ID R7KLK3_9BURK Unreviewed; 444 AA.
AC R7KLK3;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 28-MAR-2018, entry version 28.
DE RecName: Full=Exodeoxyribonuclease 7 large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
DE EC=3.1.11.6 {ECO:0000256|HAMAP-Rule:MF_00378};
DE AltName: Full=Exodeoxyribonuclease VII large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
DE Short=Exonuclease VII large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
GN Name=xseA {ECO:0000256|HAMAP-Rule:MF_00378};
GN ORFNames=BN692_00210 {ECO:0000313|EMBL:CDE77269.1};
OS Sutterella sp. CAG:521.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Sutterellaceae; Sutterella; environmental samples.
OX NCBI_TaxID=1262977 {ECO:0000313|EMBL:CDE77269.1, ECO:0000313|Proteomes:UP000018289};
RN [1] {ECO:0000313|EMBL:CDE77269.1, ECO:0000313|Proteomes:UP000018289}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:521 {ECO:0000313|Proteomes:UP000018289};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J.,
RA Sunagawa S., Plichta D., Gautier L., Le Chatelier E., Peletier E.,
RA Bonde I., Nielsen T., Manichanh C., Arumugam M., Batto J.,
RA Santos M.B.Q.D., Blom N., Borruel N., Burgdorf K.S., Boumezbeur F.,
RA Casellas F., Dore J., Guarner F., Hansen T., Hildebrand F., Kaas R.S.,
RA Kennedy S., Kristiansen K., Kultima J.R., Leonard P., Levenez F.,
RA Lund O., Moumen B., Le Paslier D., Pons N., Pedersen O., Prifti E.,
RA Qin J., Raes J., Tap J., Tims S., Ussery D.W., Yamada T.,
RA MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P., Wang J.,
RA Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units
RT of genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Bidirectionally degrades single-stranded DNA into large
CC acid-insoluble oligonucleotides, which are then degraded further
CC into small acid-soluble oligonucleotides. {ECO:0000256|HAMAP-
CC Rule:MF_00378, ECO:0000256|SAAS:SAAS00723532}.
CC -!- CATALYTIC ACTIVITY: Exonucleolytic cleavage in either 5'- to
CC 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.
CC {ECO:0000256|HAMAP-Rule:MF_00378, ECO:0000256|RuleBase:RU004355,
CC ECO:0000256|SAAS:SAAS00723505}.
CC -!- SUBUNIT: Heterooligomer composed of large and small subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00378, ECO:0000256|SAAS:SAAS00984457}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00378,
CC ECO:0000256|RuleBase:RU004355, ECO:0000256|SAAS:SAAS00723552}.
CC -!- SIMILARITY: Belongs to the XseA family. {ECO:0000256|HAMAP-
CC Rule:MF_00378, ECO:0000256|RuleBase:RU004355,
CC ECO:0000256|SAAS:SAAS00723548}.
CC -!- CAUTION: The sequence shown here is derived from an
CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC preliminary data. {ECO:0000313|EMBL:CDE77269.1}.
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DR EMBL; CBJP010000112; CDE77269.1; -; Genomic_DNA.
DR Proteomes; UP000018289; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009318; C:exodeoxyribonuclease VII complex; IEA:InterPro.
DR GO; GO:0008855; F:exodeoxyribonuclease VII activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd04489; ExoVII_LU_OBF; 1.
DR HAMAP; MF_00378; Exonuc_7_L; 1.
DR InterPro; IPR003753; Exonuc_VII_L.
DR InterPro; IPR020579; Exonuc_VII_lsu_C.
DR InterPro; IPR025824; OB-fold_nuc-bd_dom.
DR PANTHER; PTHR30008; PTHR30008; 1.
DR Pfam; PF02601; Exonuc_VII_L; 1.
DR Pfam; PF13742; tRNA_anti_2; 1.
DR TIGRFAMs; TIGR00237; xseA; 1.
PE 3: Inferred from homology;
KW Complete proteome {ECO:0000313|Proteomes:UP000018289};
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00378,
KW ECO:0000256|SAAS:SAAS00723549};
KW Exonuclease {ECO:0000256|HAMAP-Rule:MF_00378,
KW ECO:0000256|RuleBase:RU004355, ECO:0000256|SAAS:SAAS00723511};
KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_00378,
KW ECO:0000256|RuleBase:RU004355, ECO:0000256|SAAS:SAAS00723558};
KW Nuclease {ECO:0000256|HAMAP-Rule:MF_00378,
KW ECO:0000256|RuleBase:RU004355, ECO:0000256|SAAS:SAAS00723518};
KW Reference proteome {ECO:0000313|Proteomes:UP000018289}.
FT DOMAIN 15 107 tRNA_anti_2. {ECO:0000259|Pfam:PF13742}.
FT DOMAIN 131 440 Exonuc_VII_L. {ECO:0000259|Pfam:PF02601}.
SQ SEQUENCE 444 AA; 49763 MW; 686E0A7ED4F5E7C2 CRC64;
MFFEQNTSLS SSSVWTVSQL NDEVSRSLQM NFPPLWVGGE VRGFTRAASG HWYFTLKDAT
GELSCAMFAG NNRRVGFIPK TGDHLEVHGQ VSIYRARGSY QMVVDAVRQA GLGTLYERFL
QLKEKLQKEG LFDLSRKKTI ARINTKIAVV TSLQAAALRD VLTTLRRRAP YARLIVFETA
VQGDEAPKEI ISALKRADQS DVDVILLVRG GGSLQDLWSF NDEAVARSVA ALSKPVIVGV
GHESDVTIVD WVADLRAATP TAAAEHATES VDVLLKELAQ THETMRYVWS RYWTENTQKV
DSLMREIPDP AKSLRQANER LTLLSQSLSS LMRSQWFVKS ETVRHLSASL INPASLFLRE
KERHDRISKE LVSLIHDKLN QNERARQSLM DLITEISPQA ILKKGYSYVT ASDGTFVKSA
ESAVKSDNLQ IHWHDGQLEV HVKK
//
