ID S1NTW0_9ENTE Unreviewed; 799 AA.
AC S1NTW0;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00012555};
DE EC=2.4.1.129 {ECO:0000256|ARBA:ARBA00012555};
GN ORFNames=I568_00798 {ECO:0000313|EMBL:EOW84304.1};
OS Enterococcus columbae DSM 7374 = ATCC 51263.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=1121865 {ECO:0000313|EMBL:EOW84304.1, ECO:0000313|Proteomes:UP000014113};
RN [1] {ECO:0000313|EMBL:EOW84304.1, ECO:0000313|Proteomes:UP000014113}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51263 {ECO:0000313|EMBL:EOW84304.1,
RC ECO:0000313|Proteomes:UP000014113};
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Russ C., Gilmore M., Surin D., Walker B., Young S., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Enterococcus columbae ATCC_51263 (PacBio/Illumina
RT hybrid assembly).";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.1.129;
CC Evidence={ECO:0000256|ARBA:ARBA00023988};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOW84304.1}.
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DR EMBL; ASWJ01000004; EOW84304.1; -; Genomic_DNA.
DR AlphaFoldDB; S1NTW0; -.
DR STRING; 1121865.OMW_00200; -.
DR PATRIC; fig|1121865.3.peg.192; -.
DR eggNOG; COG0744; Bacteria.
DR OrthoDB; 9766909at2; -.
DR Proteomes; UP000014113; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009002; F:serine-type D-Ala-D-Ala carboxypeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.12800; -; 1.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF32; PENICILLIN-BINDING PROTEIN 2A; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 4: Predicted;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000014113};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 36..62
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 93..271
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 403..645
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
FT REGION 775..799
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 799 AA; 88607 MW; AB25E1351FFB0494 CRC64;
MSDKNTRNHA KIKPEPKKTS IDKFLFFNIS VRVIQALFLV MMAFIILLTA LGLGIGMGYF
AFLVEDTKVP TKVELQQDLG DITQTSKLLY ADGTPIADIR SDLRRTNVPS NQISPWLKKA
IVATEDEYFY KHHGVVPKAV LRALLSEAIG GAGGGGSTLT QQLVKQQILS SETTFKRKAN
EILLALEVEK NFSKDEIITM YLNVSPFGRN NQGQNIAGVQ EAALGIFGKN ANDLTLPQAA
FIAGLPQSPI IYSPYTNDGQ LKKDFTYGLK RKDIVLFSMY RNHDITKKEY EAAKNYDLTK
DFLPPQQAEN TAHGYLYDEV MTQTIDLFIN KLATDDGLTN SQLDDPDIYN EYAQTAQEKL
ANGGYTVQTT IDANVYQAMQ EAVSDYGYML DIQTGDPVEV GNVLMENQTG KILGFVGGRD
YQTNQFNHAF DAKRQAGSAI KPVLVYGPAI DLGLIGSESR VSDYATTWKN GTDAGKEIVN
ATNKGSNTFQ TVREALSWSN NIPAYNIYQD IIQQTGDSSF VYTNYLAKMN YPASNNWQYE
SAPLGVTTVT ALTQTNGFQT LANRGVYQQG YMIESIKSAK GETIYQHTAN PVRVYSEAAA
TIMNDLMRSV LKDQITTSYK SNLASLNAQL ANFDWVGKTG STNDWADSWL IVSTPSITIS
SWSGYEDNRA TNSNAGKRTS LYLANLVNRI YQVKPTVFGN GETFSLADSV QKEKVAKFTG
QKNQGTVKLN NAEITNFGEE TTSLWAKNGP VDTTFKFGIG GTDANYTDYW KKFSSTKKTE
DKEEKDKKTE NQEQKNQEN
//
