ID T0VKE7_LACLC Unreviewed; 1162 AA.
AC T0VKE7;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 24-JAN-2024, entry version 49.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=LLT3_13190 {ECO:0000313|EMBL:EQC96347.1};
OS Lactococcus cremoris subsp. cremoris TIFN3.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus; Lactococcus cremoris subsp. cremoris.
OX NCBI_TaxID=1234873 {ECO:0000313|EMBL:EQC96347.1, ECO:0000313|Proteomes:UP000015664};
RN [1] {ECO:0000313|EMBL:EQC96347.1, ECO:0000313|Proteomes:UP000015664}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TIFN3 {ECO:0000313|EMBL:EQC96347.1,
RC ECO:0000313|Proteomes:UP000015664};
RX PubMed=23823494; DOI=10.1038/ismej.2013.108;
RA Erkus O., de Jager V.C., Spus M., van Alen-Boerrigter I.J.,
RA van Rijswijck I.M., Hazelwood L., Janssen P.W., van Hijum S.A.,
RA Kleerebezem M., Smid E.J.;
RT "Multifactorial diversity sustains microbial community stability.";
RL ISME J. 7:2126-2136(2013).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EQC96347.1}.
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DR EMBL; ATBE01000001; EQC96347.1; -; Genomic_DNA.
DR AlphaFoldDB; T0VKE7; -.
DR PATRIC; fig|1234873.3.peg.15; -.
DR Proteomes; UP000015664; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 623..784
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 805..959
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1162 AA; 133678 MW; 75EE9B0F53C483D6 CRC64;
MNIIEFFDQN WQLQKWQQGF SKRERTLLTG LSGTAKSLVM ANAYENVAEK YIIVTDSQFH
ANELYDELST LLGEEKVFQF FSDDNIYAEF ALASKDRVAY RLEALNFLLD EKSTGFLVVP
FLALRSYLPS PENFLENYLL LTSGDEYDLS NLVNLLSNAG YEKTQRVMTP GEFSMRGDIV
DIYTLDAENP VRLEFFGDEV DTIRSFDVES QRSLTSLERL EIYPASDFIL TDNEFDKGAK
SLTEMTNLLT DPSAKSYMEE VISAAQNHYY HKDLRKFAEY FYDKKTSLLN YFPKNVQIFI
DDFQKVNEIN NKLEMELADF ILSEKAMERG FEGQTYLLDT MAKVRNYKPA TFFSNFQKGL
GNLRFEQLYN FKQHSMQQFF GQLELFYTEV ERFIKQEFTV VLAVSSEKLR KSLHELDLSL
QEVDRESIQV GKVNLIDLQL SNGFNFLDEK LVVMTEHEIF GKMRKKKARK LNITNAERLK
DYNELAVGDF VVHKNHGIGK YLGLQTLEVG GMHRDYLTIQ YQNGDTISVP VDHLDLLSKY
TAGEGKSPKI NKLNDGRWRK TMSSVSKQVE DISDDLIKLY AERQAKKGFA FSPDDASQEE
FDSGFSYVET EDQIRSINEI KHDMELERPM DRLLVGDVGF GKTEVAMRAA FKAINDGKQV
AVLVPTTVLA EQHFNNFTER FINFGVNVEV LSRFQTKTQQ TEILAKLKKG RVDLIIGTHR
LLSKDVEFFD LGLMIIDEEQ RFGVKHKERL KELKTQVDVL TLTATPIPRT LHMSMLGIRD
LSVIETPPTN RYPVQTYVME TNYGVVRDAI LREISRGGQV YYVYNRVDTI EQKVSQLEEL
IPEARIGFIH GQMTEVQLEN TLLAFIAGDY DVLVATTIIE TGVDIPNSNT LFIENADMMG
LSQLYQLRGR VGRSNRVAYA YFMYRPEKIL SEVSEKRLEA IKGFTELGSG FKIAMRDLSI
RGAGNLLGSE QSGFIDSVGF DLYSQLLEEA IHTKLGTSKQ KRTSNVEISL ELDAFIPAYY
ISDERQKIEI YKRIRQIDSR EIYEELQEEL VDRFGEYPDE VAYLLEIGLL KHFADNALVE
KIEKSRFEIT VTLEKVANTT YDPQDYFKAL AKTSMKASVG EKYGKMTFRL KIENRNSVVL
LSEIMNFVEE LSTIRDSYDK VR
//
