ID SNX41_YEAST Reviewed; 625 AA.
AC Q04053; D6VT55;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 169.
DE RecName: Full=Sorting nexin-41;
GN Name=SNX41; OrderedLocusNames=YDR425W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, AND INTERACTION WITH SNX4.
RX PubMed=12554655; DOI=10.1093/emboj/cdg062;
RA Hettema E.H., Lewis M.J., Black M.W., Pelham H.R.B.;
RT "Retromer and the sorting nexins Snx4/41/42 mediate distinct retrieval
RT pathways from yeast endosomes.";
RL EMBO J. 22:548-557(2003).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Involved in proper sorting of the v-SNARE protein SNC1.
CC {ECO:0000269|PubMed:12554655}.
CC -!- SUBUNIT: Binds to SNX4.
CC -!- INTERACTION:
CC Q04053; P47057: SNX4; NbExp=7; IntAct=EBI-30464, EBI-17610;
CC -!- SUBCELLULAR LOCATION: Prevacuolar compartment
CC {ECO:0000269|PubMed:14562095}. Endosome {ECO:0000269|PubMed:14562095}.
CC Endosome membrane {ECO:0000269|PubMed:14562095}; Peripheral membrane
CC protein {ECO:0000269|PubMed:14562095}; Cytoplasmic side
CC {ECO:0000269|PubMed:14562095}. Note=Perivacuolar punctate structures
CC and endosome.
CC -!- DOMAIN: The PX domain binds to phosphatidylinositol 3-phosphate which
CC is necessary for peripheral membrane localization to the punctate
CC structures. {ECO:0000250}.
CC -!- MISCELLANEOUS: Present with 450 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the sorting nexin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U33007; AAB64858.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12265.1; -; Genomic_DNA.
DR PIR; S69707; S69707.
DR RefSeq; NP_010713.1; NM_001180733.1.
DR AlphaFoldDB; Q04053; -.
DR BioGRID; 32484; 126.
DR ComplexPortal; CPX-1378; SNX4-SNX41 sorting nexin complex.
DR DIP; DIP-3920N; -.
DR IntAct; Q04053; 17.
DR MINT; Q04053; -.
DR STRING; 4932.YDR425W; -.
DR iPTMnet; Q04053; -.
DR MaxQB; Q04053; -.
DR PaxDb; 4932-YDR425W; -.
DR PeptideAtlas; Q04053; -.
DR EnsemblFungi; YDR425W_mRNA; YDR425W; YDR425W.
DR GeneID; 852035; -.
DR KEGG; sce:YDR425W; -.
DR AGR; SGD:S000002833; -.
DR SGD; S000002833; SNX41.
DR VEuPathDB; FungiDB:YDR425W; -.
DR eggNOG; KOG2273; Eukaryota.
DR HOGENOM; CLU_014456_3_0_1; -.
DR InParanoid; Q04053; -.
DR OMA; DFKDPWG; -.
DR OrthoDB; 5475877at2759; -.
DR BioCyc; YEAST:G3O-29966-MONOMER; -.
DR BioGRID-ORCS; 852035; 0 hits in 10 CRISPR screens.
DR PRO; PR:Q04053; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q04053; Protein.
DR GO; GO:0010009; C:cytoplasmic side of endosome membrane; IDA:ComplexPortal.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0035091; F:phosphatidylinositol binding; IDA:SGD.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:SGD.
DR GO; GO:0006886; P:intracellular protein transport; IDA:ComplexPortal.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IDA:ComplexPortal.
DR CDD; cd06867; PX_SNX41_42; 1.
DR Gene3D; 3.30.1520.10; Phox-like domain; 1.
DR InterPro; IPR001683; PX_dom.
DR InterPro; IPR036871; PX_dom_sf.
DR InterPro; IPR044106; PX_Snx41/Atg20.
DR PANTHER; PTHR46979; SORTING NEXIN-41; 1.
DR PANTHER; PTHR46979:SF2; SORTING NEXIN-41; 1.
DR Pfam; PF00787; PX; 1.
DR SMART; SM00312; PX; 1.
DR SUPFAM; SSF64268; PX domain; 1.
DR PROSITE; PS50195; PX; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endosome; Lipid-binding; Membrane; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..625
FT /note="Sorting nexin-41"
FT /id="PRO_0000213834"
FT DOMAIN 98..235
FT /note="PX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147"
FT REGION 1..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 437..469
FT /evidence="ECO:0000255"
FT COILED 539..563
FT /evidence="ECO:0000255"
FT COMPBIAS 8..23
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..88
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 153
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
FT BINDING 155
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
FT BINDING 179
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
FT BINDING 202
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
SQ SEQUENCE 625 AA; 70741 MW; B9A064EDE0C52085 CRC64;
MDYNIFEAVH EQQSSTSDMD LSEEDNNPFV GTHHLYASGI GTTIGEARPE NENSPPSSSS
LPSSPAHSSS AGSSRASTSS STSSHAVVEA DAETEPFVSL SMSTTATISK FTPHDMNGTQ
QIQIIDAGDF KDPWGKHAIG YVILYENNKI IRRYSEFHSL RQSLTRLLPT IIIPPIPSKH
SLLKYIWSPI NAANDSKIIS TRKKMLNSFL SNCLNIQEIS NDIVFQKFLN PEFNWKDVLS
SSPIIILPLN NLLAPPLSPT KPSPLHSILP IPSNSSLRNY NSIWQQHITV KSHNEISNLP
TEILQNESQF THIENLFQNY KRIITHLLKN IRSNKSHFHS LSTYFAELGA YYNAFSLEND
ITMPNSLRES ENNSNNPMME IISHIEKTGH SFDVIYISSE ILIEKYTSIL EDPINELLQF
LNESFKVLNF KKLKFLQFKI LERLIIEKET KLSSLTEIEN QLQKINESLT RSTILTDENY
KDTKAADLTF VKKDVRSLSK SSSNSSSSGH QNEIHIGASK LNYKTSTPTM NLNKLEIKQL
TEQERSKQIK QLNQDLSKLK DCLSICISDM LEINNSSYNS LMHTYNHINL TIGKILKLFA
ASFKAWIKEC LKNWKLAKLQ IDEAL
//
