ID R7B2S4_9CLOT Unreviewed; 391 AA.
AC R7B2S4;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE SubName: Full=Restriction endonuclease {ECO:0000313|EMBL:CDD58346.1};
GN ORFNames=BN653_01953 {ECO:0000313|EMBL:CDD58346.1};
OS Clostridium sp. CAG:43.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1262805 {ECO:0000313|EMBL:CDD58346.1, ECO:0000313|Proteomes:UP000018284};
RN [1] {ECO:0000313|EMBL:CDD58346.1, ECO:0000313|Proteomes:UP000018284}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:43 {ECO:0000313|Proteomes:UP000018284};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDD58346.1}.
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DR EMBL; CBHG010000332; CDD58346.1; -; Genomic_DNA.
DR AlphaFoldDB; R7B2S4; -.
DR STRING; 1262805.BN653_01953; -.
DR Proteomes; UP000018284; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR Gene3D; 3.40.91.80; -; 1.
DR Gene3D; 2.40.330.10; DNA-binding pseudobarrel domain; 1.
DR InterPro; IPR015300; DNA-bd_pseudobarrel_sf.
DR InterPro; IPR038365; EcoRII_C_sf.
DR InterPro; IPR023372; Rest_endonuc_II_EcoRII_N.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR015109; Restrct_endonuc_II_EcoRII_C.
DR Pfam; PF09019; EcoRII-C; 1.
DR Pfam; PF09217; EcoRII-N; 1.
DR SUPFAM; SSF101936; DNA-binding pseudobarrel domain; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
PE 4: Predicted;
KW Endonuclease {ECO:0000313|EMBL:CDD58346.1};
KW Hydrolase {ECO:0000313|EMBL:CDD58346.1};
KW Nuclease {ECO:0000313|EMBL:CDD58346.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000018284}.
FT DOMAIN 20..116
FT /note="Restriction endonuclease type II EcoRII N-terminal"
FT /evidence="ECO:0000259|Pfam:PF09217"
FT DOMAIN 217..382
FT /note="Restriction endonuclease type II EcoRII C-terminal"
FT /evidence="ECO:0000259|Pfam:PF09019"
SQ SEQUENCE 391 AA; 44562 MW; 66BB5890D0F64B3F CRC64;
MITGYAGQAI KSVLNSEKGL CKFLSANDTG ATGGHQSGIL ISKSAVEMMF SKQELEQAGI
PKRTVKVRWQ DDFETESCFT YYESKNELRI TRFGRGFPFL KPEQTGALFV FTQQTDFDYC
GYFLETDEEI EEFLDTFGIS PTETNSLIDT GKISSETQEK LAIQEFISGL DLDFPASDVM
SAAARTIEEK AYDHVEYILT NPDEKIISWT NMEYTLFRAL EYARYGDIIT KGFQSVDEFV
RVANVVLNRR KSRAGKSLEH HLSAIFDGNG IEYSSQAVTE GNKKPDFLFP SQEAYHDYDF
STDYIISLAA KTTCKDRWRQ VLNEADRLRD KPKYLCTLQQ GISGAQMDEM QAENVILVVP
KPYIATYPKD RRDRIWTLAR FVQYVKTIEG K
//
