ID T1JW72_TETUR Unreviewed; 527 AA.
AC T1JW72;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Glucosylceramidase {ECO:0000256|ARBA:ARBA00012658, ECO:0000256|RuleBase:RU361188};
DE EC=3.2.1.45 {ECO:0000256|ARBA:ARBA00012658, ECO:0000256|RuleBase:RU361188};
OS Tetranychus urticae (Two-spotted spider mite).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Acariformes; Trombidiformes; Prostigmata; Eleutherengona; Raphignathae;
OC Tetranychoidea; Tetranychidae; Tetranychus.
OX NCBI_TaxID=32264 {ECO:0000313|EnsemblMetazoa:tetur02g07230.1, ECO:0000313|Proteomes:UP000015104};
RN [1] {ECO:0000313|Proteomes:UP000015104}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=London {ECO:0000313|Proteomes:UP000015104};
RA Rombauts S.;
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:tetur02g07230.1}
RP IDENTIFICATION.
RG EnsemblMetazoa;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|RuleBase:RU361188};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family.
CC {ECO:0000256|ARBA:ARBA00005382, ECO:0000256|RuleBase:RU361188}.
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DR EMBL; CAEY01000807; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; T1JW72; -.
DR STRING; 32264.T1JW72; -.
DR EnsemblMetazoa; tetur02g07230.1; tetur02g07230.1; tetur02g07230.
DR eggNOG; KOG2566; Eukaryota.
DR HOGENOM; CLU_014379_1_2_1; -.
DR OMA; AWAKYYV; -.
DR Proteomes; UP000015104; Unassembled WGS sequence.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006665; P:sphingolipid metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR033452; GH30_C.
DR InterPro; IPR001139; Glyco_hydro_30.
DR InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR11069; GLUCOSYLCERAMIDASE; 1.
DR PANTHER; PTHR11069:SF23; LYSOSOMAL ACID GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF02055; Glyco_hydro_30; 1.
DR Pfam; PF17189; Glyco_hydro_30C; 1.
DR PRINTS; PR00843; GLHYDRLASE30.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361188};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361188};
KW Lipid metabolism {ECO:0000256|RuleBase:RU361188};
KW Reference proteome {ECO:0000313|Proteomes:UP000015104};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Sphingolipid metabolism {ECO:0000256|RuleBase:RU361188}.
FT DOMAIN 102..456
FT /note="Glycosyl hydrolase family 30 TIM-barrel"
FT /evidence="ECO:0000259|Pfam:PF02055"
FT DOMAIN 459..522
FT /note="Glycosyl hydrolase family 30 beta sandwich"
FT /evidence="ECO:0000259|Pfam:PF17189"
SQ SEQUENCE 527 AA; 59638 MW; 723CE44AEF56FF6D CRC64;
MLLFVTLIHG NDDHCVKEYF DQNSFVCVCN ISVCPNIEPA TPVSKGYIDS WESSRDQYRF
HRTQLSINDL ARPLESNMLQ KLTLPYKKLR IQINIDDQRQ TIHGFGGAIT DATGLNLLNL
PVNLSDSIMS NYFSKNGLDY SVTRVPIGGT DFSTRIYTYA DDQPDDFELE HFALEPEDKL
LKIPLIKQAL KLKEGNLKLL GSAWSPPGWM KTNNNITGSG FLKGSPGGPY YKAWAKYYVK
FLEAYAKEGI DFWGITTQNE PTQAIVHQEF FYNCLGLTPQ QLRDFVKLDL GPVLRDAGYG
TDKLQVLLHD DIRDFLPSYT EAVLNDTEAA SYVTGIGYHW YKNGLEDFYE NLNLVHSNFP
NHFLFSTEAC EGFARFSTER AQLGNWTRAE TYAVDIIEDL NRFTTGWLDW NLALNTQGGP
SWAENWTDGL ILVDASKATY YRNPMFYSLG HFSKFIPPNS VYIGHEVQGD SQGVYVAAFI
DPSDQMVMVI LNTNDSPVDL IIEKFTSVSV PIAMDAHSIR TLITPRI
//
