ID RHAS_ECOHS Reviewed; 278 AA.
AC A8A709;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 1.
DT 27-MAR-2024, entry version 103.
DE RecName: Full=HTH-type transcriptional activator RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
DE AltName: Full=L-rhamnose operon regulatory protein RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
GN Name=rhaS {ECO:0000255|HAMAP-Rule:MF_01534}; OrderedLocusNames=EcHS_A4135;
OS Escherichia coli O9:H4 (strain HS).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=331112;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HS;
RX PubMed=18676672; DOI=10.1128/jb.00619-08;
RA Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA Henderson I.R., Sperandio V., Ravel J.;
RT "The pangenome structure of Escherichia coli: comparative genomic analysis
RT of E. coli commensal and pathogenic isolates.";
RL J. Bacteriol. 190:6881-6893(2008).
CC -!- FUNCTION: Activates expression of the rhaBAD and rhaT operons.
CC {ECO:0000255|HAMAP-Rule:MF_01534}.
CC -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01534}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01534}.
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DR EMBL; CP000802; ABV08313.1; -; Genomic_DNA.
DR RefSeq; WP_000217137.1; NC_009800.1.
DR AlphaFoldDB; A8A709; -.
DR SMR; A8A709; -.
DR GeneID; 75204579; -.
DR KEGG; ecx:EcHS_A4135; -.
DR HOGENOM; CLU_000445_88_5_6; -.
DR Proteomes; UP000001123; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd06977; cupin_RhaR_RhaS-like_N; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR HAMAP; MF_01534; HTH_type_RhaS; 1.
DR InterPro; IPR003313; AraC-bd.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR037923; HTH-like.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR047220; RhaR_RhaS-like_N.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR InterPro; IPR023609; Tscrpt_reg_HTH_RhaS.
DR PANTHER; PTHR46796:SF2; HTH-TYPE TRANSCRIPTIONAL ACTIVATOR RHAS; 1.
DR PANTHER; PTHR46796; HTH-TYPE TRANSCRIPTIONAL ACTIVATOR RHAS-RELATED; 1.
DR Pfam; PF02311; AraC_binding; 1.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 2.
DR SUPFAM; SSF51215; Regulatory protein AraC; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Reference proteome; Repeat;
KW Rhamnose metabolism; Transcription; Transcription regulation.
FT CHAIN 1..278
FT /note="HTH-type transcriptional activator RhaS"
FT /id="PRO_1000068701"
FT DOMAIN 174..272
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT DNA_BIND 191..212
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT DNA_BIND 239..262
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT SITE 241
FT /note="Interaction with sigma-70"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT SITE 250
FT /note="Interaction with sigma-70"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
SQ SEQUENCE 278 AA; 32315 MW; 64264514484C0D2A CRC64;
MTVLHSVDFF PSGNASVAIE PRLPQADFPE HHHDFHEIVI VEHGTGIHVF NGQPYTITGG
TVCFVRDHDR HLYEHTDNLC LTNVLYRSPD RFQFLAGLNQ LLPQELDGQY PSHWRVNHSV
LQQVRQLVAQ MEQQEGENDL PSTASREILF MQLLLLLRKS SLQENLENSA SRLNLLLAWL
EDHFADEVNW DAVADQFSLS LRTLHRQLKQ QTGLTPQRYL NRLRLMKARH LLRHSEASVT
DIAYRCGFSD SNHFSTLFRR EFNWSPRDIR QGRDGFLQ
//
