ID RFCS_PYRHO Reviewed; 855 AA.
AC O57852;
DT 16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 27-MAR-2024, entry version 133.
DE RecName: Full=Replication factor C small subunit;
DE Short=RFC small subunit;
DE AltName: Full=Clamp loader small subunit;
DE Contains:
DE RecName: Full=Pho RFC intein;
GN Name=rfcS; OrderedLocusNames=PH0112;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- FUNCTION: Part of the RFC clamp loader complex which loads the PCNA
CC sliding clamp onto DNA. {ECO:0000250}.
CC -!- SUBUNIT: Heteromultimer composed of small subunits (RfcS) and large
CC subunits (RfcL). {ECO:0000250}.
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- MISCELLANEOUS: The intein interrupts the potential ATP-binding site.
CC -!- SIMILARITY: Belongs to the activator 1 small subunits family. RfcS
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BA000001; BAA29181.1; -; Genomic_DNA.
DR PIR; F71231; F71231.
DR AlphaFoldDB; O57852; -.
DR SMR; O57852; -.
DR STRING; 70601.gene:9377020; -.
DR EnsemblBacteria; BAA29181; BAA29181; BAA29181.
DR KEGG; pho:PH0112; -.
DR eggNOG; arCOG00469; Archaea.
DR eggNOG; arCOG03154; Archaea.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00009; AAA; 1.
DR CDD; cd00081; Hint; 1.
DR CDD; cd18140; HLD_clamp_RFC; 1.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 1.20.272.10; -; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR013748; Rep_factorC_C.
DR InterPro; IPR047854; RFC_lid.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR PANTHER; PTHR11669; REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT; 1.
DR PANTHER; PTHR11669:SF5; REPLICATION FACTOR C SUBUNIT 2; 1.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 2.
DR Pfam; PF08542; Rep_fac_C; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR SUPFAM; SSF48019; post-AAA+ oligomerization domain-like; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Autocatalytic cleavage; DNA replication; Nucleotide-binding;
KW Protein splicing.
FT CHAIN 1..61
FT /note="Replication factor C small subunit, 1st part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030379"
FT CHAIN 62..586
FT /note="Pho RFC intein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030380"
FT CHAIN 587..855
FT /note="Replication factor C small subunit, 2nd part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030381"
FT DOMAIN 185..308
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
SQ SEQUENCE 855 AA; 98422 MW; FAD533FFD08FCF27 CRC64;
MHNMEEVREV KVLEKPWVEK YRPQRLDEIV GQEHIVKRLK HYVKTGSMPH LLFAGPPGVG
KCLTGDTKVI ANGQLFELRE LVEKISGGKF GPTPVKGLKV IGIDEDGKLR EFEVQYVYKD
KTERLIRIRT RLGRELKVTP YHPLLVNRRN GEIKWVKAEE LKPGDKLAVP RFLPIVTGED
PLAEWLGYFL GGGYADSKEN LIMFTNEDPL LRQRFMELTE KLFSDARIRE ITHENGTSKV
YVNSKKALKL VNSLGNAHIP KECWRGIRSF LRAYFDCNGG VKGNAIVLAT ASKEMSQEIA
YALAGFGIIS RIQEYRVIIS GSDNVKKFLN EIGFINRNKL EKALKLVKKD DPGHDGLEIN
YELISYVKDR LRLSFFNDKR SWSYREAKEI SWELMKEIYY RLDELEKLKE SLSRGILIDW
NEVAKRIEEV AEETGIRADE LLEYIEGKRK LSFKDYIKIA KVLGIDVEHT IEAMRVFARK
YSSYAEIGRR LGTWNSSVKT ILESNAVNVE ILERIRKIEL ELIEEILSDE KLKEGIAYLI
FLSQNELYWD EITKVEELRG EFIIYDLHVP GYHNFIAGNM PTVVHNTTAA LALSRELFGE
NWRHNFLELN ASDERGINVI REKVKEFART KPIGGASFKI IFLDEADALT QDAQQALRRT
MEMFSSNVRF ILSCNYSSKI IEPIQSRCAI FRFRPLRDED IAKRLRYIAE NEGLELTEEG
LQAILYIAEG DMRRAINILQ AAAALDKKIT DENVFMVASR ARPEDIREMM LLALKGNFLK
AREKLREILL KQGLSGEDVL IQMHKEVFNL PIDEPTKVYL ADKIGEYNFR LVEGANEMIQ
LEALLAQFTL VGKKK
//
