ID S8E0R6_9LAMI Unreviewed; 634 AA.
AC S8E0R6;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE RecName: Full=beta-galactosidase {ECO:0000256|ARBA:ARBA00012756};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
DE Flags: Fragment;
GN ORFNames=M569_05474 {ECO:0000313|EMBL:EPS69288.1};
OS Genlisea aurea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Lamiales; Lentibulariaceae; Genlisea.
OX NCBI_TaxID=192259 {ECO:0000313|EMBL:EPS69288.1, ECO:0000313|Proteomes:UP000015453};
RN [1] {ECO:0000313|EMBL:EPS69288.1, ECO:0000313|Proteomes:UP000015453}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23855885;
RA Leushkin E.V., Sutormin R.A., Nabieva E.R., Penin A.A., Kondrashov A.S.,
RA Logacheva M.D.;
RT "The miniature genome of a carnivorous plant Genlisea aurea contains a low
RT number of genes and short non-coding sequences.";
RL BMC Genomics 14:476-476(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EPS69288.1}.
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DR EMBL; AUSU01002191; EPS69288.1; -; Genomic_DNA.
DR AlphaFoldDB; S8E0R6; -.
DR OrthoDB; 5489808at2759; -.
DR Proteomes; UP000015453; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000015453}.
FT DOMAIN 10..335
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 378..504
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 549..608
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 158
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 241
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:EPS69288.1"
FT NON_TER 634
FT /evidence="ECO:0000313|EMBL:EPS69288.1"
SQ SEQUENCE 634 AA; 72033 MW; 1C49D362B9289EAE CRC64;
RKFEIKDDMF WKDNKPFRII GGDLHYFRVH PEYWEDRLLR AKALGLNTIQ TYVPWNLHEP
RKGELVFHGF ADIVSFLKLC EKLDLLVMLR AGPYICGEWD LGGFPSWLLT KDPPIRLRSS
DPAFIELVNS WWSILLPKIH PLVYRNGGSV VMIQIENEFG SFGDDKAYLH NLVVLARSHL
GSDVVLYTTD GGTRETLEKG TIMGDDVFSA VDFSTGDDPW PIFQLQKKFN PPGKSPPLSA
EFYTGWLTHW NEEIASTDAE FTATYLEKIL SMNGSVVLYM VHGGTNFGFY SGANTGVDES
DYKPDLTSYD YDAPIREYGD VKNAKYEALR KVIAKYGEVV SVPPDNEKTA YGRIKLEKQS
SLFDMISYRS HVLHSKNPIS MEAAFQQMFG FVLYSSEYVP RTNGSSTTLI ISIGFILQVH
DRAQVFISCP EKRPTYIGLI TRSSNRRAVD LPYLKCGQGK NKLLILVENM GHLNYGPYIF
DRKGILSSVY LDGREHYEWE MFPISLQNLN EDINPDIAAK IHRKGLAYTN VGYLTGMYSS
SADDLYQEPA FYVGHFHVER VGDTFISFRD WSKGIAFVNN NNIGRFWPSV GPQCSLYVPA
PFLREGTNTL VILELESPSS ELYVSSVDHP DFTC
//
