ID S0J3L4_9FIRM Unreviewed; 835 AA.
AC S0J3L4;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=C805_00929 {ECO:0000313|EMBL:EOT26827.1};
OS Eubacterium sp. 14-2.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=1235790 {ECO:0000313|EMBL:EOT26827.1, ECO:0000313|Proteomes:UP000014176};
RN [1] {ECO:0000313|EMBL:EOT26827.1, ECO:0000313|Proteomes:UP000014176}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=14-2 {ECO:0000313|EMBL:EOT26827.1,
RC ECO:0000313|Proteomes:UP000014176};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Xavier R., Elson C., Duck W., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Eubacterium bacterium 14-2.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOT26827.1}.
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DR EMBL; ASSS01000005; EOT26827.1; -; Genomic_DNA.
DR RefSeq; WP_016214000.1; NZ_KE159567.1.
DR AlphaFoldDB; S0J3L4; -.
DR STRING; 1235790.C805_00929; -.
DR PATRIC; fig|1235790.3.peg.980; -.
DR eggNOG; COG3250; Bacteria.
DR HOGENOM; CLU_005015_3_2_9; -.
DR OrthoDB; 9762066at2; -.
DR Proteomes; UP000014176; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000014176};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 187..307
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 678..763
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 777..833
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 835 AA; 96797 MW; C37C6448F76187D4 CRC64;
MIQQKLNNNW QMHGPGEPLR PAAVPGTVYT DLLRRGEMED PFWKDNEIRA LSLMEKDYEY
VTCFDAEKEL LASSRILLRF DGLDTIADIY LNGTQVGAAY NMHRIWEYDV THILKETGNE
LKVLLHSPLK FIRDAFAECR TLGSEDAMDG FVHIRKAHCM FGWDWGAHLP DAGIFREVTL
LGITGARLDS VYIRQEHTDG EVSLQFQVEA EREQAVSAHG CAVEKISVKN SGGDENPGSP
GVAYTVELTT PEGETRTYFD SPAEIHITSP SLWWPNGYGE QPLYQVKVTL YAEGTAVDVW
ERKIGLRTLT VKTEKDEWGV GFAHEINDIT IFAMGADYIP EDHLLGRVTP DTTRKLLEQC
VAANYNAVRV WGGGYYPEDW FYDICDELGL IVWQDFMFAC AVYELTPEFK ENIRQEFVDN
VKRLRHHPSL GLWCGNNEME MFVEEGHHWV TKKTEVRDYI LMYEQLIPEV LKIHDPQTFY
WPASPSSGGA FDEPNSPDRG DVHYWSVWHG NRPFSEYRKY FFRYASEFGF QSLPARKTLE
TITDDPRDMN LFSYVMEKHQ RQYGANGKIM NYLQQTYLYP SDFNTLIYAS QLLQADAIRY
GVEHFRRNRG RCMGAIVWQL NDCWPVISWS SIDYCGRWKA LHYMEKRFFA PLMISCQEEG
MMTKEADMNR EHFVFEKSIR LNVANETRAD QQVEVFWEVR NAAAEVLRRG EIRQVHVPAL
TSVWLEKVML PETDIFSEYV SFGMRQNGKV ISEGTVIFSY PKYFRYENPR LSFRLEEDEI
IISADAYAKS VEIRNEQEDF ILSDNYFDMN AGEKRVKILS GEPQGIRLRS VYDIR
//
