ID S4T3C2_9INFA Unreviewed; 277 AA.
AC S4T3C2;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:AFT64755.1};
OS Influenza A virus (A/mallard/Sweden/99841/2009(H6N2)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=1229587 {ECO:0000313|EMBL:AFT64755.1};
RN [1] {ECO:0000313|EMBL:AFT64755.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/mallard/Sweden/99841/2009 {ECO:0000313|EMBL:AFT64755.1};
RX PubMed=23725694; DOI=10.1016/j.virol.2013.05.004;
RA Wille M., Tolf C., Avril A., Latorre-Margalef N., Wallerstrom S., Olsen B.,
RA Waldenstrom J.;
RT "Frequency and patterns of reassortment in natural influenza A virus
RT infection in a reservoir host.";
RL Virology 443:150-160(2013).
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; JX565863; AFT64755.1; -; Viral_cRNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 4: Predicted;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103}; Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Transferase {ECO:0000256|ARBA:ARBA00022484};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1..114
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AFT64755.1"
FT NON_TER 277
FT /evidence="ECO:0000313|EMBL:AFT64755.1"
SQ SEQUENCE 277 AA; 31573 MW; F175AB4E89BB0BAA CRC64;
AEMLANIDLK YFNESTRKKI EKIRPLLIDG TASLSPGMMM GMFNMLSTVL GVSILNLGQK
RYTKTTYWWD GLQSSDDFAL IVNAPNHEGI QAGVDRFYRT CKLVGINMSK KKSYVNRTGT
FEFTSFFYRY GFVANFSMEL PSFGVSGINE SADMSIGVTV IKNNMINNDL GPATAQMALQ
LFIKDYRYTY RCHRGDTQIQ TRRSFELKKL WEQTRSKAGL LVSDGGPNLY NIRNLHIPEV
CLKWELMDED YQGRLCNPLN PFVSHKEIES VNNAVVM
//
