ID T1GHN0_MEGSC Unreviewed; 728 AA.
AC T1GHN0;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
OS Megaselia scalaris (Humpbacked fly) (Phora scalaris).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Platypezoidea;
OC Phoridae; Megaseliini; Megaselia.
OX NCBI_TaxID=36166 {ECO:0000313|EnsemblMetazoa:MESCA002938-PA, ECO:0000313|Proteomes:UP000015102};
RN [1] {ECO:0000313|Proteomes:UP000015102}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Durham, NC isolate 2 -- Noor lab
RC {ECO:0000313|Proteomes:UP000015102};
RA Hughes D.;
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:MESCA002938-PA}
RP IDENTIFICATION.
RG EnsemblMetazoa;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594}.
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DR EMBL; CAQQ02112321; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; T1GHN0; -.
DR STRING; 36166.T1GHN0; -.
DR EnsemblMetazoa; MESCA002938-RA; MESCA002938-PA; MESCA002938.
DR HOGENOM; CLU_001493_7_2_1; -.
DR OMA; DCWWNMS; -.
DR Proteomes; UP000015102; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000015102}.
FT DOMAIN 1..515
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 553..634
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 728 AA; 82577 MW; BFFBD8223D35A6A3 CRC64;
MNGKKINYIP GWDCHGLPIE LKALAEGTKA SPLEIRTKCA AFAKEALERQ KSGFKEWGVA
ADWELETSTY QTNRPGYVSN QLQLFYEFYQ KGLVFRDLKP VFWSPSSKSA LAEAELEYDN
NHESPSVFLQ LKLENVPDAF AVIWTTTPWT LPSNQAVCYN PSLEYALVYL DGNTSKKYLI
ATSLITSFSE TTNMTIDISE TISGKDLQGF RYQHPVDSKI LPFIAASHVE DSKGTGLVHT
APAHGPDDFL ISLEHKIPVL SLVDEEGKYT SKAPDFLVGK QVLEEGNKLV MEKASEQWFM
NTESLKTRAT EEIEKIKIYP RVNEEANRKM LMTQVQKRPY WCISRQRSWG VPIPVFYKKD
TKEIVLNREI VDCIANLVSK EGSIDFWWSK NPSEIISPDI LGRLEISPEN LVKGNDIFDI
WFDSGTSWSS VLGENKIADL YLEGYDQFTG WFQSSLLTSV AHRNCAPYKS LFVHGFTVDD
KGLKMSKSLG NVISPSEIIK KFGTDSLRWW VAAHGTQHMS ITVSNKLLQQ SAESVSKIRA
TLRYLNGVLG EGDRLYCGMD EDLAQIKSVL KECYLILNKA IWPVTPFLVE ESWSYFDSSA
PFYKNKSCVE NDWENKEAEQ IVTEALNLKR KINQMESTNS WLLHCSVNCT SEQLQYLSKL
QPKLSEFVNN SELCEILQVG SVKLEESMES SIELKISETP LCPRCRRFQC HQGGVCERCL
IVMKSKGF
//
