ID S1PCB4_ECOLX Unreviewed; 1107 AA.
AC S1PCB4;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE SubName: Full=Potassium efflux system protein KefA {ECO:0000313|EMBL:EOW90844.1};
GN ORFNames=A13A_04808 {ECO:0000313|EMBL:EOW90844.1};
OS Escherichia coli KTE182.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=1181728 {ECO:0000313|EMBL:EOW90844.1, ECO:0000313|Proteomes:UP000014179};
RN [1] {ECO:0000313|EMBL:EOW90844.1, ECO:0000313|Proteomes:UP000014179}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KTE182 {ECO:0000313|EMBL:EOW90844.1,
RC ECO:0000313|Proteomes:UP000014179};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Feldgarden M., Nielsen K.L., Frimodt-Moller N., Andersen P.S., Walker B.,
RA Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A.,
RA Alvarado L., Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J.,
RA Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A.,
RA Larimer J., McCowan C., Murphy C., Neiman D., Pearson M., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Escherichia coli KTE182.";
RL Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family.
CC {ECO:0000256|ARBA:ARBA00008017}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOW90844.1}.
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DR EMBL; ASUG01000058; EOW90844.1; -; Genomic_DNA.
DR RefSeq; WP_001523757.1; NZ_KE136849.1.
DR AlphaFoldDB; S1PCB4; -.
DR PATRIC; fig|1181728.3.peg.4966; -.
DR HOGENOM; CLU_007829_2_0_6; -.
DR Proteomes; UP000014179; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR Gene3D; 1.10.287.1260; -; 1.
DR Gene3D; 2.30.30.60; -; 1.
DR Gene3D; 3.30.70.100; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR049142; MS_channel_1st.
DR InterPro; IPR049278; MS_channel_C.
DR InterPro; IPR023408; MscS_beta-dom_sf.
DR InterPro; IPR006685; MscS_channel_2nd.
DR InterPro; IPR011066; MscS_channel_C_sf.
DR InterPro; IPR006686; MscS_channel_CS.
DR InterPro; IPR011014; MscS_channel_TM-2.
DR InterPro; IPR025692; MscS_IM_dom1.
DR InterPro; IPR024393; MscS_porin.
DR PANTHER; PTHR30347:SF1; MINICONDUCTANCE MECHANOSENSITIVE CHANNEL MSCM; 1.
DR PANTHER; PTHR30347; POTASSIUM CHANNEL RELATED; 1.
DR Pfam; PF21088; MS_channel_1st; 1.
DR Pfam; PF00924; MS_channel_2nd; 1.
DR Pfam; PF21082; MS_channel_3rd; 1.
DR Pfam; PF12795; MscS_porin; 1.
DR Pfam; PF12794; MscS_TM; 1.
DR SUPFAM; SSF82689; Mechanosensitive channel protein MscS (YggB), C-terminal domain; 1.
DR SUPFAM; SSF82861; Mechanosensitive channel protein MscS (YggB), transmembrane region; 1.
DR SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
DR PROSITE; PS01246; UPF0003; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..1107
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004507339"
FT TRANSMEM 476..496
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 517..539
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 551..576
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 597..617
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 629..649
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 670..691
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 697..718
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 785..808
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 828..848
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 879..897
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 903..930
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 31..255
FT /note="Mechanosensitive ion channel MscS porin"
FT /evidence="ECO:0000259|Pfam:PF12795"
FT DOMAIN 478..814
FT /note="Mechanosensitive ion channel inner membrane"
FT /evidence="ECO:0000259|Pfam:PF12794"
FT DOMAIN 875..916
FT /note="Mechanosensitive ion channel transmembrane helices
FT 2/3"
FT /evidence="ECO:0000259|Pfam:PF21088"
FT DOMAIN 918..983
FT /note="Mechanosensitive ion channel MscS"
FT /evidence="ECO:0000259|Pfam:PF00924"
FT DOMAIN 991..1074
FT /note="Mechanosensitive ion channel MscS C-terminal"
FT /evidence="ECO:0000259|Pfam:PF21082"
FT COILED 121..158
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 196..238
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1107 AA; 123984 MW; 7D4F282F55C67C8F CRC64;
MRLIITFLMA WCLSWGAYAA TAPDSKQITQ ELEQAKAAKP AQPEVVEALQ SALNALEERK
GSLERIKQYQ EVIDNYPKLS ATLRAQLNNM RDEPRSVSPG MSNDALNQEI LQISSQLLDK
SRQAQQEQER AREIADSLNQ LPQQQTDARR QLNEIERRLG TLTGNTPLNQ AQNFALQSDS
ARLKALVDEL ELAQLSANNR QELARLRSEL AEKESQQLDA YLQALRNQLN SQRQLEAERA
LESTEQLAES SADLPKDIVA QFKINRELSA ALNQQAQRMD LVASQQRQAA SQTLQVRQAL
NTLREQSQWL GSSNLLGEAL RAQVARLPEM PKPQQLDTEM AQLRVQRLRY EDLLNKQPLL
RQIHQADGQP LTAEQNRILE AQLRTQRELL NSLLQGGDTL LLELTKLKVS NGQLEDALKE
VNEATHRYLF WTSDVRPMTI AWPLEIAQDL RRLISLDTFS QLGKASVMML TSKETILPLF
GALILVGCSI YSRRYFTRFL ERSAAKVGKV TQDHFWLTLR TLFWSILVAS PLPVLWMTLG
YGLREAWPYP LAVAIGDGVT ATVPLLWVVM ICATFARPNG LFIAHFGWPR ERVSRGMRYY
LMSIGLIVPL IMALMMFDNL DDREFSGSLG RLCFILICGA LAVVTLSLKK AGIPLYLNKE
GSGDNITNHM LWNMMIGAPL VAILASAVGY LATAQALLAR LETSVAIWFL LLVVYHVIRR
WMLIQRRRLA FDRAKHRRAE MLAQRARGEE EAHHHSSPEG AIEVDESEVD LDAISAQSLR
LVRSILMLIA LLSVIVLWSE IHSAFGFLEN ISLWDVTSTV QGVESLEPIT LGAVLIAILV
FIITTQLVRN LPALLELAIL QHLDLTPGTG YAITTITKYL LMLIGGLVGF SMIGIEWSKL
QWLVAALGVG LGFGLQEIFA NFISGLIILF EKPIRIGDTV TIRDLTGSVT KINTRATTIS
DWDRKEIIVP NKAFITEQFI NWSLSDSVTR VVLTIPAPAD ANSEEVTEIL LTAARRCSLV
IDNPAPEVFL VDLQQGIQIF ELRIYAAEMG HRMPLRHEIH QLILAGFHAH GIDMPFPPFQ
MRLESLNGKQ TGRTLTSAGK GRQAGSL
//
