ID SAM50_BOVIN Reviewed; 469 AA.
AC Q2HJ55;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=Sorting and assembly machinery component 50 homolog;
GN Name=SAMM50;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Uterus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays a crucial role in the maintenance of the structure of
CC mitochondrial cristae and the proper assembly of the mitochondrial
CC respiratory chain complexes. Required for the assembly of TOMM40 into
CC the TOM complex. {ECO:0000250|UniProtKB:Q9Y512}.
CC -!- SUBUNIT: Associates with the mitochondrial contact site and cristae
CC organizing system (MICOS) complex, composed of at least MICOS10/MIC10,
CC CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26
CC and QIL1/MIC13 (By similarity). This complex was also known under the
CC names MINOS or MitOS complex (By similarity). The MICOS complex
CC associates with mitochondrial outer membrane proteins SAMM50, MTX1 and
CC MTX2 (together described as components of the mitochondrial outer
CC membrane sorting assembly machinery (SAM) complex) and DNAJC11,
CC mitochondrial inner membrane protein TMEM11 and with HSPA9 (By
CC similarity). The MICOS and SAM complexes together with DNAJC11 are part
CC of a large protein complex spanning both membranes termed the
CC mitochondrial intermembrane space bridging (MIB) complex (By
CC similarity). Interacts with IMMT/MIC60. Interacts with CHCHD3/MIC19 (By
CC similarity). Interacts with ARMC1 (By similarity).
CC {ECO:0000250|UniProtKB:Q9Y512}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000250|UniProtKB:Q9Y512}; Multi-pass membrane protein
CC {ECO:0000250}. Cytoplasm {ECO:0000250|UniProtKB:Q6AXV4}. Mitochondrion
CC {ECO:0000250|UniProtKB:Q9Y512}.
CC -!- DOMAIN: Its C-terminal part seems to contain many membrane-spanning
CC sided beta-sheets, that have the potential to adopt a transmembrane
CC beta-barrel type structure. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SAM50/omp85 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BT025460; ABF57416.1; -; mRNA.
DR EMBL; BC113305; AAI13306.1; -; mRNA.
DR RefSeq; NP_001040088.1; NM_001046623.1.
DR AlphaFoldDB; Q2HJ55; -.
DR SMR; Q2HJ55; -.
DR STRING; 9913.ENSBTAP00000073084; -.
DR PaxDb; 9913-ENSBTAP00000055147; -.
DR DNASU; 618777; -.
DR Ensembl; ENSBTAT00000063243.2; ENSBTAP00000055147.2; ENSBTAG00000045957.2.
DR GeneID; 618777; -.
DR KEGG; bta:618777; -.
DR CTD; 25813; -.
DR VEuPathDB; HostDB:ENSBTAG00000045957; -.
DR VGNC; VGNC:34278; SAMM50.
DR eggNOG; KOG2602; Eukaryota.
DR GeneTree; ENSGT00390000011355; -.
DR InParanoid; Q2HJ55; -.
DR OMA; SGIWRQI; -.
DR OrthoDB; 46657at2759; -.
DR Reactome; R-BTA-9013404; RAC2 GTPase cycle.
DR Proteomes; UP000009136; Chromosome 5.
DR Bgee; ENSBTAG00000045957; Expressed in digestive system secreted substance and 105 other cell types or tissues.
DR ExpressionAtlas; Q2HJ55; baseline and differential.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042407; P:cristae formation; ISS:UniProtKB.
DR GO; GO:0033108; P:mitochondrial respiratory chain complex assembly; ISS:UniProtKB.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IBA:GO_Central.
DR Gene3D; 3.10.20.310; membrane protein fhac; 1.
DR Gene3D; 2.40.160.50; membrane protein fhac: a member of the omp85/tpsb transporter family; 1.
DR InterPro; IPR000184; Bac_surfAg_D15.
DR InterPro; IPR039910; D15-like.
DR InterPro; IPR034746; POTRA.
DR PANTHER; PTHR12815:SF18; SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG; 1.
DR PANTHER; PTHR12815; SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER; 1.
DR Pfam; PF01103; Omp85; 1.
DR PROSITE; PS51779; POTRA; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Membrane; Methylation; Mitochondrion;
KW Mitochondrion outer membrane; Reference proteome; Transmembrane;
KW Transmembrane beta strand.
FT CHAIN 1..469
FT /note="Sorting and assembly machinery component 50 homolog"
FT /id="PRO_0000383680"
FT DOMAIN 45..125
FT /note="POTRA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01115"
FT MOD_RES 255
FT /note="N6-methyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y512"
SQ SEQUENCE 469 AA; 52042 MW; 859F52F5D193A31D CRC64;
MGTVHARSLE PLPASGPDFG ALGEEAEFVE VEPEAKQEIL ENKDVVVQHV HFDGLGRTKD
DIIMYEIRDV FKAKNLIEVM RKSHEAREKL LRLGIFRQVD VLIDTCQGDD ALPNGLDVTF
EVTELRRLTG SYNTMVGNNE GSMVLGLKLP NLLGRAEKVT FQFSYGTKET SYGLSFFKPQ
PGNFDRNFSV NLYKVTGQFP WSSLRETDRG VSAEYSFPTW KTSHTVKWEG VWRELGCLSR
VASFAVRKES GHSLKSSLSH SMVIDSRNSS ILPKRGALLK VNQELAGYTG GDVSFLKEDF
ELQLNKQLIL DTVFSASLWG GMLVPMGDKP SSIADRFYLG GPTSVRGFSM HSVGPQSEGD
YLGGEAYWAG GLHLYTPLPF RPGQGGFGEL FRTHFFLNAG NLCNLNYGEG PKAHIRKLAE
CIRWSYGAGI VLRLGNIARL ELNYCVPMGV QRGDRICDGV QFGAGIRFL
//
