ID R6QR62_9BACT Unreviewed; 524 AA.
AC R6QR62;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=Exo-poly-alpha-D-galacturonosidase {ECO:0000313|EMBL:CDC24427.1};
GN ORFNames=BN637_00072 {ECO:0000313|EMBL:CDC24427.1};
OS Prevotella sp. CAG:386.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Prevotellaceae;
OC Prevotella.
OX NCBI_TaxID=1262925 {ECO:0000313|EMBL:CDC24427.1, ECO:0000313|Proteomes:UP000018046};
RN [1] {ECO:0000313|EMBL:CDC24427.1, ECO:0000313|Proteomes:UP000018046}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:386 {ECO:0000313|Proteomes:UP000018046};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-
CC galactosidic residues, producing free D-galactose.; EC=3.2.1.n1;
CC Evidence={ECO:0000256|ARBA:ARBA00001271};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family.
CC {ECO:0000256|ARBA:ARBA00008834, ECO:0000256|RuleBase:RU361169}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDC24427.1}.
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DR EMBL; CBEZ010000035; CDC24427.1; -; Genomic_DNA.
DR AlphaFoldDB; R6QR62; -.
DR STRING; 1262925.BN637_00072; -.
DR Proteomes; UP000018046; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0004650; F:polygalacturonase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.160.20.10; Single-stranded right-handed beta-helix, Pectin lyase-like; 1.
DR InterPro; IPR000743; Glyco_hydro_28.
DR InterPro; IPR006626; PbH1.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR PANTHER; PTHR31339; PECTIN LYASE-RELATED; 1.
DR PANTHER; PTHR31339:SF9; PLASMIN AND FIBRONECTIN-BINDING PROTEIN A; 1.
DR Pfam; PF00295; Glyco_hydro_28; 1.
DR SMART; SM00710; PbH1; 5.
DR SUPFAM; SSF51126; Pectin lyase-like; 1.
DR PROSITE; PS00502; POLYGALACTURONASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361169};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361169};
KW Reference proteome {ECO:0000313|Proteomes:UP000018046};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..524
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004416719"
SQ SEQUENCE 524 AA; 57734 MW; 45E765B0EEEF00A0 CRC64;
MKKLFSLVCT CLLAMSMQAA ETDKYDALYE NLPFQMEKVT RPQFPANEVN LKDFGAVGDG
STLCTEAFSK AIEALSQKGG GKLIVPQGVW FTGPITLKSN INLHIEKGGI ILFSPDDALY
PFVKTSFEGL DTRRCQSPIS GNGLTNVAIT GQGAIDGNGE YWRPLKKQKV TDAQWKAITS
RGGAYKRKDY WFPSEGALKA DNSANMNVPA EPTSEEGWNE IKRFLRPVMV SLVNCKNVWF
NGVIFQNSPA WNIHPLMCEN VLIEDVLVRN PAYAQNGDGL DLESCKNALI VNSTFDVGDD
GICIKSGKDA DGRKRGIPCE NVIVDGCTVF KGHGGFVVGS EMSGGVKNVM VTNCQFLGTD
VGLRFKSTRG RGGVVENIFI KNMSMFDIQT DVITFDLYYG GKSAVEVLND GDEAKNQKVE
KFKVNETTPC FRNIDIDHVI CRTARRAAYF NGLPEMPVQN ISIKDLEVNN AEQGIVINRT
ENVKLENIKV TAKTHTFDAK NSKNVSVNSK TYKKIDEKGI TLDF
//
