ID R7XUH2_9ACTN Unreviewed; 1195 AA.
AC R7XUH2;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 24-JAN-2024, entry version 47.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=CF8_3169 {ECO:0000313|EMBL:EON22931.1};
OS Nocardioides sp. CF8.
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Nocardioidaceae; Nocardioides.
OX NCBI_TaxID=110319 {ECO:0000313|EMBL:EON22931.1, ECO:0000313|Proteomes:UP000015971};
RN [1] {ECO:0000313|EMBL:EON22931.1, ECO:0000313|Proteomes:UP000015971}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CF8 {ECO:0000313|EMBL:EON22931.1,
RC ECO:0000313|Proteomes:UP000015971};
RX PubMed=23833136;
RA Kimbrel J.A., Chang J., Arp D.J., Sayavedra-Soto L.A.;
RT "The Draft Genome Sequence of Nocardioides sp. Strain CF8 Reveals the Scope
RT of Its Metabolic Capabilities.";
RL Genome Announc. 1:e00439-e00439(2013).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EON22931.1}.
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DR EMBL; ASEP01000097; EON22931.1; -; Genomic_DNA.
DR AlphaFoldDB; R7XUH2; -.
DR STRING; 110319.CF8_3169; -.
DR PATRIC; fig|110319.3.peg.3830; -.
DR eggNOG; COG1197; Bacteria.
DR HOGENOM; CLU_005122_2_2_11; -.
DR Proteomes; UP000015971; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000015971}.
FT DOMAIN 661..822
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 840..997
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1195 AA; 130942 MW; C31D964B8095737D CRC64;
MHADVDAVME VVVQDHKTPL TAVADAVLAG PVLAEAMAEA SQVRALDLTG PAAVRPLVVK
GLADAGRTVL AVTATAREAE DLVVELADLL DPATIAYYPS WETLPHERLS PRSDTVGRRL
AVLRRLKHPG TDASNGPLRV IVAPVRSILQ PQVKGLADLE PVELTKGSTA ALDDVVRGLA
DAAYSRVDMV EKRGEFAVRG GIVDVFPPTE EHPLRVEFWG DEVEEIRSFS VADQRTVETL
DRLWAPPCRE LLLTEDVRRR AAALGQAHPQ LLELTDKIAA GIAVEGMESL APVLVDEMEL
LVDLLPDTTT VLVLDPERAR TRAHDLVATS EEFLGASWAA AASGGQSPID LGAASYREVA
DVRGSVIERS LGWWTVSPFG IDSQELDVEV IAGAVPSRAL SVQGAEAYRG DLEKAIADIR
GWLAQGYRVT VVHQGHGPAE RMVEVLGEHD VPAALIDGAV SPDASVVTVT TGCLVSGFID
PETKVAVLTG EDISGQKGST RDMRKMPARR KKQIDPLELK AGDYVVHEQH GVGRFVAMQQ
REMQGATREY LVLEYGSSKR GAPADRLFVP ADTLDQITRY VGGEQPSLDR LGGGDWTKRK
NRARKAVREI AAELIKLYAA RQATKGHAFG PDTPWQRELE DAFPFHETPD QLTTVDEVKA
DMRRTVPMDR LVCGDVGYGK TEIAVRAAFK AVQDGKQVAV LVPTTLLVTQ HLSTFTERMS
GFPVVLKGLS RFQTDKEARE VVAGLEDGSI DIVVGTHRLL SPNIKMKDLG LIIVDEEQRF
GVEHKEQMKR LRTSVDVLSM SATPIPRTLE MAVTGIREMS TITTPPEERH PVLTYVGAYE
DRQVIAAVRR ELLREGQVFF IHNRVNSIEK AAAHIRELVP EARVATAHGQ MGEHQLEQVM
LDFWEKRFDV LVCTTIVESG LDVSNANTMI IERADTLGLS QLHQLRGRVG RSRERAYAYF
LYPAEKPLTE TAHERLATLA QHSDLGGGMA IAMKDLEIRG AGNLLGGEQS GHIADVGFDL
YVRLVGEAVN DFKGEGAEEL GEVRIELPVD AHLPHDYISS ERLRLEMYKR LAEVRADEDV
DQLREEMVDR YGQPPIEVES LLIVARFRAR ARQAGVKEIT LAGKFVRFAP VDLPDSRVVR
LGRVYPKSIV KAPTQTILVP RPQTAVIGGK PIDGIALLEW ARHVIDDIID PPTTS
//
