ID T0VF18_LACLC Unreviewed; 428 AA.
AC T0VF18;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
DE Flags: Fragment;
GN ORFNames=LLT3_15535 {ECO:0000313|EMBL:EQC94407.1};
OS Lactococcus cremoris subsp. cremoris TIFN3.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus; Lactococcus cremoris subsp. cremoris.
OX NCBI_TaxID=1234873 {ECO:0000313|EMBL:EQC94407.1, ECO:0000313|Proteomes:UP000015664};
RN [1] {ECO:0000313|EMBL:EQC94407.1, ECO:0000313|Proteomes:UP000015664}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TIFN3 {ECO:0000313|EMBL:EQC94407.1,
RC ECO:0000313|Proteomes:UP000015664};
RX PubMed=23823494; DOI=10.1038/ismej.2013.108;
RA Erkus O., de Jager V.C., Spus M., van Alen-Boerrigter I.J.,
RA van Rijswijck I.M., Hazelwood L., Janssen P.W., van Hijum S.A.,
RA Kleerebezem M., Smid E.J.;
RT "Multifactorial diversity sustains microbial community stability.";
RL ISME J. 7:2126-2136(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EQC94407.1}.
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DR EMBL; ATBE01000305; EQC94407.1; -; Genomic_DNA.
DR AlphaFoldDB; T0VF18; -.
DR Proteomes; UP000015664; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Endonuclease {ECO:0000313|EMBL:EQC94407.1};
KW Hydrolase {ECO:0000313|EMBL:EQC94407.1};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Nuclease {ECO:0000313|EMBL:EQC94407.1};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 5..157
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 169..426
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT NON_TER 428
FT /evidence="ECO:0000313|EMBL:EQC94407.1"
SQ SEQUENCE 428 AA; 48603 MW; 2813F79DFC3BA0B2 CRC64;
MATGLNQQLW ASADILRGKM DASEYKNYLL GLIFYKYLSD AQLREVYEQE NGKTDTFPER
STQYAGFMEW YEEDKDDLIE NIQPKQGYFI QPDQLFYHYR IKADNYEFNL TDLQAGFNEL
ERQGEEFSGL FADIDLNSTK LGSNAQQRNV TITEVLRALD EIDLFEHNGD VIGDAYEYLI
GMFAAGAGKK AGEFYTPQAV SRIMSEITSI GQESRAPFHI YDPAMGSGSL MLNIRRYLIN
PNQVHYHGQE LNTTTFNLAR MNLILHGVDK ERMNLNNGDT LDADWPSEEP YQFDSVVMNP
PYSAKWSAAD KFLSDPRFER FGKLAPKSKA DFAFLLHGFY HLKESGTMGI VLPHGVLFRG
AAEGTIRQAL LEMGAIDAVI GLPANIFFGT SIPTTVIILK KNRSRRDVLF IDASQDFEKQ
KNQNVLLD
//
