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Database: UniProt
Entry: SUN4_YEAST
LinkDB: SUN4_YEAST
Original site: SUN4_YEAST 
ID   SUN4_YEAST              Reviewed;         420 AA.
AC   P53616; A2TBM4; D6W1B4; Q6B1F2; Q870H0;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   29-OCT-2014, entry version 100.
DE   RecName: Full=Probable secreted beta-glucosidase SUN4;
DE            EC=3.2.1.-;
DE   AltName: Full=Septation protein SUN4;
DE   AltName: Full=Soluble cell wall protein 3;
DE   Flags: Precursor;
GN   Name=SUN4; Synonyms=SCW3; OrderedLocusNames=YNL066W;
GN   ORFNames=N2411, YNL2411W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S288c / FY1676;
RX   PubMed=8533472; DOI=10.1002/yea.320111008;
RA   Bergez P., Doignon F., Crouzet M.;
RT   "The sequence of a 44 420 bp fragment located on the left arm of
RT   chromosome XIV from Saccharomyces cerevisiae.";
RL   Yeast 11:967-974(1995).
RN   [2]
RP   ERRATUM.
RX   PubMed=8904343;
RX   DOI=10.1002/(SICI)1097-0061(19960315)12:3<297::AID-YEA940>3.0.CO;2-D;
RA   Bergez P., Doignon F., Crouzet M.;
RL   Yeast 12:297-297(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=8701611;
RX   DOI=10.1002/(SICI)1097-0061(19960330)12:4<391::AID-YEA921>3.3.CO;2-E;
RA   Poehlmann R., Philippsen P.;
RT   "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV
RT   reveals 12 new open reading frames (ORFs) and an ancient duplication
RT   of six ORFs.";
RL   Yeast 12:391-402(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F.,
RA   Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M.,
RA   Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N.,
RA   Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D.,
RA   Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A.,
RA   Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A.,
RA   Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C.,
RA   Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M.,
RA   Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J.,
RA   Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L.,
RA   Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M.,
RA   Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P.,
RA   Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A.,
RA   Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV
RT   and its evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
RA   Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and
RT   now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
RA   Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
RA   Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
RA   Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-
RT   encoding clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-92.
RC   STRAIN=ATCC 204508 / S288c;
RA   Zhang Z., Dietrich F.S.;
RT   "YNL066W(SUN4) mRNA.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [8]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-66.
RC   STRAIN=ATCC 201390 / BY4743;
RX   PubMed=17244705; DOI=10.1073/pnas.0610354104;
RA   Juneau K., Palm C., Miranda M., Davis R.W.;
RT   "High-density yeast-tiling array reveals previously undiscovered
RT   introns and extensive regulation of meiotic splicing.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007).
RN   [9]
RP   PROTEIN SEQUENCE OF 25-35, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 96099 / S288c / SEY6210;
RX   PubMed=9748433;
RA   Cappellaro C., Mrsa V., Tanner W.;
RT   "New potential cell wall glucanases of Saccharomyces cerevisiae and
RT   their involvement in mating.";
RL   J. Bacteriol. 180:5030-5037(1998).
RN   [10]
RP   FUNCTION.
RX   PubMed=10870102;
RX   DOI=10.1002/1097-0061(200007)16:10<905::AID-YEA584>3.0.CO;2-1;
RA   Mouassite M., Camougrand N.M., Schwob E., Demaison G., Laclau M.,
RA   Guerin M.G.;
RT   "The 'SUN' family: yeast SUN4/SCW3 is involved in cell septation.";
RL   Yeast 16:905-919(2000).
RN   [11]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [12]
RP   SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE, AND FUNCTION.
RX   PubMed=24040106; DOI=10.1371/journal.pone.0073882;
RA   Kuznetsov E., Kucerova H., Vachova L., Palkova Z.;
RT   "SUN family proteins Sun4p, Uth1p and Sim1p are secreted from
RT   Saccharomyces cerevisiae and produced dependently on oxygen level.";
RL   PLoS ONE 8:E73882-E73882(2013).
CC   -!- FUNCTION: Involved in the remodeling of the cell wall during the
CC       various phases of yeast culture development and under various
CC       environmental conditions and plays a role in septation.
CC       {ECO:0000269|PubMed:10870102, ECO:0000269|PubMed:24040106}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC       {ECO:0000269|PubMed:24040106, ECO:0000269|PubMed:9748433}.
CC   -!- INDUCTION: Expression is decreased during transition to slow
CC       growing or stationary phases. {ECO:0000269|PubMed:24040106}.
CC   -!- PTM: Glycosylated.
CC   -!- DISRUPTION PHENOTYPE: Leads to increased resistance to zymolyase
CC       treatment. {ECO:0000269|PubMed:24040106}.
CC   -!- MISCELLANEOUS: Present with 24700 molecules/cell in log phase SD
CC       medium. {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the SUN family. {ECO:0000305}.
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DR   EMBL; U12141; AAA99645.1; -; Genomic_DNA.
DR   EMBL; X86470; CAA60196.1; -; Genomic_DNA.
DR   EMBL; Z71342; CAA95939.1; -; Genomic_DNA.
DR   EMBL; AY693128; AAT93147.1; -; Genomic_DNA.
DR   EMBL; AY245796; AAP04346.1; -; mRNA.
DR   EMBL; EF123127; ABM97471.1; -; mRNA.
DR   EMBL; BK006947; DAA10480.1; -; Genomic_DNA.
DR   PIR; S53916; S53916.
DR   RefSeq; NP_014333.1; NM_001182904.1.
DR   ProteinModelPortal; P53616; -.
DR   BioGrid; 35757; 16.
DR   DIP; DIP-4365N; -.
DR   MINT; MINT-487167; -.
DR   STRING; 4932.YNL066W; -.
DR   MaxQB; P53616; -.
DR   PaxDb; P53616; -.
DR   EnsemblFungi; YNL066W; YNL066W; YNL066W.
DR   GeneID; 855659; -.
DR   KEGG; sce:YNL066W; -.
DR   CYGD; YNL066w; -.
DR   SGD; S000005010; SUN4.
DR   eggNOG; NOG83991; -.
DR   GeneTree; ENSGT00390000002934; -.
DR   HOGENOM; HOG000180870; -.
DR   InParanoid; P53616; -.
DR   OMA; MHETEAR; -.
DR   OrthoDB; EOG79PK14; -.
DR   BioCyc; YEAST:G3O-33096-MONOMER; -.
DR   NextBio; 979920; -.
DR   Genevestigator; P53616; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
DR   GO; GO:0005759; C:mitochondrial matrix; TAS:SGD.
DR   GO; GO:0015926; F:glucosidase activity; ISS:SGD.
DR   GO; GO:0000917; P:barrier septum assembly; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0007005; P:mitochondrion organization; TAS:SGD.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR005556; SUN.
DR   Pfam; PF03856; SUN; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cell cycle; Cell division; Cell wall;
KW   Cell wall biogenesis/degradation; Complete proteome;
KW   Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Septation;
KW   Signal.
FT   SIGNAL        1     24       {ECO:0000269|PubMed:9748433}.
FT   CHAIN        25    420       Probable secreted beta-glucosidase SUN4.
FT                                /FTId=PRO_0000033466.
FT   COMPBIAS     31    148       Ser/Thr-rich.
FT   COMPBIAS    336    339       Poly-Ser.
FT   CARBOHYD    395    395       N-linked (GlcNAc...). {ECO:0000255}.
FT   CONFLICT    299    299       L -> S (in Ref. 6; AAT93147).
FT                                {ECO:0000305}.
SQ   SEQUENCE   420 AA;  43442 MW;  F1FB6CD46F2CDA13 CRC64;
     MKLSATTLTA ASLIGYSTIV SALPYAADID TGCTTTAHGS HQHKRAVAVT YVYETVTVDK
     NGQTVTPTST EASSTVASTT TLISESSVTK SSSKVASSSE STEQIATTSS SAQTTLTSSE
     TSTSESSVPI STSGSASTSS AASSATGSIY GDLADFSGPY EKFEDGTIPC GQFPSGQGVI
     PISWLDEGGW SGVENTDTST GGSCKEGSYC SYACQPGMSK TQWPSDQPSD GRSIGGLLCK
     DGYLYRSNTD TDYLCEWGVD AAYVVSELSN DVAICRTDYP GTENMVIPTY VQAGDSLPLT
     VVDQDTYYTW QGLKTSAQYY VNNAGISVED ACVWGSSSSG VGNWAPLNFG AGSSDGVAYL
     SLIPNPNNGN ALNFNVKIVA ADDSSTVNGE CIYENGSFSG GSDGCTVSVT AGKAKFVLYN
//
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