ID S5STY3_RHIET Unreviewed; 369 AA.
AC S5STY3;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE SubName: Full=Electron transfer flavoprotein nitrogen fixation protein FixB {ECO:0000313|EMBL:AGS25417.1};
GN Name=fixB {ECO:0000313|EMBL:AGS25417.1};
GN ORFNames=REMIM1_PE00330 {ECO:0000313|EMBL:AGS25417.1};
OS Rhizobium etli bv. mimosae str. Mim1.
OG Plasmid pRetMIM1e {ECO:0000313|EMBL:AGS25417.1,
OG ECO:0000313|Proteomes:UP000015261}.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=1328306 {ECO:0000313|EMBL:AGS25417.1, ECO:0000313|Proteomes:UP000015261};
RN [1] {ECO:0000313|EMBL:AGS25417.1, ECO:0000313|Proteomes:UP000015261}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mim1 {ECO:0000313|EMBL:AGS25417.1,
RC ECO:0000313|Proteomes:UP000015261};
RC PLASMID=Plasmid pRetMIM1e {ECO:0000313|Proteomes:UP000015261};
RA Rogel M.A., Bustos P., Santamaria R.I., Gonzalez V., Romero D.,
RA Cevallos M.A., Lozano L., Ormeno-Orrillo E., Martinez-Romero E.;
RT "Genomic basis of Rhizobium etli symbiovar mimosae and its prevalence among
RT Phaseolus vulgaris nodule bacteria.";
RL Submitted (MAY-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May play a role in a redox process involved in nitrogen
CC fixation. {ECO:0000256|ARBA:ARBA00003554}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|PIRSR:PIRSR000089-1};
CC Note=Binds 1 FAD per dimer. {ECO:0000256|PIRSR:PIRSR000089-1};
CC -!- SUBUNIT: FixA and FixB form a heterodimer.
CC {ECO:0000256|ARBA:ARBA00011874}.
CC -!- SIMILARITY: Belongs to the ETF alpha-subunit/FixB family.
CC {ECO:0000256|ARBA:ARBA00005817}.
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DR EMBL; CP005955; AGS25417.1; -; Genomic_DNA.
DR RefSeq; WP_020923574.1; NC_021909.1.
DR AlphaFoldDB; S5STY3; -.
DR KEGG; rel:REMIM1_PE00330; -.
DR PATRIC; fig|1328306.3.peg.5839; -.
DR HOGENOM; CLU_034178_1_1_5; -.
DR Proteomes; UP000015261; Plasmid pRetMIM1e.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd01715; ETF_alpha; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR InterPro; IPR014730; ETF_a/b_N.
DR InterPro; IPR001308; ETF_a/FixB.
DR InterPro; IPR033947; ETF_alpha_N.
DR InterPro; IPR014731; ETF_asu_C.
DR InterPro; IPR018206; ETF_asu_C_CS.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR43153; ELECTRON TRANSFER FLAVOPROTEIN ALPHA; 1.
DR PANTHER; PTHR43153:SF1; ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR Pfam; PF01012; ETF; 1.
DR Pfam; PF00766; ETF_alpha; 1.
DR PIRSF; PIRSF000089; Electra_flavoP_a; 1.
DR SMART; SM00893; ETF; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR PROSITE; PS00696; ETF_ALPHA; 1.
PE 3: Inferred from homology;
KW Electron transport {ECO:0000256|ARBA:ARBA00022982};
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRSR:PIRSR000089-1};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231};
KW Plasmid {ECO:0000313|EMBL:AGS25417.1};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 32..223
FT /note="Electron transfer flavoprotein alpha/beta-subunit N-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00893"
FT BINDING 273..274
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000089-1"
FT BINDING 287..291
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000089-1"
FT BINDING 304..311
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000089-1"
FT BINDING 325
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000089-1"
SQ SEQUENCE 369 AA; 39581 MW; 936D6C14B8CD2BA7 CRC64;
MLSTNRESPP PAVGRAAMKK ELPEKFKAHR HVWVFIELER GQVHPVSFEL LGEGRKLADK
LGVQLAGVVL GPPGEFTWFA IAEAFAYGAD LAYLVEAPLL ADYRNEPFTK ALTELVTTYK
PEILLLGATT LGRDLAGSVA TTLLTGLTAD CTELDVDADG SLAATRPTFG GSLLCTIYTL
NCRPQMATVR PRVMAMPQRG NNQIGRVIQH DVPMIEEEIV TKVLGFLSDG LSATANLAYA
DVVVAGGLGL GAAENLNLVK KLAQTIGADY GCSRPLVQKG WMPADRQIGQ TGKTIRPKLY
IAAGISGAIQ HRVGVEGADL ILAINTDPNA PIFDFAHLGV VTDAIRLLPS LTELFTHRLS
PHSRDKLAN
//
