ID R9JEX5_9FIRM Unreviewed; 805 AA.
AC R9JEX5;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=C807_00520 {ECO:0000313|EMBL:EOS32755.1};
OS Lachnospiraceae bacterium 28-4.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=397287 {ECO:0000313|EMBL:EOS32755.1, ECO:0000313|Proteomes:UP000014112};
RN [1] {ECO:0000313|EMBL:EOS32755.1, ECO:0000313|Proteomes:UP000014112}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=28-4 {ECO:0000313|EMBL:EOS32755.1,
RC ECO:0000313|Proteomes:UP000014112};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Lachnospiraceae bacterium 28-4.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOS32755.1}.
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DR EMBL; ASSU01000008; EOS32755.1; -; Genomic_DNA.
DR AlphaFoldDB; R9JEX5; -.
DR STRING; 397287.C807_00520; -.
DR PATRIC; fig|397287.3.peg.563; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_8_9; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000014112; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000014112}.
FT DOMAIN 5..285
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 286..560
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 704..731
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 805 AA; 90879 MW; D3A9034B42BCEDF2 CRC64;
MSIYDTLNEQ QKRGVFAVDG PVLLLAGAGS GKTRVLTHRI AYLIDEIGVS PYHIMAITFT
NKAAGEMRER VDSIIGHGAN QVWVSTFHAA CVRILRRYID RIGFDNNFTI YDMDDQKTVM
KDVCKRLQID TKMLKERAIL SAISSAKDEL ISPQDYQIQA MGDFHGERIA RAYVEYQETL
RKSNALDFDD LIVKTVELFK TCPEVLAGYQ DRFRYLMVDE YQDTNTVQFE FIRLLADKYR
NLCVVGDDDQ SIYKFRGANI RNILDFEEVY PEATVIKLEQ NYRSTQNVLD AANAVIKNNR
GRKEKALWTD KGEGNKVHFR QFDTAYEEAE FIAGDVGSKK RRGIADYGDF AVLYRTNAQA
RMLEERFILE GIPYDVVGGT NFYARREIKD ILAYLKTVDN GRDDVAVKRI INIPKRGIGA
TTITRVQEYA DERNISFYDA LREADQIMTI GRSAGKLAPF VTMIQGFRSK LEYYGLEGLI
KDIIETTGYV KELEASGEED AEDRIHNIDE LISKVVAYEE THEEAGLSGF LEEVALVADI
DRIEGESSRV LLMTLHSAKG LEFPYVYLAG MEDGVFPGYM AITSDDREDI EEERRLAYVG
ITRAKEDLTL TCAKQRMVRG ETQYNAVSRF VEEIPAELLD QKVPSLKKWN YVDYDEGSEE
KLRFRAKPFG MDQTERGDAG GDFRGNASVN GGADSYQRIF HKKQTDSARH KPRATAVPKR
TPAENRPFIA GGGAGSLKSV NGLSKGAPEA EAVNYGVGDR VRHIKYGEGT VMKLEKGPRD
YQVTVVFDGA GQKIMYAAFA KLKKL
//
