ID R9IFF6_9FIRM Unreviewed; 471 AA.
AC R9IFF6;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=C804_06300 {ECO:0000313|EMBL:EOS20546.1};
OS Lachnospiraceae bacterium A4.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=397291 {ECO:0000313|EMBL:EOS20546.1, ECO:0000313|Proteomes:UP000014118};
RN [1] {ECO:0000313|EMBL:EOS20546.1, ECO:0000313|Proteomes:UP000014118}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A4 {ECO:0000313|EMBL:EOS20546.1,
RC ECO:0000313|Proteomes:UP000014118};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Lachnospiraceae bacterium A4.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOS20546.1}.
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DR EMBL; ASSR01000162; EOS20546.1; -; Genomic_DNA.
DR AlphaFoldDB; R9IFF6; -.
DR STRING; 397291.C804_06300; -.
DR PATRIC; fig|397291.3.peg.6546; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_013049_0_1_9; -.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000014118; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000014118};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 13..164
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 178..447
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 471 AA; 52887 MW; EB03946AE6D4F2EC CRC64;
MIDNKKEQER DELHRTIWNM ANDLRGSVDG WDFKQYVLGM LFYRYISENF VKFVNAGEVE
AGNDNFDYAK LDNEIAEEAR EDLVQSKGYF ILPSQLFCNV KEKAPNNENL NETLESVFRN
IEASAQGHDS EDDFNGLFDD IDVNSNKLGG TVAKRNEKLV KLLNGVAEMK LGDYQDNTID
AFGDAYEFLM GMYASNAGKS GGEYYTPQEV SELLTRITVL GKKEVNKVYD PACGSGSLLL
KFAKVLGKEN VRQGFFGQEI NITTYNLCRI NMFLHDIGYE KFSIGHGDTL TEPLHWDDEP
FEAIVSNPPY SIKWEGDDNP ILINDERFSP AGVLAPKSKA DLAFIMHSLS WLATNGTAAI
VCFPGVMYRG GAEQKIRKYL IDNNYVECVI QLPDNLFFGT SIATCIMVLK KNKVENSTLF
IDASKECVKI TNNNKLTEEN IQNISTCAVA IWLGAAIKQQ KYNSHILPSA L
//
