ID SRF4_ARATH Reviewed; 687 AA.
AC Q6R2K2; Q9LK66;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 139.
DE RecName: Full=Protein STRUBBELIG-RECEPTOR FAMILY 4;
DE AltName: Full=Leucine-rich repeat receptor kinase-like protein SRF4;
DE Flags: Precursor;
GN Name=SRF4; OrderedLocusNames=At3g13065; ORFNames=MGH6.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=17397538; DOI=10.1186/1471-2229-7-16;
RA Eyueboglu B., Pfister K., Haberer G., Chevalier D., Fuchs A., Mayer K.F.X.,
RA Schneitz K.;
RT "Molecular characterisation of the STRUBBELIG-RECEPTOR FAMILY of genes
RT encoding putative leucine-rich repeat receptor-like kinases in Arabidopsis
RT thaliana.";
RL BMC Plant Biol. 7:16-16(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Acts as a direct positive regulator of leaf size but not leaf
CC shape. {ECO:0000269|PubMed:17397538}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, roots, stems, leaves,
CC flowers and siliques. {ECO:0000269|PubMed:17397538}.
CC -!- DOMAIN: The protein kinase domain is predicted to be catalytically
CC inactive.
CC -!- DISRUPTION PHENOTYPE: Plants show a reduction in leaf size, but no
CC apparent defect in pollen development or fertility.
CC {ECO:0000269|PubMed:17397538}.
CC -!- MISCELLANEOUS: Cannot functionally replace STRUBBELIG.
CC -!- MISCELLANEOUS: Over-expression of SRF4 led to an increased leaf surface
CC and to male-sterility in both cv. Landsberg and cv. Columbia.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01397.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY518289; AAR99872.1; -; mRNA.
DR EMBL; AP000375; BAB01397.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AB026645; BAB01397.1; JOINED; Genomic_DNA.
DR EMBL; CP002686; AEE75289.1; -; Genomic_DNA.
DR RefSeq; NP_566444.4; NM_112145.4.
DR AlphaFoldDB; Q6R2K2; -.
DR SMR; Q6R2K2; -.
DR BioGRID; 5828; 19.
DR IntAct; Q6R2K2; 19.
DR STRING; 3702.Q6R2K2; -.
DR GlyCosmos; Q6R2K2; 2 sites, No reported glycans.
DR PaxDb; 3702-AT3G13065-1; -.
DR ProteomicsDB; 226726; -.
DR EnsemblPlants; AT3G13065.1; AT3G13065.1; AT3G13065.
DR GeneID; 820494; -.
DR Gramene; AT3G13065.1; AT3G13065.1; AT3G13065.
DR KEGG; ath:AT3G13065; -.
DR Araport; AT3G13065; -.
DR TAIR; AT3G13065; SRF4.
DR eggNOG; ENOG502QSZ9; Eukaryota.
DR HOGENOM; CLU_000288_92_2_1; -.
DR InParanoid; Q6R2K2; -.
DR OMA; YFFESAH; -.
DR OrthoDB; 466261at2759; -.
DR PhylomeDB; Q6R2K2; -.
DR PRO; PR:Q6R2K2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q6R2K2; baseline and differential.
DR Genevisible; Q6R2K2; AT.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004672; F:protein kinase activity; IEA:InterPro.
DR Gene3D; 3.80.10.10; Ribonuclease Inhibitor; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR013210; LRR_N_plant-typ.
DR InterPro; IPR046959; PRK1-6/SRF4-like.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR PANTHER; PTHR48007; LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1; 1.
DR PANTHER; PTHR48007:SF13; PROTEIN STRUBBELIG-RECEPTOR FAMILY 4; 1.
DR Pfam; PF00560; LRR_1; 3.
DR Pfam; PF08263; LRRNT_2; 1.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR PRINTS; PR00019; LEURICHRPT.
DR SUPFAM; SSF52058; L domain-like; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Glycoprotein; Leucine-rich repeat; Membrane;
KW Nucleotide-binding; Receptor; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..687
FT /note="Protein STRUBBELIG-RECEPTOR FAMILY 4"
FT /id="PRO_0000311844"
FT TOPO_DOM 26..278
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..687
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 96..118
FT /note="LRR 1"
FT REPEAT 119..140
FT /note="LRR 2"
FT REPEAT 142..165
FT /note="LRR 3"
FT REPEAT 166..189
FT /note="LRR 4"
FT REPEAT 190..212
FT /note="LRR 5"
FT REPEAT 213..235
FT /note="LRR 6"
FT DOMAIN 415..686
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 239..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 310..329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 344..365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 378..398
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 421..429
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 443
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT CARBOHYD 187
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 263
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 687 AA; 74911 MW; C4321C08D24AB97A CRC64;
MGPNLQRIVL VFIACFGIFT SVVLAKTDSQ DVSALNDAYK SMNSPSKLKG WSSSGGDPCG
DSWDGITCKG SSVTEIKVSG RGLSGSLGYQ LGNLKSLTYL DVSKNNLNGN LPYQLPDKLT
YLDGSENDFN GNVPYSVSLM NDLSYLNLGR NNLNGELSDM FQKLPKLETI DLSSNQLTGK
LPQSFANLTG LKTLHLQENQ FKGSINALRD LPQIDDVNVA NNQFTGWIPN ELKNIGNLET
GGNKWSSGRA PSPPPGTRHI DRNSSGGGGG SSKALTLGVI IAVSSIGGLI LFAGLIALIS
RRKNSNDSSH FFDDEKGTNR SKPLFTPQSS QMLQFDNMEE FKNQKTVDSN TSLETKPSVK
RTSSVSFKNS PTFHLIPSTQ VAATPDRSST SQDSPDTRGV KAFSLADLQN TASCFSPNRL
LGEGTIGRVY KAKFQDGRKF AVKEIDSSLL GKGNPEEFSH IVSSISSIHH KNMAELVGYC
SEQGRNMLVY EYFTSGSLHR FLHLSDDFSK PLTWNTRIRI ALGTAKAIEY LHETCSPPLV
HKNIKSSNIL LDNELNPRLS DYGLANFHHR TSQNLGVGYN APECTDPSAY TQKSDVYSFG
VVMLELLTGR KPYDSGRPKA EQSLVRWAKP QLKDMDTLDE MVDPALCGLY APESVSSFAD
IVSICVMTEP GLRPPVSNVV EALKRLV
//
