ID R9KNF1_9FIRM Unreviewed; 1013 AA.
AC R9KNF1;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00013303, ECO:0000256|RuleBase:RU361154};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230, ECO:0000256|RuleBase:RU361154};
GN ORFNames=C809_03165 {ECO:0000313|EMBL:EOS45032.1};
OS Lachnospiraceae bacterium MD335.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=1235793 {ECO:0000313|EMBL:EOS45032.1, ECO:0000313|Proteomes:UP000014081};
RN [1] {ECO:0000313|EMBL:EOS45032.1, ECO:0000313|Proteomes:UP000014081}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=COE1 {ECO:0000313|EMBL:EOS45032.1,
RC ECO:0000313|Proteomes:UP000014081};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Lachnospiraceae bacterium COE1.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU361154};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401, ECO:0000256|RuleBase:RU361154}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOS45032.1}.
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DR EMBL; ASSW01000050; EOS45032.1; -; Genomic_DNA.
DR AlphaFoldDB; R9KNF1; -.
DR STRING; 1235793.C809_03165; -.
DR PATRIC; fig|1235793.3.peg.3283; -.
DR eggNOG; COG3250; Bacteria.
DR HOGENOM; CLU_002346_0_2_9; -.
DR OrthoDB; 9762066at2; -.
DR Proteomes; UP000014081; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR023232; Glyco_hydro_2_AS.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR023230; Glyco_hydro_2_CS.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF16353; LacZ_4; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361154};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361154};
KW Reference proteome {ECO:0000313|Proteomes:UP000014081}.
FT DOMAIN 740..1008
FT /note="Beta galactosidase small chain/"
FT /evidence="ECO:0000259|SMART:SM01038"
SQ SEQUENCE 1013 AA; 116595 MW; 68D779B0012CB7EA CRC64;
MIVPRYYENL EVLHEGTMPA RAYYMPASKR MDYLVEHREH SDRVQFLNGL WKFCYYESIY
AVKDRFYEPG YDTSDFDTLN VPGVWQMAGY DAHQYTNIKY PFPFDPPFVP QDIPCGAYVY
DFEYQTDQQA PRAYLNFEGV DSCFYVWLNG TCIGYSQVSH ATSEFDVTQA LVEGTNTLAV
LVLKWCDGSY LEDQDKLRMS SIFRDVYLLR RPKHAVRDYR IETLTKDKRG RIQCSFSYME
QPVDTTVSVY DGKNNMVASG KVQADRISLE VIQPDLWSAE SPNLYTVVIE TQDETITDYT
GFRTIEIRDK VIYLNGQKIK FHGVNRHDCD PVTGAAVSMP QVIKDLTMMK RHNFNAIRSS
HYPNAPYFYQ LCDRYGFMVI DEADIEAHGP FMLYYKEDTD YNRFKGWNAQ IADNPAWERS
ILDRVRSMVQ RDKNRPCILI WSMGNESAYG CNFEKALRWT KEFDPGRLTQ YESARYRNYD
VTYDYSDLDL YSRMYPSFPE MEAYLKKDAG KPFLLVEYCH AMGNGPGDLE DYFWVIQSDD
KMCGGFVWEW CDHAVYAGTA DNGKAKYLYG GDFGETLHDG NFCVDGLVYP DRTPHTGLLE
YKNVHRPARV VYYHEPSKEL KLHNYLDFTD LKDYAVIDYE VTCDGVCVDA GSIPSFSVLP
HQEAVTTLDI TVPNSGRAYL RIIYRHLGAS PLIPEGYVLG FDEILLVNAD PRNQTAVKWL
YPVIGTDAAI DVTETDDAVL LRGKDFTYTY NRKNGLFARV NYAGREYLDQ PMEINLWRAP
TDNDMYIKQE WEKARYKEAY TRAYETVVKQ SKDGVAIMGT LSVCAASVQR IMDIKAVWNV
DYNGGIRMTM DVVRNTELPV LPRFGIRLFL SGQLEDVSYY GYGPTESYRD KHQATAHGLY
RANVAHMHED YLRPQENGSH FDCDYVALCA GAYGIAAVSE QRFSFNASVY TQEELEKKKH
HFVLEKSGSS VLCLDYALNG IGSNSCGPEV LERYCFDDAQ FCYGLKLVPF VKG
//
