ID SMT2_ORYSJ Reviewed; 363 AA.
AC O82427;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 2.
DT 27-MAR-2024, entry version 125.
DE RecName: Full=24-methylenesterol C-methyltransferase 2;
DE Short=24-sterol C-methyltransferase 2;
DE Short=Sterol-C-methyltransferase 2;
DE EC=2.1.1.143;
GN Name=Smt2-1; OrderedLocusNames=Os03g0136200, LOC_Os03g04340;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Callus;
RX PubMed=9746350; DOI=10.1046/j.1432-1327.1998.2560088.x;
RA Bouvier-Nave P., Husselstein T., Benveniste P.;
RT "Two families of sterol methyltransferases are involved in the first and
RT the second methylation steps of plant sterol biosynthesis.";
RL Eur. J. Biochem. 256:88-96(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Catalyzes the methyl transfer from S-adenosyl-methionine to
CC the methylene group of 24-methylene lophenol to form 24-ethylidene
CC lophenol. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=24-methylidenelophenol + S-adenosyl-L-methionine = (Z)-24-
CC ethylidenelophenol + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:21044, ChEBI:CHEBI:15378, ChEBI:CHEBI:29107,
CC ChEBI:CHEBI:33203, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789;
CC EC=2.1.1.143;
CC -!- PATHWAY: Steroid biosynthesis; sterol biosynthesis.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Erg6/SMT family. {ECO:0000255|PROSITE-ProRule:PRU01022}.
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DR EMBL; AF042333; AAC34989.1; -; mRNA.
DR EMBL; AC118132; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP014959; BAS82182.1; -; Genomic_DNA.
DR RefSeq; XP_015630743.1; XM_015775257.1.
DR AlphaFoldDB; O82427; -.
DR SMR; O82427; -.
DR STRING; 39947.O82427; -.
DR PaxDb; 39947-O82427; -.
DR EnsemblPlants; Os03t0136200-01; Os03t0136200-01; Os03g0136200.
DR GeneID; 4331540; -.
DR Gramene; Os03t0136200-01; Os03t0136200-01; Os03g0136200.
DR KEGG; osa:4331540; -.
DR eggNOG; KOG1269; Eukaryota.
DR HOGENOM; CLU_039068_5_2_1; -.
DR InParanoid; O82427; -.
DR OMA; ECTRIHE; -.
DR OrthoDB; 716003at2759; -.
DR PlantReactome; R-OSA-1119370; Sterol biosynthesis.
DR UniPathway; UPA00766; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; O82427; baseline and differential.
DR Genevisible; O82427; OS.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030797; F:24-methylenesterol C-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR013216; Methyltransf_11.
DR InterPro; IPR030384; MeTrfase_SMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR013705; Sterol_MeTrfase_C.
DR PANTHER; PTHR44742; -; 1.
DR PANTHER; PTHR44742:SF2; 24-METHYLENESTEROL C-METHYLTRANSFERASE 2; 1.
DR Pfam; PF08241; Methyltransf_11; 1.
DR Pfam; PF08498; Sterol_MT_C; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51685; SAM_MT_ERG6_SMT; 1.
PE 2: Evidence at transcript level;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Methyltransferase;
KW Reference proteome; S-adenosyl-L-methionine; Steroid biosynthesis;
KW Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..363
FT /note="24-methylenesterol C-methyltransferase 2"
FT /id="PRO_0000124804"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 214
FT /note="D -> E (in Ref. 1; AAC34989)"
FT /evidence="ECO:0000305"
FT CONFLICT 284
FT /note="L -> V (in Ref. 1; AAC34989)"
FT /evidence="ECO:0000305"
FT CONFLICT 291..292
FT /note="AL -> GF (in Ref. 1; AAC34989)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 363 AA; 40619 MW; ED7398EAC1D1B000 CRC64;
MEAATMAWTA AGVGMALVYW FVWVMGAAEV KGKRAVDLKM GSITNDKVKD KYTQYWSFFR
RPKETATTEA SAEKVPAFVD TFYNLVTDIY EWGWGQSFHF SPSLPGRSHR EATRVHEERV
ADLLQAKPGH RLLDVGCGVG GPMRAIAAHS GSNVVGITIN EYQVNRARAH NRKAGLDSRC
EVVCGNFLSM PFSDASFDGA YSIEATCHAP RLQDVYGEVF RVLKPGGLYV SYEWVTTSLY
RADNPEHVEA IHGIERGDAL PGLRRQDEIA SIAKEVGFEV LKELDLALPP ALPWWTRLKM
GRIAYWRNSL VVRVLTMLRI APKGVCEVHE MLYETAQHLT RGGETGIFTP MHMVLLRKPV
ESK
//
