ID S9ZIL0_9RHOO Unreviewed; 745 AA.
AC S9ZIL0;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=Isocitrate dehydrogenase [NADP] {ECO:0000256|PIRNR:PIRNR009407};
DE EC=1.1.1.42 {ECO:0000256|PIRNR:PIRNR009407};
DE AltName: Full=Oxalosuccinate decarboxylase {ECO:0000256|PIRNR:PIRNR009407};
GN ORFNames=M622_05450 {ECO:0000313|EMBL:EPZ14426.1};
OS Thauera terpenica 58Eu.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC Thauera.
OX NCBI_TaxID=1348657 {ECO:0000313|EMBL:EPZ14426.1, ECO:0000313|Proteomes:UP000015455};
RN [1] {ECO:0000313|EMBL:EPZ14426.1, ECO:0000313|Proteomes:UP000015455}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=58Eu {ECO:0000313|EMBL:EPZ14426.1,
RC ECO:0000313|Proteomes:UP000015455};
RA Liu B., Frostegard A.H., Shapleigh J.P.;
RT "Draft genome sequence of Thauera terpenica.";
RL Submitted (JUN-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC Evidence={ECO:0000256|PIRNR:PIRNR009407};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC {ECO:0000256|PIRSR:PIRSR009407-3};
CC -!- SIMILARITY: Belongs to the monomeric-type IDH family.
CC {ECO:0000256|PIRNR:PIRNR009407}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EPZ14426.1}.
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DR EMBL; ATJV01000081; EPZ14426.1; -; Genomic_DNA.
DR RefSeq; WP_021250379.1; NZ_ATJV01000081.1.
DR AlphaFoldDB; S9ZIL0; -.
DR STRING; 1348657.M622_05450; -.
DR PATRIC; fig|1348657.5.peg.2980; -.
DR eggNOG; COG2838; Bacteria.
DR OrthoDB; 9807643at2; -.
DR Proteomes; UP000015455; Unassembled WGS sequence.
DR GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR InterPro; IPR004436; Isocitrate_DH_NADP_mono.
DR NCBIfam; TIGR00178; monomer_idh; 1.
DR PANTHER; PTHR36999; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR PANTHER; PTHR36999:SF1; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR Pfam; PF03971; IDH; 1.
DR PIRSF; PIRSF009407; IDH_monmr; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass {ECO:0000256|PIRNR:PIRNR009407};
KW Magnesium {ECO:0000256|PIRSR:PIRSR009407-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR009407-3};
KW NADP {ECO:0000256|PIRNR:PIRNR009407};
KW Oxidoreductase {ECO:0000256|PIRNR:PIRNR009407,
KW ECO:0000313|EMBL:EPZ14426.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000015455};
KW Tricarboxylic acid cycle {ECO:0000256|PIRNR:PIRNR009407}.
FT BINDING 134..141
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 147
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 352
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT BINDING 550
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 551
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT BINDING 555
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT SITE 257
FT /note="Critical for catalysis"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
FT SITE 422
FT /note="Critical for catalysis"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
SQ SEQUENCE 745 AA; 82657 MW; 28320ACAAE3598E0 CRC64;
MAEGKSKIIY TLTDEAPLLA TCAFLPIVRS FTEPTGIEVE TADISVAGRV LAEFPEYLSE
EQRVPDTLAE LGRLTLLPDT NIIKLPNISA SVAQLKVCIK ELQAKGYAIP DYPEDPKTDE
EKALRQRYGK CLGSAVNPVL REGNSDRRAP LAVKNYAKKN PHSMGEWKQW SQTHVSHMHH
GDFYHGEKSI TLDKARDVKM ELITASGKTI VLKPKVSLLD GEIIDSMFMS KKALCEFYER
ELDDCREAGI LFSLHVKATM MKVSHPIVFG HCVRIYYKDA FAKHGKLFDE LGINVNNGMV
DLYEKIKTLP ESKHDEIIRD LHACNEHRPE LAMVDSAKGI TNFHSPSDII VDASMPAMIR
QGGKMWGSDG KQYDSKCVMP ESTFARIYQE MINFCKWHGN FDPRTMGTVP NVGLMAQKAE
EYGSHDKTFE IAEAGVANIT DLATGEVLLA QNVEAGDIWR MCQVKDAPIR DWVKLAVTRA
RNSGMPAVFW LDPYRPHENE LIKKVHTYLK DHDTKGLEIH IMSQVRAMRY TLERVGRGLD
TISVTGNILR DYLTDLFPIL ELGTSAKMLS IVPLMNGGGM YETGAGGSAP KHVQQLVQEN
HLRWDSLGEF LALAVSFEDL GIKTGNNKAK ILARTLDAAT GKLLDENKSP SPKTGQLDNR
GSQFYLALFW AQALAAQTED AELAAHFVPL AKALSENETK IVDELNAVQG KPVDIGGYYK
ADAAKTIAVM RPSATLNAIL KAARA
//
