ID R7KZN1_9FIRM Unreviewed; 455 AA.
AC R7KZN1;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=Glycoside hydrolase family 4 {ECO:0000313|EMBL:CDE81183.1};
GN ORFNames=BN622_01980 {ECO:0000313|EMBL:CDE81183.1};
OS Ruminococcus sp. CAG:353.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=1262955 {ECO:0000313|EMBL:CDE81183.1, ECO:0000313|Proteomes:UP000017936};
RN [1] {ECO:0000313|EMBL:CDE81183.1, ECO:0000313|Proteomes:UP000017936}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:353 {ECO:0000313|Proteomes:UP000017936};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|RuleBase:RU361152};
CC Note=Binds 1 NAD(+) per subunit. {ECO:0000256|RuleBase:RU361152};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family.
CC {ECO:0000256|ARBA:ARBA00010141, ECO:0000256|RuleBase:RU361152}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDE81183.1}.
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DR EMBL; CBJQ010000131; CDE81183.1; -; Genomic_DNA.
DR AlphaFoldDB; R7KZN1; -.
DR Proteomes; UP000017936; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.90.1820.10; AglA-like glucosidase; 1.
DR InterPro; IPR001088; Glyco_hydro_4.
DR InterPro; IPR022616; Glyco_hydro_4_C.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR32092; 6-PHOSPHO-BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR32092:SF2; ALPHA-GALACTURONIDASE; 1.
DR Pfam; PF02056; Glyco_hydro_4; 1.
DR Pfam; PF11975; Glyco_hydro_4C; 1.
DR PRINTS; PR00732; GLHYDRLASE4.
DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361152};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361152};
KW NAD {ECO:0000256|RuleBase:RU361152};
KW Reference proteome {ECO:0000313|Proteomes:UP000017936}.
FT DOMAIN 206..426
FT /note="Glycosyl hydrolase family 4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11975"
FT SITE 113
FT /note="Increases basicity of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-4"
SQ SEQUENCE 455 AA; 50290 MW; DC717D5A0F2A4FB8 CRC64;
MNSERLNIAY IGGGSDNFGW KLIPELSGED ICAMVRLYDT DKSRALANEV IGNGIHDHGG
VKGDVVYLAC DEAEEALRDA DFVILSFDPG SLEELVTELH LPETYGIFQP SVENSGPGSV
IRALKIMPVC AEYARKISEL CPNAWVISLC TPMAECISVM KKVFPKMKLV GADCSTHSCR
DMLATIVCES KGITGVRRRD IKTNLLGIDG YSWFDEITFC GEDLFPMFTE YAEKYVSNGY
EFRANEYKNN PDADAHKVKF DLFLRYGLIP AQNDRITAGF CPPWYTGNVK EMNSWKFSPI
TAAYKKKIVS DKASRVKRYM NGEPLPKSAG TTEVAAMVRA LAGGGNFICP VIMPNHGQVE
NLPEGTAVQT NALISRGAVR PVAAGALPED ILCLTLRHCG NQAQVVRAYE TKDLDVAFNG
FLNDPEMTCG LNDATELYRD MLSEVRKHLI YYCED
//
