ID   R7R047_9FIRM            Unreviewed;       460 AA.
AC   R7R047;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   SubName: Full=2-isopropylmalate synthase {ECO:0000313|EMBL:CDF43061.1};
GN   ORFNames=BN520_02136 {ECO:0000313|EMBL:CDF43061.1};
OS   Roseburia sp. CAG:182.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia.
OX   NCBI_TaxID=1262942 {ECO:0000313|EMBL:CDF43061.1, ECO:0000313|Proteomes:UP000018104};
RN   [1] {ECO:0000313|EMBL:CDF43061.1, ECO:0000313|Proteomes:UP000018104}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:182 {ECO:0000313|Proteomes:UP000018104};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDF43061.1}.
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DR   EMBL; CBKT010000273; CDF43061.1; -; Genomic_DNA.
DR   AlphaFoldDB; R7R047; -.
DR   STRING; 1262942.BN520_02136; -.
DR   Proteomes; UP000018104; Unassembled WGS sequence.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   CDD; cd07947; DRE_TIM_Re_CS; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR000891; PYR_CT.
DR   PANTHER; PTHR42880; HOMOCITRATE SYNTHASE; 1.
DR   PANTHER; PTHR42880:SF1; ISOPROPYLMALATE_HOMOCITRATE_CITRAMALATE SYNTHASE FAMILY PROTEIN; 1.
DR   Pfam; PF00682; HMGL-like; 1.
DR   SUPFAM; SSF51569; Aldolase; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000018104};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          58..328
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000259|PROSITE:PS50991"
SQ   SEQUENCE   460 AA;  52988 MW;  6FD00932F2F185AD CRC64;
     MREVVMNDKT NLLQLEEHFY QLADVTEPNT FRNLFPYTEI PKIAFNDRIV PHNMPEEIWI
     TDTTFRDGQQ SRAPYTTEQI VTIYDYLHKL GGPKGKIRQS EFFLYSKKDR DAVYKCLERG
     YKFPEVTSWI RASKQDFQLV KDLGLRETGI LVSCSDYHIF YKMKMTRREV MNLYLSVIRE
     CLETGISPRC HLEDITRSDI YGFVIPFCLE LMKLMDEYKI PIKVRVCDTM GYGVNFPGAV
     IPRSIPGIIY GLTTHAGVPS ELIEFHGHND FYKAVNNSTT AWLYGASGVN CALFGIGERT
     GNTPLEAMVF EYAQLRGTLD GMDTTVITEL AEYYEKELDY HIPDRTPFVG KNFNVTRAGI
     HADGLLKNEE IYNIFDTEKF LNRPALVSVS NTSGLAGIAH WINSYYRLSK DRWVDKNSEL
     VKMIKEWVDK QYEEGRVTVL TDNELVVEIT DCCKKLGIVL
//