ID S2F6T5_9PSED Unreviewed; 833 AA.
AC S2F6T5;
DT 18-SEP-2013, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2013, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=PG5_15330 {ECO:0000313|EMBL:EPA97804.1};
OS Pseudomonas sp. G5(2012).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1268068 {ECO:0000313|EMBL:EPA97804.1, ECO:0000313|Proteomes:UP000014290};
RN [1] {ECO:0000313|EMBL:EPA97804.1, ECO:0000313|Proteomes:UP000014290}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G5(2012) {ECO:0000313|Proteomes:UP000014290};
RA Park S., Lee J.-H., Cho Y.-J., Chun J., Hur H.-G.;
RT "Draft Genome Sequence of Pseudomonas sp. Strain G5, Isolated from a
RT Traditional Indigo Fermentation Dye Vat.";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EPA97804.1}.
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DR EMBL; APIO01000025; EPA97804.1; -; Genomic_DNA.
DR RefSeq; WP_020796508.1; NZ_APIO01000025.1.
DR AlphaFoldDB; S2F6T5; -.
DR PATRIC; fig|1268068.3.peg.1461; -.
DR eggNOG; COG0286; Bacteria.
DR Proteomes; UP000014290; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000313|EMBL:EPA97804.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 8..149
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 163..487
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT REGION 696..723
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 709..723
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 833 AA; 93681 MW; C96BA19B770C06B2 CRC64;
MPLTLPQLER HLFKAADILR GKMDASEFKE YIFGMLFLKR CSDMFEQRRE EVIRLEMEGG
KSKVQAQVSS EISRWYKIEG SFWVPPQSRY EFLLNDAHQN VGDFLNKALT GIETANTSLY
DVLEHIDFTR KVGQSKISDI KLRQLITHFG VFRLRNEDFE FPDLLGAAYE YLIGEFADSA
GKKGGEFYTP RSVVRMMVRL IKPELKHDIY DPCCGSGGML IAAKEYIDEH GEDGRKANLF
GQEFNGTVWS IAKMNMLLHG ISNADLHNED TLAEPQHVEG GELKHFDRVL TNPPFSIQWG
NTEKDADGKP AWKPKFEAER FSYGQVPLGA KKADMMFLQH MLAVTRDGGM VATVLPHGVL
FRGGEERIIR KGIIEDDLLE AVIGVAPNLF YGTSIPACIL VLRQRVQKGA NRVSGKPTER
RGKVLFINAD RELFEGRAQN YLLPEHIEKI VTTFDEFRAI DGFSAIVDYA TLKANDYNLN
IRRYADNSPP PEPHDVRAHL VGGIPKAEVA DKAGLFSAHG LNPMDLLVER EAKYLDFAPV
LDKRQALKPT IESNSGLVAK EAAIRTAFEE WWQTHSARIT ALSGQMDNAA SLVALRNELL
ASFSQSLETV GLLNPFQVRG IVAGFWYQTK YDFLTLMARG AKGVADAWRT SIVTALEDKA
SKESPLEHKL VKFLMSDFVE VITELEAKKA ELDSQIKAAS PKDVEGEDGE TVEAGEHEAD
EESVVDEAQL KAWKKELTAI KKQLKAKNDS FKQHINTAVK GLAPEAADDL LLTILHNDMQ
TIVERYMAAQ RKEIVASFEN WWDKYRMTLT EIEGKRDAAA KALQGFLKGL RYV
//
