ID T1EF56_HELRO Unreviewed; 1047 AA.
AC T1EF56;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=Alpha-mannosidase {ECO:0000256|RuleBase:RU361199};
DE EC=3.2.1.- {ECO:0000256|RuleBase:RU361199};
GN Name=20195208 {ECO:0000313|EnsemblMetazoa:HelroP110840};
GN ORFNames=HELRODRAFT_110840 {ECO:0000313|EMBL:ESO07427.1};
OS Helobdella robusta (Californian leech).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC Hirudinea; Rhynchobdellida; Glossiphoniidae; Helobdella.
OX NCBI_TaxID=6412 {ECO:0000313|EnsemblMetazoa:HelroP110840, ECO:0000313|Proteomes:UP000015101};
RN [1] {ECO:0000313|Proteomes:UP000015101}
RP NUCLEOTIDE SEQUENCE.
RA Hellsten U., Grimwood J., Chapman J.A., Shapiro H., Aerts A., Otillar R.P.,
RA Terry A.Y., Boore J.L., Simakov O., Marletaz F., Cho S.-J.,
RA Edsinger-Gonzales E., Havlak P., Kuo D.-H., Larsson T., Lv J., Arendt D.,
RA Savage R., Osoegawa K., de Jong P., Lindberg D.R., Seaver E.C.,
RA Weisblat D.A., Putnam N.H., Grigoriev I.V., Rokhsar D.S.;
RL Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ESO07427.1, ECO:0000313|Proteomes:UP000015101}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23254933; DOI=10.1038/nature11696;
RA Simakov O., Marletaz F., Cho S.J., Edsinger-Gonzales E., Havlak P.,
RA Hellsten U., Kuo D.H., Larsson T., Lv J., Arendt D., Savage R.,
RA Osoegawa K., de Jong P., Grimwood J., Chapman J.A., Shapiro H., Aerts A.,
RA Otillar R.P., Terry A.Y., Boore J.L., Grigoriev I.V., Lindberg D.R.,
RA Seaver E.C., Weisblat D.A., Putnam N.H., Rokhsar D.S.;
RT "Insights into bilaterian evolution from three spiralian genomes.";
RL Nature 493:526-531(2013).
RN [3] {ECO:0000313|EnsemblMetazoa:HelroP110840}
RP IDENTIFICATION.
RG EnsemblMetazoa;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU361199};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|RuleBase:RU361199};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 38 family.
CC {ECO:0000256|ARBA:ARBA00009792, ECO:0000256|RuleBase:RU361199}.
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DR EMBL; AMQM01003675; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KB096222; ESO07427.1; -; Genomic_DNA.
DR RefSeq; XP_009014805.1; XM_009016557.1.
DR AlphaFoldDB; T1EF56; -.
DR STRING; 6412.T1EF56; -.
DR EnsemblMetazoa; HelroT110840; HelroP110840; HelroG110840.
DR GeneID; 20195208; -.
DR KEGG; hro:HELRODRAFT_110840; -.
DR CTD; 20195208; -.
DR eggNOG; KOG1958; Eukaryota.
DR HOGENOM; CLU_004690_1_0_1; -.
DR InParanoid; T1EF56; -.
DR OMA; VIMLERW; -.
DR OrthoDB; 5474711at2759; -.
DR Proteomes; UP000015101; Unassembled WGS sequence.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0004559; F:alpha-mannosidase activity; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006013; P:mannose metabolic process; IEA:InterPro.
DR GO; GO:0006491; P:N-glycan processing; IBA:GO_Central.
DR CDD; cd10809; GH38N_AMII_GMII_SfManIII_like; 1.
DR Gene3D; 3.20.110.10; Glycoside hydrolase 38, N terminal domain; 1.
DR Gene3D; 1.20.1270.50; Glycoside hydrolase family 38, central domain; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR011682; Glyco_hydro_38_C.
DR InterPro; IPR015341; Glyco_hydro_38_cen.
DR InterPro; IPR037094; Glyco_hydro_38_cen_sf.
DR InterPro; IPR000602; Glyco_hydro_38_N.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR013780; Glyco_hydro_b.
DR PANTHER; PTHR11607; ALPHA-MANNOSIDASE; 1.
DR PANTHER; PTHR11607:SF3; LYSOSOMAL ALPHA-MANNOSIDASE; 1.
DR Pfam; PF09261; Alpha-mann_mid; 1.
DR Pfam; PF07748; Glyco_hydro_38C; 1.
DR Pfam; PF01074; Glyco_hydro_38N; 1.
DR SMART; SM00872; Alpha-mann_mid; 1.
DR SUPFAM; SSF88688; Families 57/38 glycoside transferase middle domain; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361199};
KW Hydrolase {ECO:0000256|RuleBase:RU361199};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU361199};
KW Reference proteome {ECO:0000313|Proteomes:UP000015101};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU361199}.
FT DOMAIN 522..608
FT /note="Glycoside hydrolase family 38 central"
FT /evidence="ECO:0000259|SMART:SM00872"
FT COILED 56..83
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1047 AA; 120803 MW; A2C4ACC2A1F88815 CRC64;
MKFKIKRTLT LLIAIICVVL FLSMSFMMNN IDDGNIRDDR QPDDDKLVLL HFKDNILKLK
DQLDANEKKI DLLKDMLAQV SNQKLSKDDK LIPNLVAKRA TKNPIKFIKN ISLSSSLSVL
TCPQSTRYIA NSNADIQMSA VLDRVAFDNP DGGVWKQGYP ITYNENQWSE DNKLLVFVVP
HSHTDPGWIK TFDQYYQDQT SHILNLMVEK LVEYPKMRFV YAEMSFFTTW WQYASQNMRD
TVKKLLDEKR LEILTGGWVM SDEANAHYFA MLDQLIEGNQ WLSSELDYQP VTGWAIDPFG
YSPTLAYLLK SMSFENMLIQ RTHYSIKKEF SIKKDLEFMW RQQWDSKGST DILCHMMPFY
SYDIPHTCGP DPKICCQFDF RRMSGSRYSC PWRINPAPID DVNVHERAST LLDQYRKKSL
LYNSKVDGQH NNVVLIPLGD DFRYDTAEEW DLQYKNYEKL FDYMNQKKDW NVKAQFATLT
DYFTALRTRS AMNDRNFNSQ PQNVPFPSLS GDFFVYADRD DHYWSGYFTS RPFYKMLDRE
VEHHQRLAEI LFSLALSEAK HVDSSVLEKS DLSQLLIKSR RNLGLFQHHD GITGTAKDHV
VVDYGRRLLD SLNSLKKVII RSSEFLLSSM KSAFKFHDSL IFDLDETRQE HHDIPRPNVI
DLSMGKRTVY FFNSLGQSRK SIVTLHVTTA YVAVFDQAGR RVRSQISPKI LNTDGENLKI
SNHEFLLSFM VETLALGFSS FYLEEETADK KMVEFASVHV FNNKKISYDD DASSPNPFEI
THNDAAGPFI SNEQLQLRIS AESGLAKSIK WMDGSTNDER MELSFVKYGT THAKEKSGLY
LFIPGTEAEY LYTTYPTLTI TRGWLYSEAM SIFPLVTHHI RCSSIQSGLP GAGFDIMNIV
DVRGEGNTEI AMRMQTDVKN NDGTFYTDLN GFQVIKRKYL PKLPIQANFY PMPSMAYLQD
DSQRFSLLTA QSLGVASLKT GRLEVMLDRR LDQDDNRGAE QPLHDNKKTP NKFKIVIEKP
QNPPPTFNSD SQMGFFTPKS HFASLDL
//
