ID RPOA_CLOD6 Reviewed; 315 AA.
AC Q18CI5;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 1.
DT 27-MAR-2024, entry version 109.
DE RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000255|HAMAP-Rule:MF_00059};
DE Short=RNAP subunit alpha {ECO:0000255|HAMAP-Rule:MF_00059};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_00059};
DE AltName: Full=RNA polymerase subunit alpha {ECO:0000255|HAMAP-Rule:MF_00059};
DE AltName: Full=Transcriptase subunit alpha {ECO:0000255|HAMAP-Rule:MF_00059};
GN Name=rpoA {ECO:0000255|HAMAP-Rule:MF_00059}; OrderedLocusNames=CD630_00980;
OS Clostridioides difficile (strain 630) (Peptoclostridium difficile).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC Clostridioides.
OX NCBI_TaxID=272563;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=630;
RX PubMed=16804543; DOI=10.1038/ng1830;
RA Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N.,
RA Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H.,
RA Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N.,
RA Brooks K., Chillingworth T., Cronin A., Davis P., Dowd L., Fraser A.,
RA Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K.,
RA Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L.,
RA Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J.;
RT "The multidrug-resistant human pathogen Clostridium difficile has a highly
RT mobile, mosaic genome.";
RL Nat. Genet. 38:779-786(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_00059}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_00059};
CC -!- SUBUNIT: Homodimer. The RNAP catalytic core consists of 2 alpha, 1
CC beta, 1 beta' and 1 omega subunit. When a sigma factor is associated
CC with the core the holoenzyme is formed, which can initiate
CC transcription. {ECO:0000255|HAMAP-Rule:MF_00059}.
CC -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and basal
CC transcription, whereas the C-terminal domain is involved in interaction
CC with transcriptional regulators and with upstream promoter elements.
CC {ECO:0000255|HAMAP-Rule:MF_00059}.
CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC {ECO:0000255|HAMAP-Rule:MF_00059}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AM180355; CAJ66917.1; -; Genomic_DNA.
DR RefSeq; WP_003427733.1; NZ_JAUPES010000043.1.
DR RefSeq; YP_001086566.1; NC_009089.1.
DR PDB; 7L7B; EM; 3.26 A; A/B=1-315.
DR PDBsum; 7L7B; -.
DR AlphaFoldDB; Q18CI5; -.
DR EMDB; EMD-23210; -.
DR SMR; Q18CI5; -.
DR STRING; 272563.CD630_00980; -.
DR ChEMBL; CHEMBL2363852; -.
DR DrugCentral; Q18CI5; -.
DR EnsemblBacteria; CAJ66917; CAJ66917; CD630_00980.
DR GeneID; 66352600; -.
DR KEGG; cdf:CD630_00980; -.
DR KEGG; pdc:CDIF630_00168; -.
DR PATRIC; fig|272563.120.peg.108; -.
DR eggNOG; COG0202; Bacteria.
DR OrthoDB; 9805706at2; -.
DR PhylomeDB; Q18CI5; -.
DR BioCyc; PDIF272563:G12WB-156-MONOMER; -.
DR PRO; PR:Q18CI5; -.
DR Proteomes; UP000001978; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule.
DR CDD; cd06928; RNAP_alpha_NTD; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR011773; DNA-dir_RpoA.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR011260; RNAP_asu_C.
DR InterPro; IPR036643; RNApol_insert_sf.
DR NCBIfam; TIGR02027; rpoA; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF03118; RNA_pol_A_CTD; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF47789; C-terminal domain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase.
FT CHAIN 1..315
FT /note="DNA-directed RNA polymerase subunit alpha"
FT /id="PRO_0000264492"
FT REGION 1..228
FT /note="Alpha N-terminal domain (alpha-NTD)"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00059"
FT REGION 245..315
FT /note="Alpha C-terminal domain (alpha-CTD)"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00059"
FT STRAND 8..11
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 22..29
FT /evidence="ECO:0007829|PDB:7L7B"
FT HELIX 32..46
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 49..58
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 63..66
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:7L7B"
FT HELIX 76..82
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 90..93
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 95..104
FT /evidence="ECO:0007829|PDB:7L7B"
FT HELIX 109..111
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 135..147
FT /evidence="ECO:0007829|PDB:7L7B"
FT HELIX 151..154
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 173..176
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 186..188
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 191..200
FT /evidence="ECO:0007829|PDB:7L7B"
FT STRAND 202..204
FT /evidence="ECO:0007829|PDB:7L7B"
FT HELIX 206..220
FT /evidence="ECO:0007829|PDB:7L7B"
SQ SEQUENCE 315 AA; 34920 MW; E75526C3C02D9B16 CRC64;
MIEIEKPKVD IVELSEDYRY GKFVIEPLER GYGITIGNAL RRILLSSLPG VAVNAIKIDG
VLHEFSTIPG VKEDVTEIIL TLKELSATID GEGSRTLKIE AQGPCSITGA DIICPPDVEI
LSKDLAIATL DDNAKLNMEI FVDKGRGYVS AEENKTENVP IGVLPVDSIY TPVEKVSYHV
ENTRVGQKTD YDKLVLEVWT NGSINPQEGI SLAAKVLVEH LNLFIDLTEH VSSVEIMVEK
EEDQKEKVLE MTIEELDLSV RSYNCLKRAG INTVEELANK SEDDMMKVRN LGKKSLEEVI
QKLEELGLGL KPSEE
//
