ID RNC_CAEBR Reviewed; 1061 AA.
AC Q61XX9; A8WWR6;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 24-JAN-2024, entry version 103.
DE RecName: Full=Ribonuclease 3;
DE EC=3.1.26.3;
DE AltName: Full=Protein drosha;
DE AltName: Full=Ribonuclease III;
DE Short=RNase III;
GN Name=drsh-1 {ECO:0000250|UniProtKB:O01326}; ORFNames=CBG03798;
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Executes the initial step of microRNA (miRNA) processing in
CC the nucleus, that is the cleavage of pri-miRNA to release pre-miRNA.
CC Involved in pre-rRNA processing. Cleaves double-strand RNA and does not
CC cleave single-strand RNA. Involved in fertility. Required for the
CC function or synthesis of the let-7 miRNA (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.3;
CC Evidence={ECO:0000255};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9NRR4}.
CC -!- SIMILARITY: Belongs to the ribonuclease III family. {ECO:0000305}.
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DR EMBL; HE600906; CAP24627.1; -; Genomic_DNA.
DR RefSeq; XP_002639240.1; XM_002639194.1.
DR AlphaFoldDB; Q61XX9; -.
DR SMR; Q61XX9; -.
DR STRING; 6238.Q61XX9; -.
DR GeneID; 8581234; -.
DR KEGG; cbr:CBG_03798; -.
DR CTD; 8581234; -.
DR WormBase; CBG03798; CBP41277; WBGene00026581; Cbr-drsh-1.
DR eggNOG; KOG1817; Eukaryota.
DR HOGENOM; CLU_004383_1_0_1; -.
DR InParanoid; Q61XX9; -.
DR OMA; NDGPECR; -.
DR OrthoDB; 5486676at2759; -.
DR Proteomes; UP000008549; Chromosome I.
DR GO; GO:0070877; C:microprocessor complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004525; F:ribonuclease III activity; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007506; P:gonadal mesoderm development; IEA:UniProtKB-KW.
DR GO; GO:0031054; P:pre-miRNA processing; IBA:GO_Central.
DR GO; GO:0031053; P:primary miRNA processing; ISS:UniProtKB.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0019953; P:sexual reproduction; ISS:UniProtKB.
DR CDD; cd19877; DSRM_RNAse_III_meta_like; 1.
DR CDD; cd00593; RIBOc; 2.
DR Gene3D; 3.30.160.20; -; 1.
DR Gene3D; 1.10.1520.10; Ribonuclease III domain; 2.
DR HAMAP; MF_00104; RNase_III; 1.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR011907; RNase_III.
DR InterPro; IPR000999; RNase_III_dom.
DR InterPro; IPR044442; RNAse_III_DSRM__animal.
DR InterPro; IPR036389; RNase_III_sf.
DR PANTHER; PTHR11207:SF0; RIBONUCLEASE 3; 1.
DR PANTHER; PTHR11207; RIBONUCLEASE III; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF14622; Ribonucleas_3_3; 1.
DR Pfam; PF00636; Ribonuclease_3; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00535; RIBOc; 2.
DR SUPFAM; SSF54768; dsRNA-binding domain-like; 1.
DR SUPFAM; SSF69065; RNase III domain-like; 2.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS00517; RNASE_3_1; 2.
DR PROSITE; PS50142; RNASE_3_2; 2.
PE 3: Inferred from homology;
KW Developmental protein; Differentiation; Endonuclease;
KW Gonadal differentiation; Hydrolase; Magnesium; Manganese; Metal-binding;
KW Nuclease; Nucleus; Reference proteome; Repeat; Ribosome biogenesis;
KW RNA-binding; rRNA processing.
FT CHAIN 1..1061
FT /note="Ribonuclease 3"
FT /id="PRO_0000309457"
FT DOMAIN 586..759
FT /note="RNase III 1"
FT /evidence="ECO:0000255"
FT DOMAIN 811..935
FT /note="RNase III 2"
FT /evidence="ECO:0000255"
FT DOMAIN 962..1037
FT /note="DRBM"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 149..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 152..175
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..235
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 851
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 921
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 924
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT SITE 917
FT /note="Important for activity"
FT /evidence="ECO:0000250"
SQ SEQUENCE 1061 AA; 122481 MW; 796401EAD0B5138E CRC64;
MDFTEIHKRS RRKKFQQIHQ DRKDEMIQQL GRRFHNQPST SATYPSAVED IPLPSEVPNV
FGAPPPLTNA DFHRNFLVDP DVVVSHSASL IRSNRHIVKA EDAEQYMMVS RERVGTTAER
VLEDFNSRVI KPLKAKRRLQ IDVPYIDHPL HSMRSKTPER KENEEDSDSE IRSSDSSSDA
EYGSDVEEEP DSCRRKKRTH KIQKADSSQT KVEEKERQNT LLRMGIERKR NHPNAIDPHI
SYNEKGLGND SPECRCPFPI RNRGLKHGYY AGENQVLKCS KNDRANLHYY TLHVTPAPNE
SQIQKTQMLI NGMEYHFEGF TMVTHAPLPD CMTRRPVFKY SIDYEFQLIE EFMPTECFDP
EDCNSIFEYI FHDIFELLDF DLYPKHLPPG TASCPTIHIV PRFVAMENNT TFIWSSKTVL
AFFLLHGKNN MFSPEDVEKN CAMSDDAFGR TIAKLKQSIV LNPMKKPSAL RADWFSRDLE
NKEMFLIQNT IRSQNFASPF LPQIAALEKK MSRLKQEKKD SGNKNPHYEN LKAELIVLKD
KHREARQLKL KLPVKDYIDT GLKPDVVAHV AMAIIASHHI RYNFSLSVFE KVIEYKFNDR
RIVELALIHS SFRSYYGTTP DHVKNMISNC GYRKKYGAEE RREKKKGIIS LFNIMGGETS
GGEPILHNER LEYLGDAVVE LIASHHLFFI LNHHFEGGLA TYRTALVQNR NLAKLAMNCR
IDEMLQFAHG ADLINEAEWK HALANAFEAL MAGVFLDSGI APCDAIFSKA MYGKDPEMKK
VWDHLNEHEL KIEDPLGDRD LSRITPALTD FHRLEQIIGI EFNNIRLLAK AFTRRNVPFN
DLTKGHNQRL EWLGDSVLQL IISDYLYRNF PLHHEGHMSL LRTSLVSNQT QSVVCDDLGF
QEFVIKAPHR KNDLKMKDKA DLVEAFIGAL YVDKGLEYCR SFIRTVFCPR LKHFINSEKW
NDAKSHLQQW CLAIRDSRNP NPAMPEYRLL GIQGPTNNRI FRVAVYFRGE RLSSAAASNM
HTAELKAAEN ALAALEKASF SRMREKYMSG RQHRLHRIFF S
//
