ID T1U7T6_HELPX Unreviewed; 750 AA.
AC T1U7T6;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=Carbamoyltransferase {ECO:0000256|PIRNR:PIRNR006256};
DE EC=6.2.-.- {ECO:0000256|PIRNR:PIRNR006256};
GN Name=hypF {ECO:0000313|EMBL:AGT73299.1};
GN ORFNames=HPSA20_0054 {ECO:0000313|EMBL:AGT73299.1};
OS Helicobacter pylori SouthAfrica20.
OC Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=1352356 {ECO:0000313|EMBL:AGT73299.1, ECO:0000313|Proteomes:UP000015920};
RN [1] {ECO:0000313|EMBL:AGT73299.1, ECO:0000313|Proteomes:UP000015920}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SouthAfrica20 {ECO:0000313|EMBL:AGT73299.1};
RX PubMed=24072860;
RA Duncan S.S., Bertoli M.T., Kersulyte D., Valk P.L., Tamma S., Segal I.,
RA McClain M.S., Cover T.L., Berg D.E.;
RT "Genome Sequences of Three hpAfrica2 Strains of Helicobacter pylori.";
RL Genome Announc. 1:e00729-13(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl
CC phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE
CC protein] + diphosphate + H(+) + phosphate; Xref=Rhea:RHEA:55636,
CC Rhea:RHEA-COMP:14247, Rhea:RHEA-COMP:14392, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:76913,
CC ChEBI:CHEBI:139126, ChEBI:CHEBI:456215;
CC Evidence={ECO:0000256|ARBA:ARBA00001186};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acyl phosphate + H2O = a carboxylate + H(+) + phosphate;
CC Xref=Rhea:RHEA:14965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:43474, ChEBI:CHEBI:59918; EC=3.6.1.7;
CC Evidence={ECO:0000256|PROSITE-ProRule:PRU00520};
CC -!- PATHWAY: Protein modification; [NiFe] hydrogenase maturation.
CC {ECO:0000256|ARBA:ARBA00004711}.
CC -!- SIMILARITY: Belongs to the carbamoyltransferase HypF family.
CC {ECO:0000256|ARBA:ARBA00008097, ECO:0000256|PIRNR:PIRNR006256}.
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DR EMBL; CP006691; AGT73299.1; -; Genomic_DNA.
DR AlphaFoldDB; T1U7T6; -.
DR KEGG; hpys:HPSA20_0054; -.
DR PATRIC; fig|1352356.3.peg.49; -.
DR HOGENOM; CLU_009164_0_0_7; -.
DR UniPathway; UPA00335; -.
DR Proteomes; UP000015920; Chromosome.
DR GO; GO:0003998; F:acylphosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0016743; F:carboxyl- or carbamoyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR Gene3D; 3.30.110.120; -; 1.
DR Gene3D; 3.30.420.360; -; 1.
DR Gene3D; 3.30.420.40; -; 1.
DR Gene3D; 3.90.870.50; -; 1.
DR InterPro; IPR001792; Acylphosphatase-like_dom.
DR InterPro; IPR036046; Acylphosphatase-like_dom_sf.
DR InterPro; IPR017968; Acylphosphatase_CS.
DR InterPro; IPR004421; Carbamoyltransferase_HypF.
DR InterPro; IPR017945; DHBP_synth_RibB-like_a/b_dom.
DR InterPro; IPR041440; HypF_C.
DR InterPro; IPR006070; Sua5-like_dom.
DR InterPro; IPR011125; Znf_HypF.
DR NCBIfam; TIGR00143; hypF; 1.
DR PANTHER; PTHR42959; CARBAMOYLTRANSFERASE; 1.
DR PANTHER; PTHR42959:SF1; CARBAMOYLTRANSFERASE HYPF; 1.
DR Pfam; PF00708; Acylphosphatase; 1.
DR Pfam; PF17788; HypF_C; 1.
DR Pfam; PF01300; Sua5_yciO_yrdC; 1.
DR Pfam; PF07503; zf-HYPF; 2.
DR PIRSF; PIRSF006256; CMPcnvr_hdrg_mat; 1.
DR SUPFAM; SSF54975; Acylphosphatase/BLUF domain-like; 1.
DR SUPFAM; SSF55821; YrdC/RibB; 1.
DR PROSITE; PS00150; ACYLPHOSPHATASE_1; 1.
DR PROSITE; PS51160; ACYLPHOSPHATASE_3; 1.
DR PROSITE; PS51163; YRDC; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00520};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Transferase {ECO:0000313|EMBL:AGT73299.1};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 1..86
FT /note="Acylphosphatase-like"
FT /evidence="ECO:0000259|PROSITE:PS51160"
FT DOMAIN 192..376
FT /note="YrdC-like"
FT /evidence="ECO:0000259|PROSITE:PS51163"
FT ACT_SITE 16
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00520"
FT ACT_SITE 34
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00520"
SQ SEQUENCE 750 AA; 85140 MW; E1F9556B25D70E15 CRC64;
MNKITLFGVV QGVGMRPFIY TLAQKLELIG FVCNTQTALE IILPIQKTES FLNALKKELP
PLALVERIII SPYDKALHFN DFRILESKNH PLNLLSQIPK DLGVCDDCLH EIRDENSPYF
HYAFNSCAKC GARYSLLNAL PYDRENSALK PFKLCEFCAF VYKDTTNKRF HIQGISCKKC
GITLNYKQLN DDDALLECAK DIQKGKIIAL KGLGGFALVC DARNFQTIER LRLLKNRPLK
PFALMFKDLN TAKQYAFLNE LECESLRSAN APILLAHKKP NAQLAPNIAK NSPFYGIILP
YTPLHALLLD LLNFPIVFTS ANFNSLPLAS DEKELDRFHF IFDFKLTHNR AIIHRIDDSI
AQCVDNMIRP MRLARGFAPL YLTLPKRSHN APTKILALGA EQKGHFSLLD SETSVLLLSP
FCGDLSVLEN EKHFKETLNF FLNAYDFKPT LLACDEHKNY TTTKMACEFN APLLQVQHHH
AHFLASMLDA FLQDPNLNCP FIGIVWDGSG AYENKIYGAE CFVGDFNQIK ETARFEEFLL
LGGQKAIKDP KRLVLEISLK HQLNKLLKRV QKHFKEDEIE IFKQMHDKEI QSVATNSIGR
LFDIVAFSLG AVGTISFEAE SGQVLENLAL QSEESAFYPF KIKNSVVGLE EFYQALEKDL
DILEPKHIAK KFFNSLIEII TALIAPFKEH IVVCSGGVFC NQLLCEQLAK RFKTLQRKYF
FHKHFPPNDS SIPVGQALMA YFNPTIIKKG
//
