ID   R7F590_9CLOT            Unreviewed;       671 AA.
AC   R7F590;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   24-JAN-2024, entry version 30.
DE   SubName: Full=Penicillin-binding protein 2 {ECO:0000313|EMBL:CDE09801.1};
GN   ORFNames=BN623_01162 {ECO:0000313|EMBL:CDE09801.1};
OS   Clostridium sp. CAG:354.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1262799 {ECO:0000313|EMBL:CDE09801.1, ECO:0000313|Proteomes:UP000018313};
RN   [1] {ECO:0000313|EMBL:CDE09801.1, ECO:0000313|Proteomes:UP000018313}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:354 {ECO:0000313|Proteomes:UP000018313};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the transpeptidase family.
CC       {ECO:0000256|ARBA:ARBA00007171}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDE09801.1}.
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DR   EMBL; CBIG010000003; CDE09801.1; -; Genomic_DNA.
DR   AlphaFoldDB; R7F590; -.
DR   STRING; 1262799.BN623_01162; -.
DR   Proteomes; UP000018313; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:InterPro.
DR   Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR   Gene3D; 3.90.1310.10; Penicillin-binding protein 2a (Domain 2); 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR007887; MecA_N.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR005311; PBP_dimer.
DR   InterPro; IPR036138; PBP_dimer_sf.
DR   InterPro; IPR001460; PCN-bd_Tpept.
DR   PANTHER; PTHR30627:SF25; PENICILLIN-BINDING PROTEIN 3; 1.
DR   PANTHER; PTHR30627; PEPTIDOGLYCAN D,D-TRANSPEPTIDASE; 1.
DR   Pfam; PF05223; MecA_N; 1.
DR   Pfam; PF03717; PBP_dimer; 1.
DR   Pfam; PF00905; Transpeptidase; 1.
DR   SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR   SUPFAM; SSF54427; NTF2-like; 1.
DR   SUPFAM; SSF56519; Penicillin binding protein dimerisation domain; 1.
PE   3: Inferred from homology;
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018313};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          32..146
FT                   /note="NTF2-like N-terminal transpeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF05223"
FT   DOMAIN          153..321
FT                   /note="Penicillin-binding protein dimerisation"
FT                   /evidence="ECO:0000259|Pfam:PF03717"
FT   DOMAIN          354..661
FT                   /note="Penicillin-binding protein transpeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF00905"
SQ   SEQUENCE   671 AA;  73854 MW;  4E5B920886508950 CRC64;
     MKNKKVLIGT LIILVIVIVA IVAFFMLNKS EKPEDALNNY FSKIGEQKYE EAYDLISSSS
     KDKTAKETFV NKNDSIYSGI DMTNLSTEIT NVEKADDGEK ITYTQKMNTS AGEVSFTNTV
     TLVKEDKEYK LNWSNSMIFP ELGDNDKIRV KTLEAERGNI YDRNNVLLAG KGNISSIGLV
     PGKMSANKDE DIQKLSTILG VSVDSINNKL NMSYVKDDTF VPIKNVATTE SQIKEQVLTI
     PGVKITTESS RVYTLGREAS QLIGYVQAIS AEELESNAGK GYNSNSLIGK AGIEKAYEDT
     LRGKDGKEIY IENEDGDKVK TIAKQELENG KDVKLTIDAT TQKQVYDSLE ENKGAYVAMD
     SKTGEILALV STPSYDSNDF VLGMSTDEWN SLNQDENKPL LNRFTGTWSP GSTFKPVTGA
     IGITEGKINP NEDFGRSGLS WQKDSSWGNY MVTTLTPYNE PANMQNALIR SDNIYFAKAA
     LKIGYDAFME GLNKLGFNED ISFELSTSKS TYDTDGKIDD EVQLADSGYG QGQILVNPIH
     MASIYSAFVN NGNMIKPTII YEENKQPEYL KENAISEEAA NTIKEDLIQV VENPNGTAHD
     AKIDGLTIGA KTGTAELKKS KDEEGEVIGW FNAFTADETS AKQLVVVSMV EDANSVGGSH
     YLFPKVTQIF K
//