EGGTEYAHRE TIQRAENVWG PYTPCPHNPI ISHKEYKKSE IQATGHADLI
     EDANGNWWLV FLGIRRFSHA LLHNLGRETF LAPVKWEDGW PVVGYNGNGT IELVMDAPLP
     GLDYEESKAN IHTDDKTGKP MLYADHSVSI DFADEKLDKR LQFTRNPDMS HYIYDSADRK
     LTLKGTDISL NEPGKSPTIL SFKQPEFTTT LKAVVDIGAT TAKRAGIAAY YNNDYHYEIY
     IGNDDNGKYI GFYKHIHDMG AELIHIPVSK DEENMKLLVK IDTDREKYTF SYAIADTDNL
     NARVAFRQAG SGLNAGLSTE GTRTMTFTGT LFSLFAEDGD GVFDTGVQLS INPDEDYKL
//